Abstract
An analysis of the ultrastructural features of 35 canine kidneys after hypothermic preservation in Rheomacrodex, Collins3 and Sacks II solutions, and subsequent autologous reperfusion for 2 to 3 hours is presented. Signs of irreversibility (intramitochondrial crista fragmentation, cell membrane rupture, detachment of the brush borders in the area of the proximal tubular system) were confined to the rheomacrodex-preserved kidneys. Other abnormal features (cytoplasmic vacuolation, mitochondrial swelling in the proximal tubules, vacuolation of the visceral epithelial cells, heteromorphism in the glomerular system) found in the acute stage of revascularization may be regarded as reversible and account for the transitory functional disorders of renal transplants.
Similar content being viewed by others
References
Abouna, G. J. M.: Viability assays in organ preservation. Organ preservation for transplantation. Little, Brown and Co. Boston, 1974, p. 108.
Belzer, F. O., Downes, G. L.: Kidney. Organ preservation for transplantation. Little, Brown and Co. Boston, 1974, p. 298.
Ben-Ishay, Z., Wiener, J., Sweeting, J., Bradley, S. E., Spiro, D.: Fine structural alterations in the canine kidney during hemorrhagic hypotension.Lab. Invest., 17, 190 (1967).
Cook, M. L., Osvaldo, L., Jackson, D., Latta, H.: Changes in renal glomeruli during autolysis.Lab. Invest., 14, 623 (1965).
Craig, Sh. S., Seibel, H. R., Abouna, G. M.: Canine renal glomerular ultrastructural changes after preservation and autotransplantation.Invest. Urol., 14, 421 (1977).
Davies, D. J., Brewer, D. B., Hardwicke, J.: Urinary proteins and glomerular morphometry in protein overload proteinuria.Lab. Invest., 38, 232 (1978).
Ericsson, J. L. E.: Electron microscopy of renal tubule degeneration.Meth. Archiev. Exp. Path., Bajusz, E., Jasmin, G. (eds). Karger, Basel-New York 1969, Vol. 4, p. 171.
Fisher, E. R., Hellstrom, R.: Mechanism of proteinuria: Functional and ultrastructural correlation of effects of infusion of homologous and heterologous protein (bovine serum albumin) in the rat.Lab. Invest., 11, 617 (1962).
Fisher, E. R., Copeland, Ch., Fisher, B.: Correlation of ultrastructure and function following hypothermic preservation of canine kidneys.Lab. Invest. 17, 99 (1967).
Jeske, A. H., Abouna, G. M.: Ultrastructure of canine renal autografts following 24-hour hypothermic preservation.Europ. Surg. Res., 5, 424 (1973).
Jeske, A. H., Fonteles, M. C., Karow, A. M.: Functional preservation of the mammalian kidney. II. Ultrastructure with low flow perfusion at normothermia.J. Surg. Res., 15, 4 (1973).
Kastagir, B. K., Kabb, K., Leonards, J. R.: Ultrastructure in the canine kidneys preserved 24 hours.Trans. Amer. Soc. Artif. Int. Organs, 15, 214 (1969).
Latta, H., Osvaldo, L., Jackson, J. D., Cook, M. L.: Changes in renal cortical tubules during autolysis.Lab. Invest., 14, 635 (1965).
Mackay, B., Moloney, P. J., Rix, D. B.: The use of electron microscopy in renal preservation and perfusion. In: Norma, J. C. (ed.): Organ Perfusion and Preservation. Appleton-Century-Crofts, New York 1968, p. 697.
Mendel, D.: Tubular reabsorption of protein in rats with experimental proteinuria.J. Physiol., 156, 544 (1961).
Oliver, J.: Correlation of structure and function and mechanism of recovery in acute tubular necrosis.Amer. J. Med., 15, 535 (1953).
Osborne, D. G., Magnusson, M. O., Kiser, W. S. Hawk, W. A.: Timed sequential ultrastructural changes of canine kidneys unflushed and after flushing with plasma. Proc. 29th Ann. EMSA Meeting, 1970, p. 314.
Pausescu, E., Cotutiu, C., Tomescu, E., Patru, A.: Electron optic morphological and cytochemical evidence in support of a “cytoplasmic” solution for kidney preservation.Europ. Surg. Res., 5, 438 (1973).
Romhányi, Gy., Deák, Gy.: On the ultrastructural organization of biological membranes as shown by topo-optical staining reactions.Acta morph. Acad. Sci. hung. 17, 245 (1969).
Rőth, E., Tóth, I., Török, B.: Histological features of stored kidneys after simple hypothermic perfusion with preserving solutions of different types.Int. Urol. Nephrol., 6, 243 (1974).
Rőth, E., Deák, Gy., Szmolenszky, T.: Description and interpretation of structural changes in canine kidneys after preservation with a hyperosmolar solution and reperfusion.Int. Urol. Nephrol., 9, 185 (1977).
Rőth, E., Szmolenszky, T., Török, B.: Description and interpretation of functional changes in canine kidneys after preservation with hypothermic perfusates and subsequent reperfusion.Int. Urol. Nephrol., 10, 335 (1978).
Rőth, E., Szmolenszky, T., Török, B.: Description and interpretation of morphological changes in canine kidneys after preservation with hypothermic solutions and subsequent reperfusion.Int. Urol. Nephrol., 11, 335 (1979).
Rouiller, Ch., Muller, A. F.: The Kidney. I. Morphology, Biochemistry, Physiology. Academic Press, New York-London 1969, p. 262.
Sandborn, E. B.: Cells and Tissues by Light and Electron Microscopy. II. Academic Press, New York-London, 1970, p. 134.
Skaaring, P., Bierring, F., Hejnal, J., Svendsen, V., Jensen, K., Kemp, E. Ultrastructure of the glomerular filtration membrane of autotransplanted canine kidneys stored for 24 hours.Cryobiology, 12, 224 (1975).
Török, B., Rőth, E., Szmolenszky, T.: Observations on the donor kidney and their interpretation. II. Structural and functional changes in the acute stage of reperfusion.Acta chir. Acad. Sci. hung. (in press).
Vernier, R. L.: Ultrastructure of the glomerulus and changes in fine structure associated with increased permeability of the glomerulus to protein. Ciba Foundation Symposium on Renal Biopsy. Little Brown and Co., Boston 1961, p. 4.
Warnick, C. T., Lazarus, H. M.: Adenine nucleotides during organ storage.Transplant. Proc., IX, 1575 (1977).
Waugh, D., Schlieter, W., James, A. W.: Infraglomerular epithelial reflux.Arch. Pathol., 77, 101 (1964).
Author information
Authors and Affiliations
Additional information
Supported by the Scientific Research council, Ministry of Health, Hungary (3-09-0600-01/T).
Rights and permissions
About this article
Cite this article
Rőth, E., Szmolenszky, T. & Török, E. Ultrastructural features of canine kidneys after preservation with hyperthermic solutions and subsequent reperfusion. International Urology and Nephrology 12, 137–151 (1980). https://doi.org/10.1007/BF02089353
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02089353