Significance of terminalαDgal(1-4)βDgal residues in urinary tract epithelium
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A monoclonal antibody to a terminal residue ofαDgal(1-4)βDgal, known as part of the receptor for p-fimbriatedEscherichia coli, was used to study the occurrence of this carbohydrate residue in urinary tract tissue from different animal species and man. Sections of bladder, ureter, and kidney tissue from man, monkey, guinea pig, rat, and mouse were investigated by an avidin-biotin-peroxidase staining method that proved useful for demonstrating carbohydrate structures in tissues. TerminalαDgal(1–4)βDgal residues were widely distributed in epithelial cells of the urinary tract in most of the species studied. Cells expressing this carbohydrate were also demonstrated in urinary sediment from healthy women. Experimental infection could not prove that this terminal structure alone was essential for establishment of pyelonephritis.
KeywordsEpithelial Cell Carbohydrate Monoclonal Antibody Urinary Tract Animal Species
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