Digestive Diseases and Sciences

, Volume 39, Issue 3, pp 526–533 | Cite as

Study of colonic IgG Fc binding site in cultured epithelial cells

  • Kensuke Kobayashi
  • William R. Brown
Original Articles

Abstract

Previously, we identified a unique binding site for the Fc region of IgG in goblet cells of the human small intestine and colon. Understanding of the regulation and biological role of the binding site would be enhanced if it could be studied in cultured cells. Thus, we now have searched colonic carcinoma cell lines for presence of the site and further characterized it in such cells. HT29 and HT29-18N2 (a mucin-secreting subclone) cells were capable of binding IgG. The binding was associated with mucus-producing cells only in the cultures, was enhanced by culture of the cells in galactose-containing medium (which favors mucin production) as compared to glucose-containing medium, and was much greater for the HT29-18N2 cells than for the HT29 cells. LS 174T cells did not bind IgG despite the presence of numerous mucin-containing cells, and Colo 205 and LoVo cell cultures had neither mucin-containing cells nor IgG binding; thus, IgG binding and mucin production are not inseparably linked. By use of monoclonal antibodies to three different molecular-size components of the binding site, we found that a >200-kDa component probably is necessary but not sufficient for IgG binding, whereas 78-kDa and 110- to 140-kDa components are not necessary.

Key words

goblet cells Fc receptors mucin mucus immunoglobulin 

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References

  1. 1.
    Neutra NR, Forstner JF: Gastrointestinal mucus: Synthesis, secretion, and function.In Physiology of the Gastrointestinal Tract, 2nd ed. LR Johnson (ed). New York, Raven Press, 1987, pp 975–1009Google Scholar
  2. 2.
    Kobayashi K, Blaser MJ, Brown WR: Identification of a unique IgG Fc binding site in human intestinal epithelium. J Immunol 143:2567–2574, 1989PubMedGoogle Scholar
  3. 3.
    Kobayashi K, Hamada Y, Blaser MJ, Brown WR: Characterization of the molecular configuration and ultrastructural locations of an IgG Fc binding site in human colonic epithelium. J Immunol 146:68–74, 1991PubMedGoogle Scholar
  4. 4.
    Pinto M, Appay MD, Simon-Assmann P, Chevalier G, Dracopoli N, Fogh J, Zweibaum A: Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by galactose in the medium. Biol Cell 44:193–196, 1982Google Scholar
  5. 5.
    Pinto M, Robine-Leon S, Appay MD, Kedinger M, Triadou N, Dussaulx E, Lacroix B, Simon-Assmann P, Haffen K, Fogh J, Zweibaum A: Enterocyte-like differentiation and polarization of the human colon carcinoma cell line CaCo-2 in culture. Biol Cell 47:323–330, 1983Google Scholar
  6. 6.
    Zweibaum A, Pinto M, Chevalier G, Dussaulx E, Triadou N, Lacroix B, Haffen K, Brun JL, Rousset M: Enterocytic differentiation of a subpopulation of the human colon tumor cell line HT-29 selected for growth in sugar-free medium and its inhibition by glucose. J Cell Physiol 122:21–29, 1985PubMedGoogle Scholar
  7. 7.
    Huet C, Sahuquillo-Merino C, Coudrier E, Louvard D: Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT29) provide new models for cell polarity and terminal differentiation. J Cell Biol 105:345–356, 1987PubMedGoogle Scholar
  8. 8.
    Phillips TE, Huet C, Bilbo PR, Podolsky DK, Louvard D, Neutra MR: Human intestinal goblet cells in monolayer culture: Characterization of a mucus-secreting subclone derived from the HT29 colon adenocarcinoma cell line. Gastroenterology 94:1390–1403, 1988PubMedGoogle Scholar
  9. 9.
    Hamada Y, Kobayashi K, Brown WR: Abnormalities in expression of the intestinal IgG Fc binding site (I-FcBS) in ulcerative colitis (UC) and the cottone-top tamarin (CTT). Gastroenterology 98:AGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Kensuke Kobayashi
    • 1
    • 2
  • William R. Brown
    • 1
    • 2
  1. 1.From the Department of MedicineVeterans Affairs Medical Center and University of Colorado School of MedicineDenver
  2. 2.The Department of Internal Medicine, Ichikawa General HospitalTokyo Dental CollegeChibaJapan

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