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Regulation of hepatocyte albumin and α1-acid glycoprotein secretion by monokines, dexamethasone, and nitric oxide synthase pathway: Significance of activated liver nonparenchymal cells

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Abstract

To clarify the mechanism involved in regulating the secretion of albumin and α1-acid glycoprotein by rat hepatocytes, we studied hepatocyte culture and cocultures of hepatocyte and liver nonparenchymal cells. The secretion of α1-acid glycoprotein by hepatocytes was stimulated and that of albumin was inhibited by combinations of dexamethasone and monokines, especially by dexamethasone and interleukin-6. The secretion of these proteins was equally inhibited during stimulation by lipopolysaccharide in cocultures. The inhibitory effect of sinusoidal endothelial cells was smaller than that of Kupffer cells. This inhibition was partially aboslished by blocking the nitric oxide synthase pathway in cocultured cells and was completely abolished by dexamethasone. In conclusion, the secretion of albumin and α1-acid glycoprotein by hepatocytes was regulated by monokines, dexamethasone, and the inducible nitric oxide synthase pathway in hepatocytes and liver nonparenchymal cellsin vitro.

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Authors and Affiliations

  1. Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyouku, 602, Kyoto, Japan

    Yoshito Itoh MD,  Takeshi Okanoue MD, Fumio Enjo MD, Shinichi Sakamoto MD, Shirou Takami MD, Kouichirou Yasui MD, Keizo Kagawa MD & Kei Kashima MD

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  1. Yoshito Itoh MD
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  2. Takeshi Okanoue MD
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  3. Fumio Enjo MD
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  4. Shinichi Sakamoto MD
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  5. Shirou Takami MD
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  6. Kouichirou Yasui MD
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  7. Keizo Kagawa MD
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  8. Kei Kashima MD
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Itoh, Y., Okanoue, T., Enjo, F. et al. Regulation of hepatocyte albumin and α1-acid glycoprotein secretion by monokines, dexamethasone, and nitric oxide synthase pathway: Significance of activated liver nonparenchymal cells. Digest Dis Sci 39, 851–860 (1994). https://doi.org/10.1007/BF02087433

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  • DOI: https://doi.org/10.1007/BF02087433

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