Skip to main content
Log in

Mucosal concentrations of interleukin-1β, interleukin-6, interleukin-8, and tumor necrosis factor-α in pelvic ileal pouches

  • Original Articles
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Concentrations of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were determined by solid-phase ELISA in tissue homogenates of mucosal biopsy specimens obtained from pelvic ileal pouches in 13 patients with pouchitis (reservoir ileitis) and 17 with pouches without pouchitis. Normal ileal mucosa was used as a control. IL-1β was detected in all tissue homogenates from patients with pouchitis compared with only 29% from pouches without pouchitis and none from controls. IL-6 and IL-8 were present in all pouchitis specimens, in 70% of the specimens from nonpouchitis and only 30% of specimens from controls. TNF-α was undetectable in all specimens examined. The concentrations of IL-1β, IL-6, and IL-8 were significantly greater (P<0.001) in biopsy specimens from pouchitis compared to those from pouches without pouchitis or normal ileal mucosa and in patients with pouchitis tissue levels of IL-1β significantly correlated with IL-6 (P<0.05) and IL-8 (P<0.01). Furthermore IL-1 and IL-8 levels were significantly higher in tissue specimens from nonpouchitis pouches than in those from normal ileal mucosa (P<0.02). These results suggest that an enhanced cellular immunity operatesin vivo at the mucosal level in pouchitis as in the case of ulcerative colitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Parks AG, Nicholls RJ: Proctocolectomy without ileostomy for ulcerative colitis. Br Med J 2:85–88, 1978

    Google Scholar 

  2. Taylor BA, Dozois R: The J ileal pouch anastomosis. World J Surg 11:727–733, 1987

    Google Scholar 

  3. Tytgat GNJ, Van Deventer SJH: Pouchitis. Int J Colorect Dis 3:226–228, 1988

    Google Scholar 

  4. Madden MV, Farthing MJG, Nicholls RJ: Inflammation in ileal reservoirs pouchitis. Gut 31:247–249, 1990

    Google Scholar 

  5. Moskowitz RJ, Sheperd NA, Nicholls RJ: An assessment of inflammation in the reservoir after restorative proctocolectomy with ileoanal reservoir. Int J Colorect Dis 1:167–174, 1986

    Google Scholar 

  6. Meuwissen SGM, Hoitsma H, Boot H, Seldenrijk CA: Pouchitis (pouch ileitis). Neth J Med 35:S54-S66, 1989

    Google Scholar 

  7. Tytgat GNJ: Workshop. Pouchitis. Int J Colorect Dis 4:205–229, 1989

    Google Scholar 

  8. De Silva HJ, Jones M, Prince C, Kettlewell M, Mortensen NJ, Jewell DP: Lymphocyte and macrophage subpopulations in pelvic ileal pouches. Gut 32:1160–1165, 1991

    Google Scholar 

  9. Van Spreeuvel JP, Lindeman J, Meijer CJLM: A quantitative study of immunoglobulin containing cells in the differential diagnosis of acute colitis. J Clin Pathol 38:774–777, 1985

    Google Scholar 

  10. Allison MC, Cornwall S, Poulter LW, Dhillon AP, Pounder RE: Macrophage heterogeneity in normal colonic mucosa and inflammatory bowel disease. Gut 29:1531–1538, 1988

    Google Scholar 

  11. Mahida YR, Patel S, Gionchetti P, Jewell DP: Macrophage subpopulations in the lamina propria of normal and inflamed colon and terminal ileum. Gut 30:826–834, 1989

    Google Scholar 

  12. Rees RC: Cytokines as biological response modifiers. J Clin Pathol 45:93–98, 1992

    Google Scholar 

  13. Peterson GL: A simplification of the protein assay method of Lowry et al which is more generally applicable. Anal Biochem 83:346–356, 1987

    Google Scholar 

  14. Gaffney EW, Chu CW, Lingenfelter SE, Lisi PJ, Koch GA: Enzyme linked immunoassay with monoclonal antibody for human interleukin-1. Biotechniques 5:652–656, 1987

    Google Scholar 

  15. Akira S, Hirano T, Taga T, Kishimoto T: Biology of multifunctional cytokines: IL-6 and related molecules (IL-1 and TNF). FASEB J 4:2860–2867, 1990

    Google Scholar 

  16. Philip R, Epstein LB: Tumour necrosis factor as immunomodulatory and mediator of monocyte citotoxicity induced by itself, γ interferon and interleukin 1. Nature 323:86–89, 1986

    Google Scholar 

  17. Dinarello CA, Canon JG, Wolff SM, Bernheim HA, Beutler B, Cerami A, Figari IS, Palladino MA, Jr, Connor JV: Tumour necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med 163:1433–1450, 1986

    Google Scholar 

  18. Sironi M, Breviario F, Proserpio P, Biondi A, Vecchi A, Van Damme J, Dejana E, Mantovani A: IL-1 stimulates IL-6 production in endothelial cells. J Immunol 142:549–553, 1989

    Google Scholar 

  19. Baggiolini M, Walz A, Kunkel SL: Neutrophil activating peptide 1/interleukin 8, a novel cytokhine that activates neutrophils. J Clin Invest 84:1045–1049, 1989

    Google Scholar 

  20. Mahida YR, Wu K, Jewell DP: Enhanced production of interleukin 1β by mononuclear cells isolated from mucosa with active ulcerative colitis or Crohn's disease. Gut 30:835–838, 1989

    Google Scholar 

  21. Ligumsky M, Simon PL, Karmeli F, Rachmilewitz D: Role of interleukin 1 in inflammatory bowel disease: Enhanced production during active disease. Gut 31:686–689, 1990

    Google Scholar 

  22. Brinskov J, Tvede N, Andersen CB, Vilien M: Increased concentration of interleukin 1β, interleukin 2 and soluble interleukin 2 receptor in endoscopical mucosal biopsy specimen with active inflammatory bowel disease. Gut 33:55–58, 1992

    Google Scholar 

  23. Pullman WE, Elsbury S, Kobayashi M, Hapel AJ, Doe WF: Enhanced mucosal cytokine production in inflammatory bowel disease. Gastroenterology 102:529–537, 1992

    Google Scholar 

  24. Nakamura M, Saito H, Kasanuki J, Tamura Y, Yoshida S: Cytokine production in patients with inflammatory bowel disease. Gut 33:933–937, 1992

    Google Scholar 

  25. Dinarello CA: Interleukin 1. Dig Dis Sci 3(suppl):25S-35S, 1988

    Google Scholar 

  26. Rachmilewitz D, Simon PL, Schwartz LW, Griswald DE, Fondacaro JD, Wasserman MA: Inflammatory mediators of experimental colitis in rats. Gastroenterology 97:326–337, 1989

    Google Scholar 

  27. Cominelli F, Nast CC, Clark BD, Schindler R, Llerena R, Eysselein VE, Thompson RC, Dinarello CA: Interleukin 1 (IL-1) gene expression, synthesis, and effect of specific IL-1 receptor blockade in rabbit immune complex colitis. J Clin Invest 86:972–980, 1990

    Google Scholar 

  28. Mitsuyama K, Sasaky E, Toyonaga A, Ikeda H, Tsuruta O, Irie A, Arima N, Oriishi T, Harada K, Fujisaki K, Sata M, Tanikawa K: Colonic mucosal interleukin 6 in inflammatory bowel disease. Digestion 50:104–111, 1991

    Google Scholar 

  29. Mahida YR, Ceska M, Effenberg F, Kurlak L, Lindley I, Hawkey CJ: Enhanced synthesis of neutrophil-activating peptide 1/interleukin 8 in active ulcerative colitis. Clin Sci 82:273–275, 1992

    Google Scholar 

  30. Bazzoni F, Cassatella MA, Rossi F, Ceska M, Dewald B, Baggiolini M: Phagocytosing neutrophils produce and release high amounts of neutrophil activating peptide 1/interleukin 8. J Exp Med 173:771–774, 1991

    Google Scholar 

  31. Elner VM, Strieter RM, Elner SG, Baggiolini M, Lindley I, Kunkel SL: Neutrophil chemotactic factor (IL-8) gene expression by cytokine treated retinal pigment epithelial cells. Am J Pathol 136:745–750, 1990

    Google Scholar 

  32. Mahida YR, Wu K, Lamming CED, Jewell DP, Hawkey CJ: Human colonic tumour necrosis factor α (TNFα) production. Gastroenterology 96:A313, 1989

    Google Scholar 

  33. Mazlam MZ, Hodgson HJF: Peripheral blood monocyte cytokine production and acute phase response in inflammatory bowel disease. Gut 33:773–778, 1992

    Google Scholar 

  34. McDonald TT, Hutchings P, Choy MY, Murch S, Cook A: Tumour necrosis factor α and interferon γ production measured at the single cell level in normal and inflamed human intestine. Clin Exp Immunol 81:301–305, 1990

    Google Scholar 

  35. Mahida YR, Scott E, Kurlak L, Gallagher A, Hawkey CJ: Interleukin 1β, tumour necrosis factor α and interleukin 6 synthesis by circulating mononuclear cell isolated from patients with active ulcerative colitis. Eur J Gastroenterol 4:501–507, 1992

    Google Scholar 

  36. Isaacs KL, Sartor BR, Haskill S: Cytokine messenger RNA profiles in inflammatory bowel disease detected by polymerase chain reaction amplification. Gastroenterology 103:1587–1595, 1992

    Google Scholar 

  37. Sheperd NA, Jass JR, Duval I, Moskowitz RL, Nicholls RJ, Morson BC: Restorative proctocolectomy with ileal reservoir: Pathological and histochemical study of mucosal biopsy specimens. J Clin Pathol 40:601–607, 1987

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gionchetti, P., Campieri, M., Belluzzi, A. et al. Mucosal concentrations of interleukin-1β, interleukin-6, interleukin-8, and tumor necrosis factor-α in pelvic ileal pouches. Digest Dis Sci 39, 1525–1531 (1994). https://doi.org/10.1007/BF02088059

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02088059

Key words

Navigation