Optical and Quantum Electronics

, Volume 18, Issue 1, pp 81–84 | Cite as

Highly sensitive fluorescence microscope system for single biological cell using laser excitation and digital image processing and display

  • Shun-ichi Sato
  • Hiromasa Ito
  • Humio Inaba
  • Yoshio Taguchi
  • Morio Kasai
Article
  • 27 Downloads

Abstract

A novel type of laser-excited fluorescence microscope system is reported, which, for the first time, realizes excellent sensitivity and spatial resolution in biological and medical applications using an ultra-high sensitivity image camera and digital image processing technique for three-dimensional display of the detected fluorescence intensity across a single cell. Experimental studies are also presented on the fluorescence intensity distribution of Rhodamine 6G adsorbed in an OH gel as a simple cell model and also of a hematoporphyrin-derivative (HpD) taken up by a cultured esophagus cancer cell.

Keywords

Fluorescence Intensity Rhodamine Image Camera Digital Image Processing Image Processing Technique 

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Copyright information

© Chapman and Hall Ltd. 1986

Authors and Affiliations

  • Shun-ichi Sato
    • 1
  • Hiromasa Ito
    • 1
  • Humio Inaba
    • 1
  • Yoshio Taguchi
    • 2
  • Morio Kasai
    • 2
  1. 1.Research Institute of Electrical CommunicationTohoku UniversitySendaiJapan
  2. 2.The 2nd Department of SurgeryTohoku University School of MedicineSendaiJapan

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