Summary
Anti actin Fab-fragments were tagged to a small electron dense tracer molecule; ferrocene monocarboxylic acid (230 daltons). The conjugate stains actin filaments, which were found mainly in the core of microvilli.
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Literatur
K.T. Tokuyasu and S.J. Singer, J. Cell Biol.71, 894 (1976).
D. Drenckhahn and U. Gröschel-Stewart, J. Cell Biol.86, 475 (1980).
P.M. Elias, J. Goerke and D.S. Friend, J. Cell Biol.78, 577 (1977).
J. Ohtsuki, R.M. Manzi, G.E. Palade and J.D. Jamieson. Biol. cell.31, 119 (1978).
W.D. Kuhlmann, S. Avrameas and T. Ternynck, Immunol. Meth.5, 33 (1974).
E. Lazarides and K. Weber, Proc. natl Acad. Sci. USA71, 2268 (1974).
K. Weber, R. Koch, W. Herzog and J. Vandekerckhove, Eur. J. Biochem.78, 27 (1977).
E. Lazarides and U. Lindberg, Proc. natl Acad. Sci. USA71, 4742 (1974).
D.M. Livingston, Meth. Enzymol.34, 723 (1974).
R.R. Porter, Biochem. J.73, 119 (1959).
J.C. Sheehan and G.P. Hess, J. Am. chem. Soc.77, 1067 (1955).
L. Towbin, T. Staehelin and J. Gordon, Proc. natl Acad. Sci. USA76, 4350 (1979).
M. Osborn and K. Weber, Cell12, 561 (1977).
J.N. Dumont and M.V. Cone, Stain Technol.45, 188 (1970).
B.W. Lubit and J.H. Schwartz, J. Cell Biol.86, 891 (1980).
E. Karsenti, B. Guilbert, M. Bornes, S. Avrameas, R. Whalen and D. Pantaloni, J. Histochem. Cytochem.26, 934 (1978).
W. Bohn, J. Histochem. Cytochem.26, 293 (1978).
J. Roth, M. Bendayan and L. Orci, J. Histochem. Cytochem.26, 1074 (1978).
J.P. Kraehenbuhl, R.E. Galardy and J.D. Jamieson, J. exp. Med.139, 208 (1974).
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Tiggemann, R., Govindan, M.V. A low molecular weight tracer molecule for immunocytochemistry. Identification of cytoplasmic actin. Experientia 37, 1066–1068 (1981). https://doi.org/10.1007/BF02085011
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DOI: https://doi.org/10.1007/BF02085011