Neutrophil-mediated injury to gastric mucosal surface cells
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Neutrophils (PMNs) have been implicated in the pathogenesis of gastritis. This study evaluates the magnitude and mode of PMN-mediated damage to gastric mucosal surface cells (GSC) in a system independent of vascular and neural factors. Rabbit GSC were freshly isolated and preloaded with51Cr. GSC were then incubated for 1 hr or 4 hr with freshly isolated human PMNs at varying effector-to-target cell ratios. Injury to GSC was assessed as percent specific51Cr released and by electron microscopy. We found minimal GSC injury using nonactivated PMNs. Incubation with PMNs activated with formylmethionyl-leucyl-phenalalanine (FMLP), however, resulted in significant GSC injury at the 20:1 PMN/GSC ratio, 33.2±1.8%51Cr release (P<0.001 compared to nonactivated PMNs). Electron microscopy revealed well-preserved gastric surface cells after exposure to nonstimulated PMNs. GSC exposed to activated PMNs (20:1 PMN/GSC ratio) were severely injured. Proteinase inhibitors and dimethylsulfoxide failed to diminish PMN-mediated GSC injury. Conversely, superoxide dismutase (SOD) inhibited GSC injury by more than 50% (P<0.001). In addition, glutathione peroxidase inhibited injury by 84% (P<0.001). These data suggest that neutrophil-mediated injury to gastric surface cellsin vitro involves superoxide anion and hypochlorous acid and not neutral trypsinlike proteinases or hydroxyl radicals.
Key wordsgastric mucosa neutrophils gastric mucosal injury gastritis free radicals
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- 3.Kozol R, Domanowski A, McCurdy G, Jaszewski R, Calzada R, Prasad M:Campylobacter pylori associated gastritis: A prospective comparison between histologic and endoscopic findings. Gastroenterology 96:A71, 1990Google Scholar
- 13.Nast CC, LeDuc LE: Chemotactic peptides, mechanisms and possible role in inflammatory bowel disease. Dig Dis Sci 33:505–575, 1988Google Scholar
- 14.Mutoh H, Hiraishi H, Ota S, Ivey KJ, Terano A, Sugimoto T: Role of oxygen radicals in ethanol-induced damage to cultured gastric mucosal cells. Am J Physiol 21:G603-G609, 1990Google Scholar
- 19.Yoshikawa T, Naito Y, Veda S, Oyamada H, Takemura T, Yoshida N, Sugino S, Kondo M: Role of oxygen derived free radicals in the pathogenesis of gastric mucosal lesions in the rats. J Clin Gastroenterol 12(suppl 1):565–571, 1990Google Scholar
- 21.Grisham MB, Granger DN: Neutrophil mediated mucosal injury, role of reactive oxygen metabolites. Dig Dis Sci 33:65–155, 1988Google Scholar
- 23.Granger DN, Hernandez LA, Grisham MB: Reactive oxygen metabolites: Mediators of cell injury in the digestive system. Viewpoints Dig Dis 19:13–16, 1986Google Scholar
- 26.Takemura T, Yoshikawa T, Yoshida N, Takano H, Tasaki N, Naito Y, Ueda S, Sugino S, Kondo M: Role of lipid peroxidation and oxygen radicals in compound 48/80 induced gastric mucosal injury in rats. Scand J Gastroenterol 24(suppl 162):51–54, 1989Google Scholar