Lead as an inductor of some morphological and functional changes in synaptosomes from rat brain
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1. The effect of lead (in vivo) on the uptake of GABA, dopamine, and histidine as a precursor of histamine in synaptosomes obtained from chronically lead-treated rats was studied.
2. Lead decreased the uptake of GABA, increased the uptake of dopamine, and did not change the uptake of histidine. These effects were independent of calcium concentration.
3. Lead administration to the rat changed the morphology of the synaptosomes, as manifested in the decreased number of synaptic vesicles and disturbed mitochondrial structure.
4. The results suggest the existence of several mechanisms of lead toxicity on uptake, related to individual neurotransmitters, which are not necessarily connected with a Pb2+/Ca2+ interaction.
Key wordslead brain synaptosomes neurotransmitter uptake
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- Becker, D. M., Viljoen, J. D., and Kramer, S. (1976). InPorphyrins in Human Diseases (M. Doss, Ed.), Karger, Basal, pp. 163–172.Google Scholar
- Booth, R. F., and Clark, J. B. (1978). A rapid method for the preparation of relatively pure, metabolically competent synaptosomes from rat brain.Biochem. J. 176:365–370.Google Scholar
- Deutsch, C., Drawn, C., Rafałowska, U., and Silver, I. A. (1981). Synaptosomes from rat brain: Morphology, compartmentation and transmembrane pH and electrical gradients.J. Neurochem. 36:2063–2072.Google Scholar
- Guilarte, T. R. (1993). Neurochemical aspects of hippocampal and cortical lead neurotoxicity. Abstracts of 4th Meeting of the International Neurotoxicology, Elsinase, Denmark, p. 12.Google Scholar
- Hypponen, S., Kohila, T., and Tahti, H. (1993). The effect of cadmium lead and aluminium on neural cell membrane adenosino-triphosphatase activity.Neurosci. Res. Commun. 12:77–84.Google Scholar
- Jabłońska, L., and Rafałowska, U. (1992a). Differences in action of lead on uptake and release of GABA, dopamine, histamine and histidine in rat brain synaptosomes. 1st International Congress of the Polish Neuroscience Society, Warsaw.Google Scholar
- Jabłońska, L., and Rafałowska, U. (1992b). Lead action on synaptosome neurotransmission in vivo and in vitro. 9th Meeting of the European Society for Neurochemistry, Dublin, Ireland.Google Scholar
- Kalton, L., and Yaasi, Y. (1982). Sites of action of lead on spontaneous transmitter release from motor nerve terminals.Israel J. Med. Sci. 18:165–170.Google Scholar
- Kater, B. K., Mattson, M. P., Cohan, C., and Connor (1988). Calcium regulation of the neuronal growth cone.TINS 11:315–321.Google Scholar
- Lowry, O. H., Rosebrough, A. L., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.Google Scholar
- Lynch, G., Larson, J., Kelso, S., Barsuionuezo, G., and Schattler, F. (1983). Intracellular injection of EGTA blocks induction of hippocampal long-term potentiation.Nature (Lond.) 302:432–434.Google Scholar
- Needleman, H. L. (1980).Low Level Lead Exposure. The Clinical Implications of Current Research, Raven Press, New York.Google Scholar
- Neseratham, S., Bawa, S., and Scutton, M. C. (1992). Lack of acute effect of Pb2+ on platelet aggregation. Thrombosis and haemostasis.Life Sci. 68:373.Google Scholar
- Pastuszko, A., Wilson, D., and Erecińska, M. (1981). Net uptake of γ-amino-butyric acid by a high-affinity system of rat brain synaptosomes.Proc. Natl. Acad. Sci. USA 78(2):1242–1244.Google Scholar
- Rafałowska, U., and Wałajtys-Rode, E. (1991). Peroxidation-induced changes of histamine metabolism and transport of its precursor histidine in rat brain synaptosomes.Free Radical Biochem. Med. 10:23–28.Google Scholar
- Rafałowska, U., Erecińska, M., and Wilson, D. (1980). Energy metabolism in rat brain synaptosomes from nembutal-anesthetized and nonanesthetized animals.J. Neurochem. 34:1380–1386.Google Scholar
- Rafałowska, U., Zitting, A., and Savolainen, H. (1986). Metabolic changes in rat brain synaptosomes after exposure to sulfide in vivo.Toxicol. Lett. 43:193–200.Google Scholar
- Rafałowska, U., Waśkiewicz, J., and Albrecht, J. (1987). Is neurotransmitter histamine predominantly inactivated by astrocytes?Neurosci. Lett. 80:106–110.Google Scholar
- Rafałowska, U., Lin, G. J., and Floyd, R. A. (1989). Peroxidation induced changes in synaptosomal transport of dopamine and γ-aminobutyric acid.Free Radical Biol. Med. 6:485–492.Google Scholar
- Schanne, F. A. X., Moskal, J. R., and Gupta, R. K. (1989). Effect of lead on intracellular free calcium ion concentration in a presynaptic neuronal model: 19F-NMR study of NG108-15 cells.Brain Res. 503:308–311.Google Scholar
- Silbergeld, E. K. (1982). InMechanisms of Action of Neurotoxic Substances (K. N. Prasad and A. Vernadakis, Eds.), Raven Press, New York, pp. 1–25.Google Scholar
- Silbergeld, E. K. (1983). Localization of metals: Issues of importance to neurotoxicology of lead.Neuro Toxicology 4(3):193–200.Google Scholar
- Silbergeld, E. K., and Lamon, J. R. (1980). Role of altered heme synthesis in lead neurotoxicity.J. Occup. Med. 22:680–684.Google Scholar
- Silbergeld, E. K., Fales, J. T., and Goldberg, A. M. (1974). Lead: evidence for a prejunctional effect on neuromuscular function.Nature 247:49–50.Google Scholar
- Silinsky, E. M. (1985). The biophysical pharmacology of calcium-dependent acetylcholine secretion.Pharmacol. Rev. 37:81–132.Google Scholar
- Strużyńska, L., and Rafałowska, U. (1994a). The effect of lead on dopamine GABA and histidine spontaneous and KCl-dependent releases from rat brain synaptosomes.Acta Neurobiol. Exp. 54:1–7.Google Scholar
- Strużyńska, L., and Rafałowska, U. (1994b). Alterations in energy metabolism of synaptosomes caused by chronic lead administration. European Society for Neurochemistry, 10th ESN Meeting, Jerusalem, Abstract.Google Scholar
- Troeger, M. B., Rafałowska, U., and Erecińska, M. (1984). Effect of oleate on neurotransmitter transport and the plasma membrane functions in rat brain synaptosomes.J. Neurochem. 6:1735–1742.Google Scholar
- Tschudy, D. P., and Lamon, I. M. (1980). InMetabolic Disease and Control, 8th ed. (P. K. Bandy and L. E. Rosenberg, Eds.), W. B. Saunders, Philadelphia, PA, pp. 939–1007.Google Scholar
- Wałajtys-Rode, E., Waśkiewicz, J., and Rafałowska, U. (1989). Effect of in vivo glucocorticoid administration on histamine metabolism in rat brain synaptosomes.Neurosci. Lett. 97:340–344.Google Scholar
- Zareba, G., and Chmielnicka, J. (1992). Disturbances in heme biosynthesis in rabbits after administration per os of low doses of tin or lead.Biol. Trace Element Res. 34(2):115–122.Google Scholar