Archives of Toxicology

, Volume 67, Issue 1, pp 76–78 | Cite as

On the toxicity of low doses of tetrasodium-ethylenediamine-tetraacetate (Na-EDTA) in normal rat kidney (NRK) cells in culture

  • Stephanie Hugenschmidt
  • Felicitas Planas-Bohne
  • David M. Taylor
Short Communication

Abstract

The toxicity of tetrasodium-ethylenediaminetetraacetate (Na-EDTA) was investigated in normal rat kidney cells (NRK-52E, American Type Culture Collection) in culture. Cell death and reduced colony-forming ability were observed at Na-EDTA concentrations <100 μM, that is at concentrations lower than that required to chelate all the Ca2+ in the growth medium. No toxicity was observed when cells were exposed to 100 μM Zn- or Fe-EDTA, but the same concentration of Cu-EDTA was as toxic as Na-EDTA. Continuous exposure of the cell cultures to 5 μM Na- or Zn-EDTA for up to 7 weeks yielded no indications of toxicity.

Key words

EDTA Cytotoxicity Kidney cell cultures 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Stephanie Hugenschmidt
    • 1
  • Felicitas Planas-Bohne
    • 1
  • David M. Taylor
    • 1
  1. 1.Institut für Genetik und ToxikologieKernforschungszentrum KarlsruheKarlsruhe 1Germany

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