Agents and Actions

, Volume 2, Issue 5, pp 246–256 | Cite as

Alterations in45Ca distribution and movements in ileal longitudinal smooth muscle

  • George B. Weiss


The distribution and movements of45Ca were examined in longitudinal smooth muscle isolated from guinea-pig ileum. Alterations in45Ca distribution were induced by lack of equilibration prior to45Ca incubation, decreased temperature, iodoacetic acid (IAA), decreased nonradioactive Ca++ (increased specific activity of45Ca), and increased K+, but not by acetylcholine, histamine or carbachol. Efflux of45Ca was observed as two components with half-times of about 1 and 9.5 minutes. Either substitution of K+ for Na+ or addition of 80 mM K+ to O−Ca bathing solutions decreased the washout of45Ca in a sustained manner, whereas addition of nonradioactive Ca++ elicited both transient and maintained increases. Transient effects indicate a displacement of bound45Ca; maintained increases could be attributed to a decreased reuptake of45Ca. The K+-induced decrease in45Ca efflux may result from a decreased cellular pool of available45Ca after an inward shift of bound Ca++. Absence of similar changes in45Ca fluxes after exposure to stimulatory agents indicates that intracellular release and rebinding of Ca++ may oscur without detectable changes in cellular Ca++ or45Ca fluxes.


Smooth Muscle Histamine Acetylcholine Ileal Detectable Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    A.M. Shanes,Electrochemical Aspects of Physiological and Pharmacological Action in Excitable Cells. Part II. The Action Potential and Excitation, Pharmac. Rev.10, 165–273 (1958).Google Scholar
  2. [2]
    A. Sandow,Excitation-Contraction Coupling in Skeletal Muscle, Pharmac. Rev.17, 265–320 (1965).Google Scholar
  3. [3]
    W.G. Nayler,Influx and Efflux of Calcium in the Physiology of Muscle Contraction, Clin. Orthop. Rel. Res.46, 157–182 (1966).Google Scholar
  4. [4]
    E.E. Daniel,Attempted Synthesis of Data Regarding Divalent Ions in Muscle Function, in:Muscle (Eds. W.M. Paul, E.E. Daniel, C.M. Cay, and G. Monckton; Pergamon, London 1965), p. 295–313.Google Scholar
  5. [5]
    H. Lüllmann,Calcium Fluxes and Calcium Distribution in Smooth Muscle, in:Smooth Muscle (Eds. E. Bülbring, A.F. Brading, A.W. Jones and T. Tomita; Williams and Wilkins, Baltimore 1970), p. 151–165.Google Scholar
  6. [6]
    L. Hurwitz, B. Tinsley andF. Battle,Dissociation of Contraction and Potassium Efflux in Smooth Muscle, Am. J. Physiol.199, 107–111 (1960).Google Scholar
  7. [7]
    K.A.P. Edman andH.O. Schild,The Need for Calcium in the Contractile Responses Induced by Acetylcholine and Potassium in the Rat Uterus, J. Physiol. Lond.161, 424–441 (1962).Google Scholar
  8. [8]
    D.F. Bohr,Electrolytes and Smooth Muscle Contraction, Pharmac. Rev.16, 85–111 (1964).Google Scholar
  9. [9]
    D.H.L. Evans, H.O. Schild andS. Thesleff,Effects of Drugs on Depolarized Plain Muscle, J. Physiol., Lond.143, 474–485 (1958).Google Scholar
  10. [10]
    G.B. Weiss andL. Hurwitz,Physiological Evidence for Multiple Calcium Sites in Smooth Muscle, J. gen. Physiol.47, 173–187 (1963).Google Scholar
  11. [11]
    C. van Breemen andE.E. Daniel,The Influence of High Potassium Depolarization and Acetylcholine on Calcium Exchange in the Rat Uterus, J. gen. Physiol.49, 1299–1317 (1966).Google Scholar
  12. [12]
    H. Lüllmann andA. Siegfriedt,Über den Calciumgehalt und den 45 Calciumaustausch in Längsmuskulatur des Meerschweinchendünndarms, Pflügers Arch. ges. Physiol.300, 108–119 (1968).Google Scholar
  13. [13]
    C.P. Bianchi,Cell Calcium (Butterworth, London 1968).Google Scholar
  14. [14]
    G.B. Weiss, R.E. Coalson andL. Hurwitz,K-Transport and Mechanical Responses of Isolated Longitudinal Smooth Muscle from Guinea-Pig Ileum, Am. J. Physiol.200, 789–793 (1961).Google Scholar
  15. [15]
    W.D.M. Paton andA.M. Rothschild,The Changes in Response and in Ionic Content of Smooth Muscle Produced by Acetylcholine Action and by Calcium Deficiency, Br. J. Pharmac. Chemother.24, 437–448 (1965).Google Scholar
  16. [16]
    G.B. Weiss,Alterations of 22 Na Distribution in Ileal Smooth Muscle, Am. J. Physiol.217, 828–834 (1969).Google Scholar
  17. [17]
    G.B. Weiss,Effects of Potassium, Acetylcholine, Carbachol and Histamine on Na 22 Movements in Ileal Smooth Muscle, J. Pharmac. exp. Ther.170, 123–131 (1969).Google Scholar
  18. [18]
    G.B. Weiss andF.R. Goodman,Effects of Lanthanum on Contraction, Calcium Distribution and Ca 45 Movements in Intestinal Smooth Muscle, J. Pharmac. exp. Ther.169, 46–55 (1969).Google Scholar
  19. [19]
    A.M. Shanes andC.P. Bianchi,The Distribution and Kinetics of Release of Radiocalcium in Tendon and Skeletal Muscle, J. gen. Physiol.42, 1123–1137 (1959).Google Scholar
  20. [20]
    C.P. Bianchi,The Effect of EDTA and SCN on Radiocalcium Movement in Frog Rectus Abdominis Muscle during Contractures Induced by Calcium Removal, J. Pharmac. exp. Ther.147, 360–370 (1965).Google Scholar
  21. [21]
    G.B. Weiss,The Effects of Potassium on Nicotine-Induced Contracture and Ca 45 Movements in Frog Sartorius Muscle, J. Pharmac. exp. Ther.154, 595–604 (1966).Google Scholar
  22. [22]
    G.B. Weiss,Homogeneity of Extracellular Space Measurement in Smooth Muscle, Am. J. Physiol.210, 771–776 (1966).Google Scholar
  23. [23]
    I.M. Glynn,The Action of Cardiac Glycosides on Ion Movements, Pharmac. Rev.16, 381–407 (1964).Google Scholar
  24. [24]
    R. Casteels,The Action of Ouabain on the Smooth Muscle Cellsof the Guinea-Pig's Taenia Coli, J. Physiol., Lond.184, 131–142 (1966).Google Scholar
  25. [25]
    G.B. Weiss,Dependence of Nicotine-C 14 Distribution and Movements upon pH in Frog Sartorius Muscle, J. Pharmac. exp. Ther.160, 135–147 (1968).Google Scholar
  26. [26]
    G. Burnstock andR.W. Straub,A Method for Studying the Effects of Ions and Drugs on the Resting and Action Potentials in Smooth Muscle with External Electrodes, J. Physiol., Lond.140, 156–167 (1958).Google Scholar
  27. [27]
    R.P. Durbin andD.H. Jenkinson,The Effect of Carbachol on the Permeability of Depolarized Smooth Muscle to Inorganic Ions, J. Physiol., Lond.157, 74–89 (1961).Google Scholar
  28. [28]
    C.P. Bianchi andA.M. Shanes,The Effect of the Ionic Milieu on the Emergence of Radiocalcium from Tendon and from Sartorius Muscle, J. cell. comp. Physiol.56, 67–76 (1960).Google Scholar
  29. [29]
    C. van Breemen, E.E. Daniel andD. van Breemen,Calcium Distribution and Exchange in the Rat Uterus, J. gen. Physiol.49, 1265–1297 (1966).Google Scholar
  30. [30]
    I. Krejci andE.E. Daniel,Effects of Altered External Calcium Concentrations on Fluxes of Calcium 45 in Rat Myometrium, Am. J. Physiol.219, 263–269 (1970).Google Scholar
  31. [31]
    A.H. Briggs,Calcium Movements during Potassium Contracture in Isolated Rabbit Aortic Strips, Am. J. Physiol.203, 849–852 (1962).Google Scholar
  32. [32]
    P.M. Hudgins andG.B. Weiss,Characteristics of 45 Ca Binding in Vascular Smooth Muscle, Am. J. Physiol.217, 1310–1315 (1969).Google Scholar
  33. [33]
    H.J. Schatzmann,Calciumaufnahme und-abgabe am Darmmuskel des Meerschweinchens, Pflügers Arch. ges. Physiol.274, 295–310 (1961).Google Scholar
  34. [34]
    F.R. Goodman andG.B. Weiss,Dissociation by Lanthanum of Smooth Muscle Responses to Potassium and Acetylcholine, Am. J. Physiol.220, 759–766 (1971).Google Scholar
  35. [35]
    F.R. Goodman andG.B. Weiss,Effects of Lanthanum on 45 Ca Movements and on Contractions Induced by Norepinephrine, Histamine and Potassium in Vascular Smooth Muscle, J. Pharmac. exp. Ther.177, 415–425 (1971).Google Scholar
  36. [36]
    L. Hurwitz, F. Battle andG.B. Weiss,Action of the Calcium Antagonists Cocaine und Ethanol on Contraction and Potassium Efflux of Smooth Muscle, J. gen. Physiol.46, 315–332 (1962).Google Scholar
  37. [37]
    G.B. Weiss andC.P. Bianchi,The Effect of Potassium Concentration on Ca 45 Uptake in Frog Sartorius Muscle, J. cell. comp. Physiol.65, 385–392 (1965).Google Scholar
  38. [38]
    N. Jenerick,Muscle Membrane Potential, Resistance and External Potassium Chloride, J. cell. comp. Physiol.42, 427–448 (1953).Google Scholar
  39. [39]
    N. Urakawa andW.C. Holland,Ca 45 Uptake and Tissue Ca in K-induced Phasic and Tonic Contraction in Taenia Coli, Am. J. Physiol.207, 873–876 (1964).Google Scholar
  40. [40]
    I. Krejci andE.E. Daniel,Effect of Contraction on Movements of Calcium 45 into and out of Rat Myometrium, Am. J. Physiol.219, 256–262 (1970).Google Scholar
  41. [41]
    T. Nasu, H. Karaki, M. Ikeda andN. Urakawa,Effect of Histamine on Calcium Exchange in Guinea-Pig Taenia Coli, Jap. J. Pharmac.20, 603–605 (1970).Google Scholar
  42. [42]
    A.P. Somlyo andA.V. Somlyo,Vascular Smooth Muscle. I. Normal Structure, Pathology, Biochemistry and Biophysics, Pharmac. Rev.20, 197–272 (1968).Google Scholar
  43. [43]
    A.M. Shanes,Movement of Calcium in Muscle and Nerve, in:The Transfer of Calcium and Strontium across Biological Membranes (Ed. R.H. Wasserman; Academic, New York 1963), p. 359–374.Google Scholar
  44. [44]
    G. Gabella,Caveolae Intracellulares and Sarcoplasmic Reticulum in Smooth Muscle, J. cell. Sci.8, 601–609 (1971).Google Scholar

Copyright information

© Birkhäuser Verlag 1972

Authors and Affiliations

  • George B. Weiss
    • 1
    • 2
  1. 1.Department of Pharmacology, Medical College of VirginiaVirginia Commonwealth UniversityRichmond
  2. 2.Department of PharmacologyUniversity of Texas Southwestern Medical SchoolDallas

Personalised recommendations