International Urology and Nephrology

, Volume 10, Issue 4, pp 335–347 | Cite as

Description and interpretation of functional changes in canine kidneys after preservation with hypothermic perfusates and subsequent reperfusion

  • E. Rőth
  • T. Szmolenszky
  • B. Török
Article

Abstract

The function of canine kidneys stored with hypothermic solutions (Rheomacrodex, Collins4, Sacks) was studied in acute autologous reperfusion models. Changes in the perfusates (increase in LDH level and minor increase in K+ level), as well as the quantitative and qualitative properties (proteinuria) of the urine produced by the graft may be ascribed to storage and revascularization which affect the cells and their membranes. The alterations of the circulatory parameters demonstrable at the outset and during revascularization are due to an increase in renovascular resistance of hypothermic origin on the one hand, and to acute tubular epithelial damage inherent in the procedure, on the other.

Keywords

Public Health Proteinuria Functional Change Qualitative Property Minor Increase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aboul-Enein, A., Paccione, F., Todd, I. A. D., Shikata, T., Dempster, W. J., Kountz, S. L.: A technique for successful 20 h kidney storage at 4°C.Experientia, 21, 546 (1965).PubMedGoogle Scholar
  2. 2.
    Abuna, G. J. M.: Viability assays in organ preservation. In: Organ Preservation for Transplantation. Little, Brown and Comp., Boston 1974, p. 108.Google Scholar
  3. 3.
    Arendshort, W. J., Finn, W. F., Gottschalk, C. W.: Pathogenesis of acute renal failure following temporary renal ischemia in the rat.Circ. Res., 37, 558 (1975).PubMedGoogle Scholar
  4. 4.
    Basso, A. G., Netto, C. V., Cockett, A. T. K.: Renal preservation with a modified intracellular hypothermic solution.Invest. Urol., 11, 136 (1973).PubMedGoogle Scholar
  5. 5.
    Balzer, F. O.: Role of preservation in clinical renal transplantation.Transplant. Proc., 3, 268 (1971).PubMedGoogle Scholar
  6. 6.
    Belzer, F. O., Downes, G. L.: Kidney. In: Organ Preservation for Transplantation. Little, Brown and Comp., Boston 1974, p. 298.Google Scholar
  7. 7.
    Benjamin, J. L., Sell, K. W.: Assessment of renal function during isolatedin vitro perfusion.Transplant. Proc., 3, 616 (1971).PubMedGoogle Scholar
  8. 8.
    Berkowitz, H. D., Miller, L. D., Itskovitz, H. D.: Renal function and the renin-angiotensin system in the isolated perfused kidney.Amer. J. Path., 213, 928 (1967).Google Scholar
  9. 9.
    Chapman, B. J., Munday, K. A., Willson, R. A., Withey, W. R.: Regional renal blood flows in the hypothermic rat and dog.J. Physiol., 251, 20 (1975).Google Scholar
  10. 10.
    Clark, E. A., Opelz, G., Mickey, M. R., Terasaki, P. I.: Evaluation of Belzer and Collins kidney-preservation methods.Lancet. 1, 361 (1973).Google Scholar
  11. 11.
    Collins, G. M., Bravo-Shugarman, M., Terasaki, P. I.: Kidhey preservation for transplantation. Initial perfusion and 30 hours'ice storage.Lancet, 2, 1219 (1969).PubMedGoogle Scholar
  12. 12.
    Collste, H.: Preservation of kidneys for transplantation. Experimental studies.Acta chir. scand. Suppl., 425, 8 (1972).Google Scholar
  13. 13.
    Dougherty, J. C., Panossian, A., Nehlsen, S. L., Torres, M., Veith, F. J.: Distribution of blood flow in isolated kidneys perfused with blood, plasma and crystalloids. In: J. C. Norman (ed.): Organ Perfusion and Preservation. Appleton-Century-Crofts, New York 1968, p. 821.Google Scholar
  14. 14.
    Halasz, N. A., Collins, G. M.: Forty-eight-hour kídney preservation. A comparison of flushing and ice storage with perfusion.Arch. Surg., 111, 175 (1976).PubMedGoogle Scholar
  15. 15.
    Hart, W. H., Manax, W. G.: Viability, function and transplantation of preserved canine kidneys.Transplant. Proc., 3, 630 (1971).PubMedGoogle Scholar
  16. 16.
    Humphries, A. L. Jr.: Problems with various perfusates for kidney preservation.Cryobiology, 5, 447 (1969).PubMedGoogle Scholar
  17. 17.
    Grundmann, R., Landes, T., Pichlmaier, H.: Hypothermic pulsatile perfusion of dog kidneys.Transplantation, 14, 242 (1972).Google Scholar
  18. 18.
    Itskovitz, H. D., Hebert, L. A., McGiff, J. C.: Angiotensin as a possible intrarenal hormone in isolated dog kidneys.Circ. Res., 32, 550 (1973).PubMedGoogle Scholar
  19. 19.
    Itskovitz, H. D., Terragno, N. A., McGiff, J. C.: PGE2 acts as an intrarenal hormone regulating medullary vascular resistance.J. clin. Invest., 52, 43a (1973).Google Scholar
  20. 20.
    Jensen, E. H., Kemp, E.: Kidney preservation. II. Liberation of enzymes and urea from preserved kidneys.Scand. J. Urol. Nephrol., 6, 280 (1972).PubMedGoogle Scholar
  21. 21.
    Jensen, E. H., Kemp, E.: Kidney preservation. III. The Importance of the composition of perfusion fluids in the transplantation of rabbit kidneys.Scand. J. Urol. Nephrol., 6, 284 (1972).PubMedGoogle Scholar
  22. 22.
    Kemp, E.: Kidney preservation. I. Evaluation of perfusion and ischemic damage.Scand. J. Urol. Nephrol., 6, 273 (1972).PubMedGoogle Scholar
  23. 23.
    Khuri, F. J., Panner, B., Linke, C. A., Cockett, T. K.: Comparison of Sacks II solution and modified Collins solution (Ursol) in simple cold preservation of canine kidneys.J. Surg. Res., 19, 361 (1975).PubMedGoogle Scholar
  24. 24.
    Liebau, G., Klose, H. J., Fischbach, H., Pichlmaier, H.: Simple tests for viability of the hypothermic pulsatile perfused dog kidney.Surgery, 70, 459 (1971).PubMedGoogle Scholar
  25. 25.
    Lonigro, A. J., Itskovitz, H. D., Crowshaw, K., McGiff, J. C.: Dependency of renal blood flow on prostaglandin synthesis in the dog.Circ. Res., 32 212 (1973).Google Scholar
  26. 26.
    Lotke, P. A., Schwartz, S. L.: Studies on the functional and biochemical changes during kidney preservation.Surgery, 67, 488 (1970).PubMedGoogle Scholar
  27. 27.
    McCabe, R., Applebaum, H., Lorieo, D., Stevens, L.: Failure of canine kidney preservation using Sacks II solution.Lancet, 1, 517 (1975).Google Scholar
  28. 28.
    Millner, J., Lackey, W. H.: Kidney preservation using combinations of Belzer and Collins techniques.Transplantation, 20, 278 (1975).PubMedGoogle Scholar
  29. 29.
    Monaco, A. P.: Transplantation.Surg. Gynec. Obstet., 140, 215 (1975).PubMedGoogle Scholar
  30. 30.
    Pausescu, E., Carnaru, S., Beroniade, V., Paunescu, V., Chirvasie, R.: Kidney preservation in a hypothermic “cytoplasmic” solution before transplantation. Ninth Conference on Dialysis and Renal Transplantation, 1972, p. 491.Google Scholar
  31. 31.
    Rőth, E., Tóth, I., Török, B.: Histological features of stored kidneys after simple hypothermic perfusion with preserving solutions of different types.Int. Urol. Nephrol., 6, 243 (1974).PubMedGoogle Scholar
  32. 32.
    Rőth, E., Szmolenszky, T., Török, B.: Funkcné a morfologické vysetrenia obliciek psov po prezervach hyperosmotickym roztokom.Bratisl. Lek. Listv. 65, 278 (1976).Google Scholar
  33. 33.
    Rőth, E., Deák, Gy., Szmolenszky, T.: Description and interpretation of structural changes in canine kidneys after preservation with a hyperosmolar perfusate and revascularization.Int. Urol. Nephrol. 9, 185 (1977).PubMedGoogle Scholar
  34. 34.
    Rudolf, L. E., Hahn, J., O'Dell, A.: Pertusate composition in organ preservation.Transplant. Proc., 3, 626 (1971).PubMedGoogle Scholar
  35. 35.
    Sacks, S. A., Petritsch, P. H., Kaufman, J. J.: Canine kidney preservation using a new perfusate.Lancet, 1, 1024 (1973).PubMedGoogle Scholar
  36. 36.
    Sharzer, L. A., Lawton, R. I.: Short-term organ preservation comparing two solutions and reimplantation with Nakayama staples.Ann. Surg., 179, 228 (1972).Google Scholar
  37. 37.
    Sterling, W. A., Datnow, B., Diethelm, A. G.: Sacks I solution and 7% mannitol solution for renal preservation.J. Surg. Res., 20, 103 (1976).PubMedGoogle Scholar
  38. 38.
    Stevens, L. E., Iverson, B. M., Reemtsma, K.: The preservation of kidneys byin vitro perfusion.Arch. Surg., 96, 540 (1968).PubMedGoogle Scholar
  39. 39.
    Szmolenszky, T., Garadnay, B., Szőke, P., Török, B.: Hydrogen polarography for the measurement of blood flow of canine myocardium with intact and affected circulation.Kisérl. Orvostud., 24, 179 (1972).Google Scholar
  40. 40.
    Török, B., Rőth, E. Szmolenszky, T., Tóth, I., Halmágyi, Gy., Lantos, J.: Description and interpretation of phenomena observed in donor kidneys. I. Morphology of adequate preservation.Acta chir. Acad. Sci. hung. (in press).Google Scholar
  41. 41.
    Török, B., Rőth, E., Szmolenszky, T.: Description and interpretation of phenomena observed in donor kidneys. II. Structural and functional alterations in the acute revascularization phase.Acta chir. Acad. Sci. hung. (in press).Google Scholar
  42. 42.
    Török, B., Tóth, I., Halmágyi, Gy., Rőth, E.: Description and interpretation of phenomena observed in donor kidneys. III. Angioarchitecture and function of reimplanted autografts.Acta chir. Acad. Sci. hung. (in press).Google Scholar
  43. 43.
    Woods, J. E., Holley, K. E., Pheteplace, E.: Kidney preservation by initial perfusion and surface cooling.J. Surg. Res., 11, 68 (1971).PubMedGoogle Scholar
  44. 44.
    Wu, M. S., Linke, C. A., Panner, B., Roberts, A., Cockett, T. K.: Early evaluation of renal function after prolonged hypothermic preservation.Invest. Urol., 11, 154 (1973).PubMedGoogle Scholar

Copyright information

© Akadämiai Kiadó 1978

Authors and Affiliations

  • E. Rőth
    • 1
  • T. Szmolenszky
    • 1
  • B. Török
    • 1
  1. 1.Department for Experimental SurgeryUniversity Medical SchoolPécsHungary

Personalised recommendations