Agents and Actions

, Volume 20, Issue 3–4, pp 157–160 | Cite as

The role of intracellular Ca2+ in the degranulation of skinned mast cells

  • K. Tasaka
  • M. Mio
  • M. Okamoto
Histamine Release


The intracellular pH of rat peritoneal mast cells was slightly acidic and compound 48/80 induced a decrease in the cytoplasmic pH of these cells. By means of chemical skinning, it was revealed that perfusion with Ca2+ or inositol 1,4,5-trisphosphate (IP3) induced degranulation dose-dependently in mast cells at concentrations higher than 10 μM and 0.1 μM, respectively. Na+ was essential for the release of histamine from mast cells. An assay based on the binding of45Ca to mast cell fragments revealed that the intracellular Ca store of the mast cell is located in the endoplasmic reticulum. IP3 liberated Ca from the endoplasmic reticulum.


Endoplasmic Reticulum Mast Cell Histamine Inositol Peritoneal Mast Cell 
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Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • K. Tasaka
    • 1
  • M. Mio
    • 1
  • M. Okamoto
    • 1
  1. 1.Department of Pharmacology, Faculty of Pharmaceutical SciencesOkayama UniversityOkayamaJapan

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