Summary
The affinity between purified rheumatoid factors (RF) and native or heat aggregated human IgG has been studied in vitro by polarization florescence in the presence and in the absence of D-penicillamine. The value of the dissociation constant was the same using native and heat aggregated IgG suggesting that binding to the aggregated protein is not dependent on the exposure of a new determinant lacking in the native molecule. The results obtained in the presence of D-penicillamine suggest that the concentration of the drug necessary to get a pronounced effect on the apparent dissociation constant of the immunocomplex between IgG and RF is not reached in vivo, in clinical situations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
I. Mohammed, D. Barraclough, E.J. Holborow and B.M. Ansell, Ann. rheum. Dis.33, 458 (1976).
I.A. Jaffe, Arthritis Rheum.13, 436 (1970).
E. Jellum and S. Skrede, in: Penicillamine Research in Rheumatoid Disease, p. 68. Ed. E. Munthe. Fabritius, MSD, 1977.
H. Deicher, U. Piening, M. Fricke, H. Von Wilmowsky and P.C. Fink, in: Protides of the Biological Fluids, p. 183. Ed. H. Peeters. Pergamon Press, Oxford and London 1979.
G. Mancini, A.D. Carbonara and J.F. Heremans, Int. J. Immunochem.2, 235 (1965).
D.E. Normansell, Immunochemistry8, 593 (1971).
S. Dissanayake, F.C. Hay and I.M. Roitt, Immunology32, 309 (1977).
E.J. Holborow and G.D. Johnson, in: Handbook of Experimental Immunology, p. 571. Ed. D.M. Weir. Blackwell Scientific Publications, 1967.
B.J. Davis, Ann. N. Y. Acad. Sci.121, 404 (1964).
J.E. Churchich, Biochim. biophys. Acta147, 511 (1967).
J. Ovadi, C. Salerno, T. Keleti and P. Fasella, Eur. J. Biochem.90, 499 (1978).
R. Saetre and D.L. Rabenstein, Analyt. Chem.50, 276 (1978).
J.V. Wells, in: Basic and Clinical Immunology, p. 225. Ed. H.H. Fudenberg, D.P. Stites, J.L. Caldwell and J.V. Wells. Lange Medical Publications, 1976.
L.A. Vanes, M.R. Daha and A. Kijlstra, in: Protides of Biological Fluids, p. 159. Ed. H. Peeters. Pergamon Press, Oxford and London 1979.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Giacomello, A., Salerno, C. & Gigante, M.C. Action of D-penicillamine on immunocomplexes containing rheumatoid factor. Experientia 37, 1070–1071 (1981). https://doi.org/10.1007/BF02085013
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02085013