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, Volume 2, Issue 5, pp 246–256 | Cite as

Alterations in45Ca distribution and movements in ileal longitudinal smooth muscle

  • George B. Weiss
Article

Abstract

The distribution and movements of45Ca were examined in longitudinal smooth muscle isolated from guinea-pig ileum. Alterations in45Ca distribution were induced by lack of equilibration prior to45Ca incubation, decreased temperature, iodoacetic acid (IAA), decreased nonradioactive Ca++ (increased specific activity of45Ca), and increased K+, but not by acetylcholine, histamine or carbachol. Efflux of45Ca was observed as two components with half-times of about 1 and 9.5 minutes. Either substitution of K+ for Na+ or addition of 80 mM K+ to O−Ca bathing solutions decreased the washout of45Ca in a sustained manner, whereas addition of nonradioactive Ca++ elicited both transient and maintained increases. Transient effects indicate a displacement of bound45Ca; maintained increases could be attributed to a decreased reuptake of45Ca. The K+-induced decrease in45Ca efflux may result from a decreased cellular pool of available45Ca after an inward shift of bound Ca++. Absence of similar changes in45Ca fluxes after exposure to stimulatory agents indicates that intracellular release and rebinding of Ca++ may oscur without detectable changes in cellular Ca++ or45Ca fluxes.

Keywords

Smooth Muscle Histamine Acetylcholine Ileal Detectable Change 

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Copyright information

© Birkhäuser Verlag 1972

Authors and Affiliations

  • George B. Weiss
    • 1
    • 2
  1. 1.Department of Pharmacology, Medical College of VirginiaVirginia Commonwealth UniversityRichmond
  2. 2.Department of PharmacologyUniversity of Texas Southwestern Medical SchoolDallas

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