Abstract
In syntheses of naturally occurring amino acids labelled with short-lived radionuclides, such as11C, the determination of the enantiomeric purity presents problems. The aminoacyl coupling of L-amino acids to tRNA followed by a separation with gel filtration of the tRNA-amino acid complex and free amino acid, was here shown to be an adequate method for determining the enantiomeric purity of [methyl-11C]-methionine even in the pmol range. This method has also been used for the determination of the specific radioactivity of the11C-labelled methionine. The method is probably valid for other naturally occurring amino acids and it might also be of interest for enantiomeric separations of L- and D-amino acids of high specific radioactivity.
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Lundqvist, H., Långström, B. & Malmqvist, M. Determination of enantiomeric purity in biogenic11C-labelled amino acids by aminoacylation of tRNA. Journal of Radioanalytical and Nuclear Chemistry, Articles 89, 79–87 (1985). https://doi.org/10.1007/BF02070206
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DOI: https://doi.org/10.1007/BF02070206