Abstract
Purpose: Cytogenetic studies of arrested oocytes and zygotes were used to understand in vitro fertilization (IVF) failures.
Methods: We investigated the cytogenetics (Giemsa banding and FISH) of 710 uncleaved oocytes and 94 arrested zygotes from 208 patients undergoing IVF procedures.
Results and Conclusions: Of uncleaved oocytes without a polar body, 39% were judged cytogenetically abnormal (17% unbalanced predivision and 21.5% diploid). Of 575 oocytes with a polar body, 124 (21.5%) showed numerical or structural chromosome aberrations. In arrested zygotes, approximately equal cases were found with separate condensed haploid complements (no syngamy), nuclear asynchrony and pulverized DNA, and apparently cytogenetically normal zygotes arrested at mitosis. These data on chromosome abnormalities were also analyzed with respect to two ovarian stimulation protocols and to maternal age. Both ovarian stimulation protocols showed the same levels of chromosome abnormalities. Overall chromosome abnormalities and premature chromosome condensation were also unchanged with maternal age. These data illustrate the significance of chromosome aberrations in IVF failures.
Similar content being viewed by others
References
Dalpra L, Tibiletti MG, Cristiani C, Ragni G, Grosignani PG: Morphology and nuclear contens in IVF embryos. Ann Genet 1993;36(2):100–106
Pellestor F, Girardet A, Andreo B, Arnal F, Humeau C: Relationship between morphology and chromosomal constitution in huma preimplantation embryos. Mol Reprod Dev 1994;39:141–146
Munne S, Dailey T, Sultan KM, Grifo J, Cohen J: The use of first polar bodies for preimplantation diagnosis of aneuploidy. Mol Hum Reprod 1995;10(4):1014–1020
Jacobs PA: The role of chromosome abnormalities in reproductive failure. Reprod Nutr Dev 1990:S1:63–74
Neveu S, Hedon B, Brinjer J, Chincholle JM, Arnal F, Humeau C, Cristol P, Viala JL: Ovarian stimulation by a combination of gonadotropin releasing hormone agonist and gonadotropins for in vitro fertilization. Fertil Steril 1987;47:639–643
Pouly JL, Janny L, Canis M, Vye P, Boyer C: Gamete intrafallopian transfer: Benefits of programmed stimulation. Fertil Steril 1989;52:1012–1017
Tarkowski AK: An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 1966:5:394
Tarin JJ, Gomez E, Pellicier A: Chromosomes anomalies in human oocytes in vitro. Fertil Steril 1991;55:964–969
Benkhalifa M, Malet P, Quimsiyeh M, Boucher D, Bellec V, Menezo Y: Chromosome aberrations in normal and translocated human sperm: Role in reproduction pathology. Ref Gynecol Obstet 1994;2(3):288–296.
Menezo Y, Benkhalifa M: Cytogenetic and cryobiology of human cocultured embryos: A 3-Year Experience. J Assist Reprod Genet 1995;12(1):35–40
Almeida PA, Bolton VN: Immaturity and chromosomal abnormalities in oocytes that fail to develop pronuclei following isemination in vitro. Hum Reprod 1994;8(2):229–232
Boue J, Boue A, Lazar P: Retrospective and prospective epidemiological studies of 1500 karyotype spontaneous human abortions. Teratology 1975;12:11–26
Mattei JF, Mattei MG, Ayme S, Giraud F: Origin of the extra-chromosome of trisomy 21. Hum Genet 1979;46: 107–110
Antonorakis SE, Petersen MB, McInnis MG, Adelsberger PA, Schinzel AA, Binkert F, Pangalos C, Raoul E, Slaugenhaupt SA, Hafez M, Cohen MM, Roulson D, Schwartz S, Mikkelsen M, Tranebjaerg L, Greenberg F, Hoard DI, Rudd NI, Warren AC, Mataxotou C, Bartsocas C, Chakravarti A: The meiotic stage of nondisjunction in trisomy 21: Determination by using DNA polymorphism. Am J Hum Genet 1992:50:544–550
Hawley RS, Frazier JA, Rasooly R: Commentary: Separation anxiety: The etiology of non-disjunction in flies and people. Hum Mol Genet 1994;3(9):1521–1528
Plachot M, Veiga A, Montagut J, de Grouchy J, Calderon G, Lepretre S, Junca AM, Santalos J, Carles E, Mandelbaum J, Barri P, Degoy J, Cohen J, Egozycue J, Sabatier JC, Salat Barroux J: Are clinical and biological IVF parameters correlated with chromosomal disorder in early life; A multicentric study. Hum Reprod 1988;3:627–635
Pellestor F, Sele B: Assessment of aneuploidy in human female by using cytogenetics of IVF failure. Am J Hum Genet 1988;42:274–283
Djalali M, Rosenbysc H, Wolf M, Sterzik K: Cytogenetic of infertilized human oocytes. J Reprod Fertil 1988;84:647–652
Eibling L, Colet M: Abnormal development and transport and increased sister-chromatide exchange in preimplantation embryos following superovulation in mice. Mutat Res 1985;147:185–189
Gras L, McBain J, Trounson A, Kola I: The incidence of chromosomal aneuploidy in stimulated and unstimulated (natural) unstimulated human oocytes. Hum Reprod 1992; 7(10):1396–1401
Pieters MHEC, Dumoulin JCM, Engelhart CM, Bras M, Evers JLH, Geraedts JPM: Immaturity and aneuploidy in human oocytes after different stimulation protocols. Fertil Steril 1991;56(2):306–311
Tejada MI, Mendoza MR, Corcostegul B, Benito JA: Factors associated with premature chromosome condensation (PCC) following in vitro fertilization. J Assist Reprod Genet 1992;9(1):61–66
Edirisinghe WR, Murch AR, Yovich JL: Cytogenetic analysis of human oocytes and embryos in an in vitro fertilization programme. Hum Reprod 1992;2:230–236
Wramsby M, Fredga K, Lieldhom: Chromosome analysis of human oocytes recovered from preovulatory in stimulated cycles. N Engl J Pediatr 1987;316:121–124
Martin RH, Mahadevan MM, Taylor PJ, Hildebrend K, Long Simpson L, Peterson D, Yamamoto J, Fleetham J: Chromosomal analysis of infertilized human oocytes. J Reprod Fertil 1986;78:673–678
Papadopoulos G, Templeton AA, Fish N, Randall J: The frequency of chromosome anomalies in human pre-implantation embryos after in vitro fertilization. Hum Reprod 1989; 4(1):91–98
Bongso A, Ng SC, Lim J, Fong SY, Ratnam S: Preimplantation genetics: Chromosomes of fragmented human embryos. Fertil Steril 1991;56(1):66–70
Schmialdy H, Sperling K, Kentenich M, Stauber M: Prematurely condensed chromosomes after in vitro fertilization (IVF). Hum Genet 1986;74:441–443
Janny L, Benkhalifa M, Pouly JL, Vye P, Canis M, Geneix A, Malet P, Boucher D: La condensation prematuree des chromosomes: Facteurs frequents d'echec en fecondation in vitro. Contracept Fertil Sex 1990;18:7–8
Benkhalifa M, Janny L, Vye P, Malet P, Boucher D, Bellec V, Menezo Y: Assessment of polyploidy in human morulae and blastocyst using co-culture and fluorescent in situ hybridization. Hum Reprod 1993:8(6):895–902
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Benkhalifa, M., Menezo, Y., Janny, L. et al. Cytogenetics of uncleaved oocytes and arrested zygotes in IVF programs. J Assist Reprod Genet 13, 140–148 (1996). https://doi.org/10.1007/BF02072536
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02072536