Advertisement

Protoplasma

, Volume 148, Issue 2–3, pp 164–166 | Cite as

Mugineic acid secretion by cultured barley cells derived from anther

  • N. K. Nishizawa
  • S. Mori
  • S. Takahashi
  • T. Ueda
Rapid Communication

Summary

We succeeded in obtaining barley cell cultures which produce and secrete mugineic acid under the iron-deficient conditions. Cells derived from anther tissues of a six-rowed barley (Hordeum vulgare L. cv. Dissa) were cultured in the iron-deficient medium for 9 months and culture solutions were analyzed with HPLC and TLC at every transfer time. Cells cultured in the iron-deficient medium secreted at least five compounds which can solubilize iron, and one of them was identified as mugineic acid.

While the amounts of mugineic acid and other unknown compounds varied at each sampling time, changes in their amounts occurred in parallel periodically.

Keywords

Barley Iron-deficiency Mugineic acid Suspension culture 

Abbreviations

MAs

Mugineic acid and its derivatives: mugineic acid 2′-deoxymugineic acid, 3-hydroxymugineic acid, Avenic acid A, distichonic acid A

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chaney RL (1988) Recent progress and needed research in plant Fe nutrition. J Plant Nutr 11 (in press)Google Scholar
  2. Fushiya S, Sato Y, Nozoe S, Nomoto K, Takemoto T, Takagi S (1980) Tetrahedron Lett 21: 3071–3072Google Scholar
  3. Kawai S, Takagi S, Sato Y (1988) Mugineic acid-family phytosiderophores in root-secretions of barley, corn and sorghum varieties. J Plant Nutr 11: 633–642Google Scholar
  4. Luckey M, Pollack JR, Wayne R, Ames BN, Neilands JB (1972) Iron uptake inSalmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J Bacteriol 111: 731–738PubMedGoogle Scholar
  5. Marschner H, Romheld V, Kissel M (1986) Different strategies in higher plants in mobilization and uptake of iron. J Plant Nutr 9: 695–713Google Scholar
  6. Mino Y, Ishida T, Ota N, Inoue M, Nomoto K, Takemoto T, Tanaka H, Sugiura Y (1983) Mugineic acid-iron (III) complex: characterization and implication for absorption and transport of iron in gramineous plants. J Am Chem Soc 105: 4671–4676Google Scholar
  7. Mori S, Nishizawa N, Kawai S, Sato Y, Takagi S (1987) Dynamic state of mugineic acid and analogous phytosiderophores in Fe-deficient barley. J Plant Nutr 10: 1003–1011Google Scholar
  8. — —, (1987) Methionine as a dominant precursor of phytosiderophores inGraminaceae plants. Plant Cell Physiol 28: 1081–1092Google Scholar
  9. Nomoto K, Yoshioka H, Arima M, Fushiya S, Takagi S, Takemoto T (1981) Structure of 2′-deoxymugineic acid, a novel amino acid possessing an iron-chelating activity. Chimia 7: 249–250Google Scholar
  10. Takagi S (1976) Naturally occurring iron-chelating compounds in oat- and rice-root washings. Soil Sci Plant Nutr 22: 423–433Google Scholar
  11. —, Nomoto K, Takemoto T (1984) Physiological aspect of mugineic acid, a possible phytosiderophore of graminaceous plants. J Plant Nutr 7: 469–477Google Scholar
  12. Takahashi S, Kaneko T (1988) Isolation and culture of barley protoplast. Barley Genetics (in press)Google Scholar
  13. Takemoto T, Nomoto K, Fushiya S, Ouchi R, Kusano G, Hikino H, Takagi S, Matsuura Y, Kakudo M (1978) Structure of mugineic acid, a new amino acid possessing an iron-chelating activity from roots washing of water-culturedHordeum vulgare. Proc Japan Acad 54: 469–473Google Scholar
  14. Sugiura Y, Tanaka H, Mino Y, Ishida T, Ota N, Inoue M, Nomoto K, Yoshioka H, Takemoto T (1981) Structure, properties and transport mechanism of iron (III) complex of mugineic acid, a possible phytosiderophore. J Am Chem Soc 103: 6979–6982Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • N. K. Nishizawa
    • 1
  • S. Mori
    • 1
  • S. Takahashi
    • 2
  • T. Ueda
    • 1
  1. 1.Department of Agricultural Chemistry, Faculty of AgricultureThe University of TokyoTokyo
  2. 2.Plant Bioengineering Research LaboratorySapporo Brewery Ltd.Japan

Personalised recommendations