Abstract
A method of radioimmunologic quantitation of antibodies to streptococcal antigen separated from the cell wall extract of group A type T12 strain has been developed. The highest values of radioactive antigen binding were observed in acute glomerulonephritis (75%), as compared to chronic glomerulonephritis in which values of 25% to 56% were found depending on the morphology of renal changes. It was shown that none of the patients with pyelonephritis, Alport's syndrome, lupoid nephritis and polycystic renal disease had elevated antistreptococcal antibody levels. In contrast to this, all patients with tonsillitis and proteinuria exhibited increased titre of this antibody.
It was shown that the antigen is related neither to M-protein nor to group A polysaccharide and that it is not type-specific because the binding of antigen T12 may be inhibited by the antigen produced from strain T5. Although the antigen is not type-specific, some differences in the response to antigens prepared from various types of streptococci in patients with different form of chronic glomerulonephritis are observed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anthony, B. F.: Quantitative and qualitative studies of antibody to streptococcal M-protein.J. Immunol., 105, 379 (1970).
Beachey, E. H., Oftek, I., Cunningham, M., Bisno, A.: Evaluation of micro complement fixation tests for antibodies against group A streptococcal M and M-associated antigens in rabbit and human sera.Appl. Microbiol., 27, 1 (1974).
Berstain, S. H., Stillerman, M.: A study of the association of group A streptococci with acute glomerulonephritis.Ann. Intern. Med., 52, 1026 (1960).
Braun, D. G., Holm, S. E.: Streptococcal anti-group A precipitins in sera from patients with rheumatic fever and acute glomerulonephritis.Int. Arch. Allergy, 37, 216 (1970).
Dunlap, M. B., Bergin, J. W.: Maintenance of anti-M protein antibodies.Am. J. Publ. Health, 64, 916 (1974).
Freedman, P., Meister, H. P., Co, B. S., Markowitz, A. S., Dubin, A.: Subclinical response to streptococcal infection.New Engl. J. Med., 275, 795 (1966).
Grey, H. M.: Studies on the binding between streptococcal M protein and antibody.J. Exp. Med., 115, 671 (1962).
Mrzymański, M., Möller, E., Bergström, J.: Cell-mediated and humoral immunity to streptococcal cell wall antigenic extract in patients with glomerulonephritis and in healthy controls.Scand. J. Immunol., 4, 295 (1975).
Lancefield, R. C.: The antigenic complex of streptococcus haemolyticus. I. Demonstration of a type specific substance in extracts of streptococcus haemolyticus.J. Exp. Med., 47, 91 (1928).
Lange, K., Ahmed, U., Kleinberg, H., Treser, G.: A hitherto unknown streptococcal antigen and its probable relation to acute poststreptococcal glomerulonephritis.Clin. Nephrol., 5, 207 (1976).
McConahey, P. J., Dixon, F. J.: A method of trace iodination of proteins for immunologic studies.Int. Arch. Allergy Appl. Immunol., 29, 185 (1966).
Meadow, S. R.: Poststreptococcal glomerulonephritis — a rare disease?Arch. Dis. Child., 50, 379 (1975).
Pokora, J.: Antistreptolysins in renal diseases.Pol. Arch. Med. Wewn., 32, 81 (1962).
Quinn, R. W., Lowry, N. P.: Streptococcal M protein antibodies.J. Infect. Dis., 113, 33 (1963).
Widdowson, J. P., Maxted, W. R., Pinney, A. M.: An M-associated protein antigen (MAP) of group A streptococci.J. Hyg., 69, 555 (1971).
Zabriskie, J. B.: The role of streptococci in human glomerulonephritis.J. Exp. Med., 134, 180 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Krzymanski, M., Michalska, W. & Baczyk, K. Use of radioimmunoassay for the detection of circulating antistreptococcal antibody in patients with glomerulonephritis. International Urology and Nephrology 13, 299–307 (1981). https://doi.org/10.1007/BF02082429
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02082429