Agents and Actions

, Volume 20, Issue 3–4, pp 226–228 | Cite as

Isolation and sensitivity of human mesenteric mast cells to immunological and nonimmunological histamine releasers

  • E. Brzezińska-Błaszczyk
  • M. Czuwaj
Mast Cells and Basophils

Abstract

Since recent studies have emphasized that mast cells from different tissues within a given species may exhibit marked differences in their functional properties, we have now examined the effect of some immunological and non-immunological histamine releasers on human mesenteric mast cells. The mesentery derived from the patients subjected to gall-bladder surgery was dispersed by collagenase (concentration of enzyme — 1 mg/ml, time of incubation — 90 min, 37°C). The mesenteric cell suspension contained about 2% mast cells as identified by staining with toluidine blue. We observed that the mesenteric mast cells released histamine when challenged with anti-human IgE, but marked individual variations were observed. These cells had a low sensitivity to challenge with Concanavalin A and compound 48/80 (histamine release about 6%), but responded to ionophore A23187 and polymyxin B (histamine release up to 24% and 22% respectively).

Keywords

Cell Suspension Mast Cell Histamine Functional Property Collagenase 

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Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • E. Brzezińska-Błaszczyk
    • 1
  • M. Czuwaj
    • 1
  1. 1.Department of Biogenic AminesPolish Academy of SciencesŁódźPoland

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