, Volume 148, Issue 2–3, pp 164–166 | Cite as

Mugineic acid secretion by cultured barley cells derived from anther

  • N. K. Nishizawa
  • S. Mori
  • S. Takahashi
  • T. Ueda
Rapid Communication


We succeeded in obtaining barley cell cultures which produce and secrete mugineic acid under the iron-deficient conditions. Cells derived from anther tissues of a six-rowed barley (Hordeum vulgare L. cv. Dissa) were cultured in the iron-deficient medium for 9 months and culture solutions were analyzed with HPLC and TLC at every transfer time. Cells cultured in the iron-deficient medium secreted at least five compounds which can solubilize iron, and one of them was identified as mugineic acid.

While the amounts of mugineic acid and other unknown compounds varied at each sampling time, changes in their amounts occurred in parallel periodically.


Barley Iron-deficiency Mugineic acid Suspension culture 



Mugineic acid and its derivatives: mugineic acid 2′-deoxymugineic acid, 3-hydroxymugineic acid, Avenic acid A, distichonic acid A


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • N. K. Nishizawa
    • 1
  • S. Mori
    • 1
  • S. Takahashi
    • 2
  • T. Ueda
    • 1
  1. 1.Department of Agricultural Chemistry, Faculty of AgricultureThe University of TokyoTokyo
  2. 2.Plant Bioengineering Research LaboratorySapporo Brewery Ltd.Japan

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