Skip to main content
SpringerLink
Log in

Kinetics of hydroperoxyde-dependent naphthalene hydroxylation involving cytochrome P-450 of the intact and induced enzyme systems of rat liver microsomes

  • Published:
Reaction Kinetics and Catalysis Letters Aims and scope Submit manuscript

Abstract

Hydroperoxide-dependent naphthalene hydroxylation involving rat liver microsomes has been studied. The direct interaction between cytochrome p-450 and cumyl hydroperoxide (CHP) has been demonstrated. Km values with respect to both the substrate and CHP have been determined using intact and induced enzyme systems. The nature of the hydroxylating agent and the possible role of high-valence iron in the processes of hydroxylation by microsomal systems is discussed.

Abstract

Показано гидроксилилирование нафталина гидроперекисыо кумила ГПК. Показано непосредственное взаимодействие цитохрома P-450 с ГПК. Определены величины Km по субстрату и по ГПК при использовании интактных и индуцированных ферментных систем. Обсуждается природа гидроксилирующей частицы, а также возможная роль высших состояний железа в процессах гидроксилирования микросомальными системами.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. D. Rahimtula, P. J. O'Brien: biochem. Biophys. Res. Commun.,60, 440 (1974).

    PubMed  Google Scholar 

  2. A. A. Akhrem, M. L. Abeliovich, S. A. Usanov, S. S. Dvornikov, D. I. Metelitsa: Bioorg. Khim.,2, 549 (1976).

    Google Scholar 

  3. W. R. Bidlack, P. Hofstein: Life Sci.,14, 2003 (1974).

    PubMed  Google Scholar 

  4. E. G. Hrycay, P. J. O'Brien: Arch. Biochem. Biophys.,160, 230 (1974).

    PubMed  Google Scholar 

  5. A. A. Akhrem, S. A. Usanov, D. I. Metelitsa: Dokl. Akad. Nauk SSSR,218, 1457 (1974).

    PubMed  Google Scholar 

  6. A. A. Akhrem, S. A. Usanov, S. S. Dvornikov, D. I. Metelitza: Dokl. Akad. Nauk SSSR,223, 1014 (1975).

    PubMed  Google Scholar 

  7. T. Omura, R. Sato: J. Biol. Chem.,239, 2370 (1974).

    Google Scholar 

  8. E. G. Hrycay, P. J. O'Brien: Arch. Biochem. Biophys.,157, 7 (1973).

    PubMed  Google Scholar 

  9. J. Yamazaki, K. Yokota: Mol. Cell Biochem.,2, 39 (1973).

    PubMed  Google Scholar 

  10. V. P. Beriya, V. E. Kagan, Y. E. Arkhipenko, Y. P. Kozlov: Biofizika,20, 238 (1975).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Bioorganic Chemistry, Belorussian Academy of Sciences, Minsk, USSR

    A. A. Akhrem, S. A. Usanov, S. S. Dvornikov & D. I. Metelitsa

Authors
  1. A. A. Akhrem
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. A. Usanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. S. Dvornikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. I. Metelitsa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Akhrem, A.A., Usanov, S.A., Dvornikov, S.S. et al. Kinetics of hydroperoxyde-dependent naphthalene hydroxylation involving cytochrome P-450 of the intact and induced enzyme systems of rat liver microsomes. React Kinet Catal Lett 6, 187–194 (1977). https://doi.org/10.1007/BF02084196

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02084196

Keywords

Search

Navigation