On the toxicity of low doses of tetrasodium-ethylenediamine-tetraacetate (Na-EDTA) in normal rat kidney (NRK) cells in culture
- 233 Downloads
The toxicity of tetrasodium-ethylenediaminetetraacetate (Na-EDTA) was investigated in normal rat kidney cells (NRK-52E, American Type Culture Collection) in culture. Cell death and reduced colony-forming ability were observed at Na-EDTA concentrations <100 μM, that is at concentrations lower than that required to chelate all the Ca2+ in the growth medium. No toxicity was observed when cells were exposed to 100 μM Zn- or Fe-EDTA, but the same concentration of Cu-EDTA was as toxic as Na-EDTA. Continuous exposure of the cell cultures to 5 μM Na- or Zn-EDTA for up to 7 weeks yielded no indications of toxicity.
Key wordsEDTA Cytotoxicity Kidney cell cultures
Unable to display preview. Download preview PDF.
- Brauch H, Schullerer S (1987) Verhalten von Ethylendiaminetetraacetat (EDTA) und Nitriloacetat (NTA) bei der Trinkwasseraufbereitung. Vom Wasser 69: 155–164Google Scholar
- FAO/WHO Food and Agriculture Organisation, World Health Organisation (1973) List of food additives evaluated for their safety for use in food. In Codex Alimentarius Commission/FAL.Google Scholar
- Hugenschmidt S (1990) Morphologische und biochemische Studien zur Toxizität von EDTA-Chelaten bei Rattennierenzellen in Kultur. Diplom Biologie Dissertation, University of Karlsruhe, GermanyGoogle Scholar