Cellular and Molecular Neurobiology

, Volume 14, Issue 1, pp 1–7 | Cite as

Expression of mRNA for atrial natriuretic peptide receptor guanylate cyclase (ANPRA) in human retina

  • Komanduri Pardhasaradhi
  • R. Krishnan Kutty
  • Susan Gentleman
  • Gopal Krishna
Article

Summary

1. Guanylate cyclase plays an important role in the visual cycle. Here we report the mRNA expression for the atrial natriuretic peptide receptor type A form of guanylate cyclase (ANPRA) in human retina.

2. Polymerase chain reaction using two sets of primers on the cDNAs reverse-transcribed from human retinal poly(A)+ RNA amplified two products under two different reaction conditions. The primers used in the reaction were designed from the reported sequence of human placental ANPRA cDNA.

3. Sequencing of the amplified products showed 100% sequence homology to the human placental ANPRA gene. Northern blot analysis indicated the presence of a 4.4-kb ANPRA mRNA in human retina, similar to that present in human brain.

Key words

atrial natriuretic peptide receptor type A guanylate cyclase polymerase chain reaction human retina northern blot 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Komanduri Pardhasaradhi
    • 2
  • R. Krishnan Kutty
    • 2
  • Susan Gentleman
    • 1
  • Gopal Krishna
    • 2
  1. 1.Laboratory of Retinal Cell and Molecular BiologyNational Eye Institute, NIHBethesda
  2. 2.Section on Drug-Tissue Interaction, Laboratory of Chemical PharmacologyNHLBI, National Institute of HealthBethesda

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