International Urology and Nephrology

, Volume 12, Issue 4, pp 367–374 | Cite as

The effect of uraemic “middle-sized molecules” on T lymphocyte functions

  • K. Kálmán
  • L. Lőcsey
  • M. Hauck
  • A. Leövey
Article

Abstract

The activity of the leucocyte inhibitory factor (LIF) stimulated with Concanavalin A was examined in chronic uraemic, haemodialysed patients. It was found that the LIF activity decreased in chronic uraemic patients as compared with the normal controls.

The absolute T lymphocyte count and active E rosette formation also decreased. Inin vitro experiments the authors have also examined the inhibitory effect of the socalled “middle-sized molecule” (MSM) on uraemic materials separated from the serum and the haemofiltrate. They observed that the materials with a molecular weight of 1000–1500 isolated both from the serum and the haemofiltrate significantly inhibited the Con A stimulated LIF production of normal lymphocytes and decreased the ratio of the active E rosette-forming T lymphocytes.

On the basis of their exeeriments the MSM uraemic materials are considered responsible for the decreased immune reactivity of uraemic patients. These uraemic toxins can be dialysed.

Keywords

Public Health Molecular Weight Immune Reactivity Normal Control Inhibitory Factor 

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References

  1. 1.
    Bendtzen, K.: Inhibition of human migration inhibitory factor (LIF) by a-L-fucose.Acta Allerg., 30, 327 (1975).Google Scholar
  2. 2.
    Bergström, J., Asaba, H., Fürst, P., Oules, R.: Dialysis, ultrafiltration and blood pressure,Proc. Eur. Trans. Assoc., 13, 293 (1976).Google Scholar
  3. 3.
    Bergström, J., Fürst, P.: Uremic middle molecules.Clin. Nephrol., 5, 143 (1975).Google Scholar
  4. 4.
    Birkeland, A. A.: Uremia as a state of immune deficiency.Scand. J. Immunol., 5, 107 (1976).PubMedGoogle Scholar
  5. 5.
    Felsburger, J., Edelman, J., Gilman, R. H.: The active E rosette test: correlation with delayed hypersensitivity.J. Immunol., 116, 1110 (1976).Google Scholar
  6. 6.
    Gorski, A. J., Dupont, B., Hansen, J. A.: Leucocyte migration inhibitory factor (LMIF) profile in primary and secondary immune deficiency disease.Clin. Exp. Immunol., 26, 505 (1976).Google Scholar
  7. 7.
    Harris, J. E., Page, D., Posen, G., Stewart, T.: Suppression of in vitro lymphocyte function by uremic toxins.J. Urol., 108, 312 (1972).PubMedGoogle Scholar
  8. 8.
    Harris, J., Sengar, U. D.: Immune deficiency in chronic uraemia. Preliminary evidence for thymosin deficiency.Transplantatio, 20, 176 (1975).Google Scholar
  9. 9.
    Hoy, W. E., Cestero, R. V. M., Freeman, R. B.: Deficiency of T and B lymphocytes in uremic subjects and partial improvement with maintenance hemodialysis.Nephron, 20, 182 (1978).PubMedGoogle Scholar
  10. 10.
    Jondal, M., Holm, G., Wigzell, M.: Surface markers on human T and B lymphocytes.J. Exp. Med., 136, 207 (1972).PubMedGoogle Scholar
  11. 11.
    Kauffman, C. A., Manzler, A. D., Phair, J. P.: Cell-mediated immunity in patients on long term hemodialysis.Clin. Exp. Immunol., 22, 54 (1975).PubMedGoogle Scholar
  12. 12.
    Montgomerie, J. Z., Kalmanson, G. M., Guze, L. B.: Renal failure and infection.Medicine (Baltimore),47, 1 (1968).Google Scholar
  13. 13.
    Nelson, D. S., Penrose, J. M.: Effect of hemodialysis and transplantation on inhibition of lymphocyte transformation by sera from uremic patients.Clin. Immunol. Immunopathol., 4, 143 (1975).PubMedGoogle Scholar
  14. 14.
    Newberry, W. M., Sanford, J. P.: Defective cellular immunity in renal failure serum.J. Clin. Invest., 50, 1262 (1971).PubMedGoogle Scholar
  15. 15.
    Papadimitriou, M., Baker, L. R. J., Seitanidis, B., Sevitt, L. H., Hulatilake, A. E.: White blood count in patients on regular hemodialysis.Brit. Med. J., 4, 67 (1969).PubMedGoogle Scholar
  16. 16.
    Salvin, R. G., Fitsch, D. D.: Inhibition of lymphocyte transformation by guanidinoscuccinic acid, a surplus metabolite in uremia.Experientia, 27, 1340 (1971).PubMedGoogle Scholar
  17. 17.
    Scheurlein, P. G., Baake, M., Frey, N., Mores, P., Seiberth, G.: Über Immuninsuffizienz bei chronischen, urämischen Nierenerkrankungen.Dtsch. Med. Wsch., 94, 17 (1969).Google Scholar
  18. 18.
    Selroos, O., Paternach, A., Virolainen, M.: Skin test sensitivity and antigen induced lymphocyte transformation in uremia.Clin. Exp. Immunol., 14, 365 (1973).PubMedGoogle Scholar
  19. 19.
    Søborg, M., Bendixen, G.: Human leukocyte migration as parameter of hypersensitivity.Acta Med. Scand., 18, 247 (1967).Google Scholar
  20. 20.
    Szabó, G., Szegedi, Gy., Fekete, B., Petrányi, Gy.: Tuberculin sensitivity test, using leukocyte migration inhibition, in patients with autoimmune disease undergoing immunosuppressive therapy.Orv. Hetil., 114, 1914 (1973).PubMedGoogle Scholar
  21. 21.
    Touraine, J. B., Touraine, F., Revillard, J. P., Brochier, J., Traeger, J.: T lymphocytes and serum inhibitor of cell-mediated immunity in renal instufficiency.Nephron, 14, 195 (1975).PubMedGoogle Scholar
  22. 22.
    Wybran, J., Fudenberg, H.: Thymus derived rosette-forming cells in various human disease states: Cancer, lymphoma, bacterial and viral infections, and other diseases.J. Clin. Invest., 52, 1026 (1973).PubMedGoogle Scholar
  23. 23.
    Yu, D. T. Y.: Human lymphocyte subpopulation early and late rosettes.J. Immunol., 115 91 (1975).PubMedGoogle Scholar

Copyright information

© Akadämiai Kiadó 1980

Authors and Affiliations

  • K. Kálmán
    • 1
  • L. Lőcsey
    • 1
  • M. Hauck
    • 1
  • A. Leövey
    • 1
  1. 1.First Department of Medicine and Institute of BiochemistryUniversity Medical SchoolDebrecen

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