Skip to main content
SpringerLink
Log in

Effects of chronic renal denervation in conscious restrained rats

  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

Abstract

Sodium (Na), calcium (Ca), inorganic phosphate (Pi) and water excretion were measured in nondiuretic (ND) and extracellular fluid (ECF) volume expanded (VE) conscious restrained rats four weeks after denervation or sham-denervation of the left kidney. On the day of the study the animals were lightly anaesthetized with ether and the femoral vessels on one side were catheterized. Urine was collected from both kidneys. The animals were allowed to recover for 3 hours and studied in a restraining chamber. In ND animals isotonic saline containing inulin and para-amino-hippuric acid (PAH) were given at a rate of 0.067±0.002 (SE) ml/min/kg body weight (BW). In VE animals the infusion rate was 0.24±0.04 ml/min/kg BW. Kidney catecholamine content was measured after the experiments. Clearances of PAH and of inulin (GFR) were the same in both kidneys. Urine volume (V), sodium excretion (UNaV/GFR), inorganic phosphate excretion (UPiV/GFR) and calcium excretion (UCaV/GFR) were significantly higher in the denervated kidneys. Values in sham denervated kidneys were not greater than those of the right kidney. Denervation was proven by demonstrating absent or very low catecholamine content in the kidneys.

The results demonstrate that: (a) chronic renal denervation in rats leads to diuresis and natriuresis even in the conscious state, thus confirming previous results from our laboratory; (b) such changes occur independently of the state of the ECF volume and of renal haemodynamic changes; (c) the increased excretion of Ca++ and Pi after denervation demonstrates that renal nerves affect the reabsorption of these ions either independently or by way of their effect on sodium reabsorption. These data allow us to suggest that a renal tubular dysfunction, which was proved in anaesthetized denervated animals, can also be observed in the conscious state.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. DiBona, G. F.: Neurogenic regulation of renal tubular sodium reabsorption.Am. J. Physiol, 233, 73 (1977).

    Google Scholar 

  2. Takács, L., Bencsáth, P., Szalay, L.,: Decreased proximal tubular transport capacity after renal sympathectomy. Proc. 7th Int. Congr. Nephrol., Montreal, 1978, pp. 553–558.

  3. Gottschalk, C. W.: Renal nerves and sodium excretion.Annu. Rev. Physiol., 41, 229 (1979).

    PubMed  Google Scholar 

  4. Szalay, L., Bencsáth, P., Takács L.: Impaired proximal tubular transport functions in anesthetized splanchnicotomized dogs.Experientia, 33, 42 (1977).

    PubMed  Google Scholar 

  5. Szalay, L., Colindres, R. E., Jackson, R., Adkinson, J. T., Lassiter, W. E., Gottschalk, C. W.: Phosphate (Pi) transport in chronically denervated rat kidneys (Under publication).

  6. Szénási, G., Bencsáth, P., Lehoczky, E., Takács, L.: Tubular transport and urinary excretion of phosphate after renal denervation in the anesthetized rat.Am. J. Physiol., 240, 481 (1981).

    Google Scholar 

  7. Surtshin, A., Schmandt, W. P.: Comparison of continuously collected urines from the two normal kidneys and some effects of unilateral denervation.Am. J. Physiol., 185, 418 (1956).

    PubMed  Google Scholar 

  8. Berne, R. M.: Hemodynamics and sodium excretion of denervated kidney in anesthetized and unanesthetized dog.Am. J. Physiol., 171, 148 (1952).

    PubMed  Google Scholar 

  9. Smith, H. W.: The Kidney: Structure and Function in Health and Disease. New York-Oxford 1951, pp. 411–460.

  10. Lifschitz, M. D.: Lack of a role for the renal nerves in renal sodium reabsorption in conscious dogs.Clin. Sci. Mol. Med., 54, 567 (1978).

    PubMed  Google Scholar 

  11. Sadowski, J., Kurkus, J., Gellert, R.: Reinvestigation of denervation diuresis and natriuresis in conscious dogs.Arch. Int. Physiol. Biochim., 87, 663 (1979).

    PubMed  Google Scholar 

  12. Sadowski, J., Kurkus, J., Gellert, R.: Denervated and intact kidney responses to saline load in awake and anesthetized dogs.Am. J. Physiol., 237, 262 (1979).

    Google Scholar 

  13. Sadowski, J., Kurkus, J.: A search for a defect of proximal transport in denervated kidneys of conscious dogs.Arch. Int. Physiol. Biochim., 89, 149 (1981).

    PubMed  Google Scholar 

  14. Rogenes, P. R., Gottschalk, C. W.: Renal function in conscious rats with chronic unilateral renal denervation.Am. J. Physiol., 242 140 (1982).

    Google Scholar 

  15. Walsh, G. M., Ferrone, R. A.: Ether-induced vasodilation in the rat.Res. Commun. Chem. Pathol. Pharmacol., 24, 67 (1979).

    PubMed  Google Scholar 

  16. Coyle, J. T., Henry, D.: Catecholamines in fetal and newborn rat brain.J. Neurochem., 21, 61 (1973).

    PubMed  Google Scholar 

  17. Davidson, W. D., Sackner, M. A.: Simplification of the anthrone method for the determination of inulin in clearance studies.J. Lab. Clin. Med., 62, 351 (1963).

    PubMed  Google Scholar 

  18. Bratton, A. C., Marshall, E. K. Jr.: A new coupling component for sulfanilamide determination.J. Biol. Chem., 128, 537 (1939).

    Google Scholar 

  19. Chen, P. S., Jr., Toribara, T. Y., Warner, H.: Microdetermination of phosphorus.J. Anal. Chem., 28, 1756 (1956).

    Google Scholar 

  20. Hill, J. B.: Automated fluorometric method for determination of serum calcium.Clin. Chem., 11, 122 (1965).

    PubMed  Google Scholar 

  21. Pastoriza-Munoz, E., Colindres, R. E., Lassiter, W. E., Lechene, C.: Effect of parathyroid hormone on phosphate reabsorption in rat distal convolution.Am. J. Physiol., 235, 321 (1978).

    Google Scholar 

  22. Lassiter, W. E., Gottschalk, C. W., Mylle, M.: Micropuncture study of renal tubular reabsorption of calcium in normal rodents.Am. J. Physiol., 204, 771 (1963).

    Google Scholar 

  23. Gitelman, H. J., Kukolj, S., Welt, L. G.: The influence of the parathyroid glands on the hypercalcemia of experimental magnesium depletion in the rat.J. Clin. Invest., 47, 118 (1968).

    Google Scholar 

  24. LeGrimellec, Ch., Poujeol, P., deRouffignac, C.:3H-inulin and electrolyte concentrations in Bowman's capsule in rat kidney. Comparison with artificial ultrafiltration.Pfluegers Arch., 354, 117 (1975).

    Google Scholar 

  25. Bernstein, D. S., Aliapoulios, M. A., Hattner, R. S., Wachman, A., Rose, B.: Serum calcium ion activity: effects of thyrocalcitonin and parathyroid extract in the rat.Endocrinology, 85, 589 (1969).

    PubMed  Google Scholar 

  26. Sutton, R. A., Dirks, J. H.: The renal excretion of calcium: a review of micropuncture data.Can. J. Physiol. Pharmacol., 53, 979 (1975).

    PubMed  Google Scholar 

  27. de Takats, G., McDonald, G. O., Harridge, W. M.: The vicious circle in hypertension.Surgery, 43, 113 (1958).

    PubMed  Google Scholar 

  28. de Takats, G.: Sympathectomy for hypertension.Am. J. Sur. 127, 521 (1974).

    Google Scholar 

  29. Hix, E. L.: A new approach to ureteral exteriorization for bilateral renal studies.J. Appl. Physiol., 8, 114 (1955).

    PubMed  Google Scholar 

  30. Hix, E. L.: Uretero-renal reflex facilitating renal vasoconstrictor responses to emotional stress.Am. J. Physiol., 192, 191 (1958).

    PubMed  Google Scholar 

  31. Szalay, L., Bencsáth, P., Takács, L.: Effect of splanchnicotomy on the renal excretion of inorganic phosphate in the anesthetized dog.Pfluegers Arch., 367, 283 (1977).

    Google Scholar 

  32. Szalay, L., Takács, L.: Renal excretion of calcium and phosphate in normal and thyroparathyroidectomized dogs.Acta Physiol. Acad. Sci. Hung., 53, 216 (1979).

    Google Scholar 

  33. Pitts, R. F., Gurd, R. S., Kessler, R. H., Hierholzer, K.: Localization of acidification of urine, potassium and ammonia secretion and phosphate reabsorption in the nephron of the dog.Am. J. Physiol., 104, 125 (1958).

    Google Scholar 

  34. Wilde, W. S., Malvin, R. L.: Graphical placement of transport segments along the nephron from urine concentration pattern developed with stop flow technique.Am. J. Physiol., 195, 153 (1958).

    Google Scholar 

  35. Strickler, J. C., Thompson, D. D., Klose, R. M., Giebisch, G.: Micropuncture study of inorganic phosphate excretion in the rat.J. Clin. Invest., 43, 1596 (1964).

    PubMed  Google Scholar 

  36. Amiel, C., Kuntziger, H., Richet, G.: Micropuncture study of handling of phosphate by proximal and distal nephron in normal and parathyroidectomized rat. Evidence for distal reabsorption.Pfluegers Arch., 317, 93 (1970).

    Google Scholar 

  37. Bencsáth, P., Bonvalet, J. P., deRouffignac, C.: Tubular factors in denervation diuresis and natriuresis. In: H. Wirz, F. Spinelli (eds): Recent Advances in Renal Physiology. Karger, Basel 1972, pp. 96–106.

    Google Scholar 

  38. Bello-Reuss, E., Colindres, R. E., Pastoriza-Munoz, E., Mueller, R. A., Gottschalk, C. W.: Effects of acute unilateral renal denervation in the rat.J. Clin. Invest., 56, 208 (1975).

    PubMed  Google Scholar 

  39. Vaziri, D. N., Nellans, R. E., Brueggemann, R. M., Barton, C. H., Martin, D. C.: Renal tubular dysfunction in transplanted kidneys.South. Med. J., 72, 530 (1979).

    PubMed  Google Scholar 

  40. Norwell, J. E., Weitsen, H. A., Dwyer, J. J.: Degeneration and regeneration of adrenergic nerves in the autotransplanted kidney.Transplantation 7, 218 (1969).

    PubMed  Google Scholar 

  41. Norwell, J. E., Weitsen, H. A., Sheppek, C. G.: The intrinsic innervation of human renal homotransplants.Transplantation, 9, 168 (1970).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Physiology and Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

    R. E. Colindres, R. Jackson & C. W. Gottschalk

  2. 2nd Department of Medicine, Semmelweis University Medical School, Budapest, Hungary

    L. Szalay

Authors
  1. L. Szalay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. E. Colindres
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Jackson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. W. Gottschalk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Szalay, L., Colindres, R.E., Jackson, R. et al. Effects of chronic renal denervation in conscious restrained rats. International Urology and Nephrology 18, 3–18 (1986). https://doi.org/10.1007/BF02082643

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02082643

Keywords

Search

Navigation