Journal of tissue culture methods

, Volume 6, Issue 3–4, pp 107–112 | Cite as

Calculator programmed assistance in making cell cultures

  • J. C. Bullaro


A calculator program and instructions are presented for facilitating the preparation of single cell suspensions to be used in cell culture systems. The program has been written for the Texas Instruments Inc. TI-59 programmable calculator with printer. The instructions provided are intended for the novice with no previous programming experience and only a basic understanding of the calculator operation. The program provides the appropriate volume and cell concentration of the inoculum cell suspension for a given set of culture parameters. It also provides options for culture initiation when the cell yield in the concentrated suspension is insufficient to satisfy the parameters selected.

Key words

cell culture cell suspension calculator programming computer 


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V. References

  1. 1.
    Personal programming. A complete owner's manual for TI Programmable 58/59. Texas Instruments Inc., Dallas, Texas. 1977.Google Scholar
  2. 2.
    Bullaro, J. C.; Brookman, D. H. Comparison of skeletal muscle monolayer cultures initiated with cells dissociated by the vortex and trypsin methods. In Vitro 12: 564–570; 1976.PubMedGoogle Scholar
  3. 3.
    Bullaro, J. C. Technique for measuring picomolar amounts of bound and unbound amino acids obtained from myogenic cell cultures of skeletal muscle. In Vitro. In press.Google Scholar

Copyright information

© Tissue Culture Association, Inc 1981

Authors and Affiliations

  • J. C. Bullaro
    • 1
  1. 1.Department of Anatomy College of MedicineUniversity of SaskatchewanSaskatoonCanada

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