Abstract
A simple method was developed for the preparation of an autoclavable, long-side-chain poly (β-hydroxyalkanoate) (LSC-PHA) colloidal suspension, which was used as a substrate for enzymatic degradation and to prepare agar overlay plates for the isolation of microorganisms producing extracellular LSC-PHA depolymerase. Six cultures producing extracellular LSC-PHA depolymerase were isolated from a composted hydrocarbon-contaminated soil. All were pseudomonads or related bacteria. All (with the possible exception ofXanthomonas maltophilia) could produce LSC PHA. Except forX. maltophilia none could hydrolyze poly (β-hydroxybutyrate). Screening of sevenPseudomonas strains known to accumulate LSC PHA showed that all were negative for extracellular LSC-PHA depolymerase production. It was concluded that extracellular LSC-PHA depolymerase producers are found mostly in the genusPseudomonas but that they are relatively uncommon.
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References
L. L. Wallen and W. K. Rohwedder (1974)Environ. Sci. Technol. 8 576–579.
R. H. Findlay and D. C. White (1983)Appl. Environ. Microbiol. 45 71–78.
R. H. Marchessault, H. Morikawa, and J.-F. Revol (1984)Macromolecules 17 1882–1884.
M. J. de Smet, G. Eggink, B. Witholt, J. Kingma, and H. Wijnberg (1983)J. Bacteriol. 154 870–878.
R. G. Lageveen, G. W. Huisman, H. Preusting, P. Ketelaar, G. Eggink, and B. Witholt (1988)Appl. Environ. Microbiol. 54 2924–2932.
H. Brandl, R. A. Gross, R. W. Lenz, and R. C. Fuller (1988)Appl. Environ. Microbiol. 54 1977–1982.
A. Timm and A. Steinbüchel (1990)Appl. Environ. Microbiol. 56 3360–3367.
G. W. Haywood, A. J. Anderson, D. F. Ewing, and E. A. Dawes, (1990)Appl. Environ. Microbiol. 56 3354–3359.
B. Müller and D. Jendrossek (1993)Appl. Microbiol. Biotechnol. 38 487–492.
M. W. Stinson and J. M. Merrick (1974)J. Bacteriol. 119 152–161.
T. Tanio, T. Fukui, Y. Shirakura, T. Saito, K. Tomita, T. Kaiho, and S. Masamune (1982)Eur. J. Biochem. 124 71–77.
D. Jendrossek, I. Knoke, R. B. Habibian, A. Steinbüchel, and H. G. Schlegel (1993)J. Environ. Deg. Polym. 1 53–63.
C. L. Brucato and S. S. Wong (1991)Arch. Biochem. Biophys. 173 497–502.
K. Nakayama, T. Saito, T. Fukui, Y. Shirakura, and K. Tomita (1985)Biochim. Biophys. Acta 827 63–72.
F. P. Delafield, M. Doudoroff, N. J. Palleroni, C. J. Lusty, and R. Contopoulos (1965)J. Bacteriol. 90 1455–1466.
A. Schirmer, D. Jendrossek, and H. G. Schlegel (1993)Appl. Environ. Microbiol. 59 1220–1227.
B. A. Ramsay, I. Saracovan, M.-E. Nedea, R. H. Marchessault, and J. A. Ramsay (1992) inProceedings of the 42nd Canadian Chemical Engineering Conference, Toronto, Ontario, pp. 403–404.
B. A. Ramsay, I. Saracovan, J. A. Ramsay, and R. H. Marchessault (1992)Appl. Environ. Microbiol. 57 625–629.
D. M. Horowitz, J. Clauss, B. K. Hunter, and J. K. M. Sanders (1993)Nature 363 23.
G. Braunegg, B. Sonnleitner, and R. M. Lafferty (1978)Eur. J. Appl. Microbiol. Biotechnol. 6 29–37.
C. A. Lauzier, C. J. Monasterios, I. Saracovan, R. H. Marchessault, and B. A. Ramsay (1993)Tappi J. 76 71–77.
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Ramsay, B.A., Saracovan, I., Ramsay, J.A. et al. A method for the isolation of microorganisms producing extracellular long-side-chain poly (β-hydroxyalkanoate) depolymerase. J Environ Polym Degr 2, 1–7 (1994). https://doi.org/10.1007/BF02073481
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DOI: https://doi.org/10.1007/BF02073481