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The effect of pH and urine dilution on the electrophoretic mobility of uric acid crystals

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Abstract

As a result of a continuing programme to understand better the uric acid stone treatment and prophylaxis, an investigation of the effect of pH and urine dilution on the surface charge of uric acid crystals was undertaken. A microelectrophoretic technique was employed to characterize the nature of the surface charge and the electrokinetics of uric acid crystals both in natural and synthetic urines under different conditions of pH and dilution. Both dilution and alkalization reduced the specific conductance of urine and increased the electrophoretic mobility (zeta potential) of uric acid crystals. The presence of cationic additives in diluted urine altered the zeta potential of uric acid crystals. Such findings suggest that proper control of the pH level and urine dilution as well as the surface charge at the solid-liquid interface represent an important factor in the uric acid stone prophylaxis.

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References

  1. Eason, A. A., Sharlip, I. D. Spaulding, J. T.: Dissolution of bilateral uric acid calculi causing anuria.JAMA, 240, 670 (1978).

    Google Scholar 

  2. Hardy, B., Klein, L. A.: In situ dissolution of ureteral calculus.Urology, 8, 444 (1976).

    Google Scholar 

  3. Neto, M., Pilloff, B., Simon, J. A.: Dissolution of renal uric acid calculus with allopurinol and alkalization of urine, a case report.J. Urol., 115, 740, (1976).

    Google Scholar 

  4. Petritsch, P. H.: Uric acid calculi: results of conservative treatment.Urology, 10, 536 (1977).

    Google Scholar 

  5. Ismail, S. I., Tawashi, R.: Effect of the organic matrix on the dissolution of uric acid stones.Eur. Urol., 6, 237 (1980).

    Google Scholar 

  6. Sennet, P., Olivier, J. P.: Colloidal dispersion, electrokinetic effects and the concept of zeta potential. In: Gushee, D. E. (ed.). Chemistry and Physics of Interfaces. American Chemical Society Publication. Washington, D. C. 1965, pp. 74–92.

    Google Scholar 

  7. Gardner, G. L., Doremus, R. H.: Crystal growth inhibitors in human urine; effect of calcium oxalate kinetics.Invest Urol., 15, 478 (1978).

    Google Scholar 

  8. Nash, R. A., Haeger, B. E.: Zeta potential in the development of pharmaceutical suspensions.J. Pharm. Sci., 55, 829 (1966).

    Google Scholar 

  9. Riddick, T. M.: Control of colloidal stability through zeta potential. Zeta-Meter Inc., New York 1968, pp. 320–331.

    Google Scholar 

  10. Zeta-Meter Manual ZM-77, 4th edition. Zeta-Meter Inc., New York 1977.

  11. Gottschalk, C. W., Mylle, M.: Micropuncture study of the mammalian urinary concentrating mechanism: Evidence for the countercurrent theory.Am. J. Physiol., 196, 927 (1959).

    Google Scholar 

  12. Leaf, A., Cotran, R. S.: Renal Pathophysiology. Oxford University Press, New York 1976, pp. 44–47.

    Google Scholar 

  13. Free, A. H., Free, H. M.: Urinalysis in Clinical Laboratory Practice. CRC Press Inc., Cleveland 1975, pp. 13–19.

    Google Scholar 

  14. Boyce, W. H., McKinney, W. M., Long, T. T., Drach, G. W.: Oral administration of methylene blue to patients with renal calculi.J. Urol., 97, 783 (1967).

    Google Scholar 

  15. Van't Riet, B., McKinney, W. M., Brandt, E. A., Currey, A. E., Taylor, D. M.: Dye effects on inhibition and dissolution of urinary calculi.Invest. Urol., 1, 446 (1964).

    Google Scholar 

  16. Rollins, R., Finlayson, B.: Mechanism of prevention of calcium oxalate encrustation by methylene blue and demonstration of the concentration dependence of its action.J. Urol., 110, 459 (1973).

    Google Scholar 

  17. Ahmad, K., Tawashi, R.: Methylene blue as an inhibitor of stone formation.Urol. Res., 6, 77 (1978).

    Google Scholar 

  18. Wein, A. J., Benson, G. S., Raezer, D. M., Mulholland, S. G.: Oral methylene blue and the dissolution of renal calculi.J. Urol., 116, 140 (1976).

    Google Scholar 

  19. Coe, F. L., Kavalach, A. G.: Hypercalciuria and hyperuricosuria in patients with calcium nephrolithiasis,N. Engl. J. Med., 291, 1344 (1974).

    Google Scholar 

  20. Meyer, J. L., Bergert, J. H., Smith, L. H.: The epitaxially induced crystal growth of calcium oxalate by crystalline uric acid.Invest. Urol., 14, 115 (1976).

    Google Scholar 

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Authors and Affiliations

  1. Faculté de Pharmacie, Université de Montréal, Canada

    S. I. Ismail, R. Tawashi & Z. Ismail

  2. School of Pharmaceutical Sciences, University Sains Malaysia, Minden, Penang, Malaysia

    S. I. Ismail, R. Tawashi & Z. Ismail

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  1. S. I. Ismail
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  2. R. Tawashi
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  3. Z. Ismail
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Ismail, S.I., Tawashi, R. & Ismail, Z. The effect of pH and urine dilution on the electrophoretic mobility of uric acid crystals. International Urology and Nephrology 17, 3–10 (1985). https://doi.org/10.1007/BF02089396

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  • DOI: https://doi.org/10.1007/BF02089396

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