Advertisement

Protoplasma

, Volume 84, Issue 1–2, pp 191–196 | Cite as

Induction of haploid tobacco plants from isolated pollen

  • J. Reinert
  • Y. P. S. Bajaj
  • E. Heberle
Brief Report

Summary

The isolated pollen ofNicotiana tabacum cv. “Badischer Burley” obtained from coldtreated anthers (4 °C for 72 hours) cultured for 4 days, have been induced to undergo androgenesis on a synthetic medium enriched with amino acids. The advantages and potential of the culture of isolated pollen for the induction of haploids and mutation work are discussed.

Keywords

Tobacco Plant Synthetic Medium ofNicotiana Tabacum Mutation Work Haploid Tobacco 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bajaj, Y. P. S., 1974: Isolation and culture studies on pollen tetrad and pollen mother-cell protoplasts. Plant Sci. Letters3, 93–99.Google Scholar
  2. - 1975:In vitro induction of haploid plants. In: Modification of plant cells (Evans, P. K., ed.). Acad. Press (under preparation).Google Scholar
  3. Devreux, M., F. Saccardo etA. Brunori, 1971: Plantes haploïdes et lignes isogéniques deNicotiana tabacum obtenues par cultures d'anthères et tigesin vitro. Caryologia24, 141–148.Google Scholar
  4. Gresshoff, P. M., andC. H. Doy, 1972: Development and differentiation of haploidLycopersicon esculentum (Tomato). Planta107, 161–170.Google Scholar
  5. Guha, S., andS. C. Maheshwari, 1964:In vitro production of embryos from anthers ofDatura. Nature204, 497.Google Scholar
  6. Guha-Mukherjee, S., 1973: Genotypic differences in thein vitro formation of embryoids from rice pollen. J. exp. Bot.24, 139–144.Google Scholar
  7. Narayanaswamy, S., andL. P. Chandy, 1971:In vitro induction of haploid, diploid, and triploid androgenic embryoids and plantlets inDatura metel L. Ann. Bot.35, 535–542.Google Scholar
  8. Nishi, T., andS. Mitsuoka, 1969: Plants from anther and ovary culture of rice plant. Jap. J. Genet.44, 341–346.Google Scholar
  9. Nitsch, C., 1974: La culture de pollen isolé sur milieu synthétique. C. R. Acad. Sci. (Paris) Ser. D.278, 1031–1034.Google Scholar
  10. Nitsch, J. P., andC. Nitsch, 1969: Haploid plants from pollen grains. Science163, 85–87.Google Scholar
  11. Reinert, J., 1958: Untersuchungen über die Morphogenese an Gewebekulturen. Ber. dtsch. bot. Ges.71, 15.Google Scholar
  12. —, 1973: Aspects of organization—organogenesis and embryogenesis. In: Plant tissue and cell culture, pp. 338–355 (H. E. Street, ed.). Oxford: Blackwell Sci. Publ.Google Scholar
  13. Wagner, G., undD. Hess, 1974: Haploide, diploide und triploide Pflanzen vonPetunia hybrida aus Pollenkörnern. Z. Pflanzenphys.73, 273–276.Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • J. Reinert
    • 1
  • Y. P. S. Bajaj
    • 1
  • E. Heberle
    • 1
  1. 1.Institut für Pflanzenphysiologie und ZellbiologieFreie Universität BerlinDeutschland

Personalised recommendations