Advertisement

Agents and Actions

, Volume 20, Issue 3–4, pp 226–228 | Cite as

Isolation and sensitivity of human mesenteric mast cells to immunological and nonimmunological histamine releasers

  • E. Brzezińska-Błaszczyk
  • M. Czuwaj
Mast Cells and Basophils

Abstract

Since recent studies have emphasized that mast cells from different tissues within a given species may exhibit marked differences in their functional properties, we have now examined the effect of some immunological and non-immunological histamine releasers on human mesenteric mast cells. The mesentery derived from the patients subjected to gall-bladder surgery was dispersed by collagenase (concentration of enzyme — 1 mg/ml, time of incubation — 90 min, 37°C). The mesenteric cell suspension contained about 2% mast cells as identified by staining with toluidine blue. We observed that the mesenteric mast cells released histamine when challenged with anti-human IgE, but marked individual variations were observed. These cells had a low sensitivity to challenge with Concanavalin A and compound 48/80 (histamine release about 6%), but responded to ionophore A23187 and polymyxin B (histamine release up to 24% and 22% respectively).

Keywords

Cell Suspension Mast Cell Histamine Functional Property Collagenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    K. E. Barrett and D. D. Metcalfe,Mast cell heterogeneity: evidence and implications. J. Clin. Immunol.4, 253–261 (1984).PubMedGoogle Scholar
  2. [2]
    F. L. Pearce,Mast cell heterogeneity. Trends pharmacol. Sci.4, 165–167 (1983).Google Scholar
  3. [3]
    F. L. Pearce and M. Ennis,Isolation and some properties of mast cells from the mesentery of the rat and guinea pig. Agents and Actions10, 124–131 (1980).PubMedGoogle Scholar
  4. [4]
    P. A. Shore, A. Burkhalter and V. H. Cohn,A method for the fluorometric assay of histamine in tissues. J. Pharmacol. exp. Ther.127, 182–186 (1959).PubMedGoogle Scholar
  5. [5]
    M. Ennis,Histamine release from human pulmonary mast cells. Agents and Actions12, 60–63 (1982).PubMedGoogle Scholar
  6. [6]
    K. B. P. Leung, K. C. Flint, J. Brostoff, B. N. Hudspith, N. McI. Johnson and F. L. Pearce,Some properties of mast cells obtained by human bronchoalveolar lavage. Agents and Actions18, 110–112 (1986).PubMedGoogle Scholar
  7. [7]
    W. Schmutzler, K. Delmich, D. Eichelberg, S. Gluck, T. Greven, H. Jurgensen, K. P. Riesener, G. Risse and P. Pult,The human adenoidal mast cells. Susceptibility to different secretagogues and secretion inhibitors. Int. Archs Allergy appl. Immunol.77 177–178 (1985).Google Scholar
  8. [8]
    M. Ennis and F. L. Pearce,Differential reactivity of isolated mast cells from the rat and guinea-pig. Eur. J. Pharmacol.66, 339–345 (1980).PubMedGoogle Scholar
  9. [9]
    P. T. Peachell and F. L. Pearce,Some studies on the release of histamine from mast cells treated with polymyxin. Agents and Actions14, 379–385 (1984).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • E. Brzezińska-Błaszczyk
    • 1
  • M. Czuwaj
    • 1
  1. 1.Department of Biogenic AminesPolish Academy of SciencesŁódźPoland

Personalised recommendations