Acta Neurochirurgica

, Volume 87, Issue 1–2, pp 76–78 | Cite as

The study of the effect of human chorionic gonadotrophic (HCG) hormone on the survival of adrenal medulla transplant in brain. Preliminary study

  • A. Patil
  • K. Fillmore
  • J. Valentine
  • D. Hill


Autologous adrenal cortex was completely dissected off the medula using microdissection. The adrenal medulla tissue was transplanted to the lateral ventricle in twenty-one adult rats. Group A consisting of ten animals received 200 units of HCG per 100 gram body weight daily for one week and then on every alternate day for seven weeks. The remaining rats (group B) served as control. After eight weeks the brains were removed and a volumetric study was done using histological sections. High pressure liquid chromatography (HPLC) with electrochemical detection for catecholamine assay of the graft was also undertaken. The graft survival in group A was significantly higher (p<0.005) than group B. The level of norepinephrine was significantly higher in group A while dopamine was higher in group B. The higher volume of graft tissue in group A suggests that HCG may help in increased survival and growth of the transplanted tissue. Higher levels of norepinephrine in group A would suggest a tendency for such grafts to be active to a greater extent with the treatment of HCG.


Adrenal medulla transplant CNS transplantation catecholamine human chorionic gonadotrophic hormone 


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  1. 1.
    Backlund EO, Granberg PO, Hamberger B, Knutson E, Martensson A, Sedvall G, Seiger A, Olson L (1985) Transplantation of adrenal medullary tissue to striatum in Parkinsonism. J Neurosurg 62: 169–173PubMedGoogle Scholar
  2. 2.
    Bjorklund A, Dunnett SB, Stenevi Uet al (1980) Reinnervation of the devervated striatum by substantia nigra transplants: Functional consequences as revealed by pharmacological and sensorimotor testing. Brain Res 199: 307–333PubMedGoogle Scholar
  3. 3.
    Bjorklund A, Stenevi U (1977) Reformation of the severed septohippocampal cholinergic pathway in the adult rat by transplanted septal neurons. Cell Tissue Res 185: 289–302PubMedGoogle Scholar
  4. 4.
    Dunnett SB, Bjorklunds A, Stenevi Uet al (1981) Grafts of embryonic substantia nigra reinnervating the ventrolateral striatum ameliorate sensorimotor impairments and akinesia in rats with 6-OHDA lesions of the nigrostriatal pathway. Brain Res 229: 209–217PubMedGoogle Scholar
  5. 5.
    Freed WJ, Morihis JM, Spoor E,et al (1981) Transplanted adrenal chromaffin cells in rat brain reduce lesion-induced rotational behavior. Nature 192: 351–352Google Scholar
  6. 6.
    Freed WJ, Perslow MJ, Karoum Fet al (1960) Restoration of dopaminergic function by grafting of fetal rat substantia nigra in the caudate nucleus: long term behavioral biochemical and histological studies. Ann Neurol 8: 510–519Google Scholar
  7. 7.
    Jaeger CB, Lund RD (1980) Transplantation of embryonic occipital cortex to the brain of newborn rats. Exp Brain Res 40: 265–272PubMedGoogle Scholar
  8. 8.
    Olson L, Seiger A, Freedman Ret al (1980) Chromaffine cells can innervate brain tissue: evidence from intraocular double grafts. Exp Neurol 70: 414–426PubMedGoogle Scholar
  9. 9.
    Patil AA, Nagaraj MP (1983) The effect of Human Chorionic Gonadotropin (HCG) on functional recovery of spinal cord sectioned rats. Acta Neurochir (Wien) 69: 205–218Google Scholar
  10. 10.
    Sunde N, Zimmer J (1981) Transplantation of central nervous tissue. Acta Neurol Scand 63: 323–335PubMedGoogle Scholar
  11. 11.
    Unsicker K, Krisch B, Otten Uet al (1978) Nerve growth factor-induced fiber outgrowth from isolated rat adrenal chromaffin cells: impairment by glucocorticoids. Proc Natl Acad Sci USA 75: 3498–3502PubMedGoogle Scholar
  12. 12.
    Unsicker K, Tschechne B, Tschechne D (1981) Differentiation and transdifferentiation of adrenal chromaffin cells of the guinea-pig. Transplants to the anterior chamber of the eye. Cell Tissue Res 215: 341–367PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • A. Patil
    • 1
  • K. Fillmore
    • 1
  • J. Valentine
    • 2
  • D. Hill
    • 2
  1. 1.Division of NeurosurgeryOral Roberts University, School of Medicine, City of Faith Medical and Research CenterTulsaUSA
  2. 2.Department of PharmacologyOral Roberts University, School of Medicine, City of Faith Medical and Research CenterTulsaUSA

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