Journal of environmental polymer degradation

, Volume 3, Issue 4, pp 187–197 | Cite as

Confirmation of colonization of degrading bacterium strain SC-17 on poly(3-hydroxybutyrate) cast film

  • Haruo Nishida
  • Yutaka Tokiwa


To clarify the mechanism of microbial degradation owing to colonization ofPseudomonas sp. strain SC-17 on a poly(3-hydroxybutyrate) (PHB) cast film surface, morphological and spectroscopic analyses of the degraded film were investigated and colonization kinetics on the films is discussed. By spectroscopic analysis of unique hemispherical degradation marks, cells of strain SC-17 adhering to the marks' surface were confirmed. To account for the hemispherical hole formation and their linear enlargement with culture time, a three-dimensional colony growth model toward the interior of the film was developed. The model explained the hemispherical hole formation well. It was concluded that the hemispherical holes resulted from the colonization of strain SC-17 on the film surfaces. It was further determined that the microbial degradation by strain SC-17 is initiated from small pits formed on the PHB film surface.

Key words

Poly(3-hydroxybutyrate) film biodegradation microbial degradation colonization colonization kinetics 


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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Haruo Nishida
    • 1
  • Yutaka Tokiwa
    • 2
  1. 1.Tsukuba Research LaboratoryTokuyama CorporationTsukuba, IbarakiJapan
  2. 2.National Institute of Bioscience and Human-TechnologyTsukuba, IbarakiJapan

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