Digestive Diseases and Sciences

, Volume 41, Issue 9, pp 1864–1870 | Cite as

Total parenteral nutrition impairs bile flow and alters bile composition in newborn piglet

  • Donald R. Duerksen
  • John E. van Aerde
  • George Chan
  • Alan B. R. Thomson
  • Laurence J. Jewell
  • Michael T. Clandinin
Growth, Development, And Nutrition


Cholestatic liver disease complicates total parenteral nutrition (TPN) in premature neonates. We investigated TPN-induced liver disease in the newborn piglet, hypothesizing that: (1) TPN impairs bile flow by reducing the bile acid-dependent (BADF) and the bile acid-independent component of bile flow (BAIF); and (2) TPN changes bile composition. For three weeks, eight piglets received TPN and nine piglets were fed milk. Basal bile flow was measured and bile composition analyzed for bile acids, cholesterol (C), phospholipids (PL), and PL fatty acids. Bile flow was also measured after stimulation with 20, 50, and 100 µmol/kg taurocholic acid (TCA). Liver histology and bilirubin content were examined. Basal bile flow was reduced from 11.6±1.2 µl/g liver/10 min in orally fed animals to 1.6±0.4 µl/g liver/10 min in the TPN group. The stimulated bile flow in the TPN group did not respond to TCA and was lower than in the orally fed animals at each TCA dose. Both BADF and BAIF were significantly lower in the TPN group. Bile acid secretion was less than 50% of control values and total C and PL secretions were less than 5% of control. Liver and serum bilirubin were elevated in the TPN group. The newborn piglet is a valid model to study TPN-induced cholestasis, characterized by decreased bile acid secretion, impaired BADF and BAIF, and reduced bile flow stimulation after intravenous TCA.

Key words

total parenteral nutrition cholestasis bile acids bile flow 


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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Donald R. Duerksen
    • 1
    • 2
    • 3
  • John E. van Aerde
    • 1
    • 2
    • 3
  • George Chan
    • 1
    • 2
    • 3
  • Alan B. R. Thomson
    • 1
    • 2
    • 3
  • Laurence J. Jewell
    • 1
    • 2
    • 3
  • Michael T. Clandinin
    • 1
    • 2
    • 3
  1. 1.From the Department of Pediatrics, Nutrition and Metabolism Research Group, Perinatal Research CentreUniversity of AlbertaEdmontonCanada
  2. 2.Department of Medicine, Nutrition and Metabolism Research Group, Perinatal Research CentreUniversity of AlbertaEdmontonCanada
  3. 3.Department of Pathology, Nutrition and Metabolism Research Group, Perinatal Research CentreUniversity of AlbertaEdmontonCanada

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