Advertisement

Journal of environmental polymer degradation

, Volume 3, Issue 4, pp 187–197 | Cite as

Confirmation of colonization of degrading bacterium strain SC-17 on poly(3-hydroxybutyrate) cast film

  • Haruo Nishida
  • Yutaka Tokiwa
Article

Abstract

To clarify the mechanism of microbial degradation owing to colonization ofPseudomonas sp. strain SC-17 on a poly(3-hydroxybutyrate) (PHB) cast film surface, morphological and spectroscopic analyses of the degraded film were investigated and colonization kinetics on the films is discussed. By spectroscopic analysis of unique hemispherical degradation marks, cells of strain SC-17 adhering to the marks' surface were confirmed. To account for the hemispherical hole formation and their linear enlargement with culture time, a three-dimensional colony growth model toward the interior of the film was developed. The model explained the hemispherical hole formation well. It was concluded that the hemispherical holes resulted from the colonization of strain SC-17 on the film surfaces. It was further determined that the microbial degradation by strain SC-17 is initiated from small pits formed on the PHB film surface.

Key words

Poly(3-hydroxybutyrate) film biodegradation microbial degradation colonization colonization kinetics 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    H. Brandl, R. A. Gross, R. W. Lenz, and R. C. Fuller (1990)Adv. Biochem. Eng./Biotechnol. 41, 77–93.Google Scholar
  2. 2.
    P. H. Janssen and C. G. Harfoot (1990)Arch. Microbiol. 154, 253–259.Google Scholar
  3. 3.
    D. Jendrossek, I. Knoke, R. B. Habibian, A. Steinbüchel, and H. G. Schlegel (1993)J. Environ. Polym. Degrad. 1, 53–63.Google Scholar
  4. 4.
    J. Mergaert, A. Webb, C. Anderson, A. Wouters, and J. Swings (1993)Appl. Environ. Microbiol. 59, 3233–3238.PubMedGoogle Scholar
  5. 5.
    H. Nishida and Y. Tokiwa (1993)J. Environ. Polym. Degrad. 1, 227–233.Google Scholar
  6. 6.
    H. Nishida and Y. Tokiwa (1993)Kobunshi Ronbunshu 50, 739–746.Google Scholar
  7. 7.
    Y. Doi, Y. Kanesawa, M. Kunioka, and T. Saito (1990)Macromolecules 23, 26–31.Google Scholar
  8. 8.
    Y. Kumagai, Y. Kanesawa, and Y. Doi (1992)Makromol. Chem. 193, 53–57.Google Scholar
  9. 9.
    K. Mukai, K. Yamada, and Y. Doi (1992)Int. J. Biol. Macromol. 14, 235–239.PubMedGoogle Scholar
  10. 10.
    J. J. Jesudason, R. H. Marchessault, and T. Saito (1993)J. Environ. Polym. Degrad. 1, 89–98.Google Scholar
  11. 11.
    H. Nishida and Y. Tokiwa (1993)J. Environ. Polym. Degrad. 1, 65–80.Google Scholar
  12. 12.
    R. W. Lenz (1993)Adv. Polym. Sci. 107, 1–40.Google Scholar
  13. 13.
    H. Nishida and Y. Tokiwa (1992)J. Appl. Polym. Sci. 46, 1467–1476.Google Scholar
  14. 14.
    W. J. Cook, J. A. Cameron, J. P. Bell, and S. J. Huang (1981)J. Polym. Sci. Polym. Lett. Ed. 19, 159–165.Google Scholar
  15. 15.
    S. Akita, Y. Einaga, Y. Miyake, and H. Fujita (1976)Macromolecules 9, 774–780.Google Scholar
  16. 16.
    Y. Tsunoda, I. Aishima, and K. Katayama (1959)Kobunshi Kagaku 16, 491–494.Google Scholar
  17. 17.
    T. Hasegawa (1985)Biseibutu no bunrui to doutei, Gakkai Shuppan Center, Tokyo.Google Scholar
  18. 18.
    S. J. Pirt (1967)J. Gen. Microbiol. 47, 181–197.PubMedGoogle Scholar
  19. 19.
    S. A. Palumbo, M. G. Johnson, V. T. Rieck, and L. D. Witter (1971)J. Gen. Microbiol. 66, 137–143.PubMedGoogle Scholar
  20. 20.
    V. T. Rieck, S. A. Palumbo, and L. D. Witter (1973)J. Gen. Microbiol. 74, 1–8.PubMedGoogle Scholar
  21. 21.
    R. Hattori, T. Hattori, and C. Furukawa (1972)J. Gen. Appl. Microbiol. 18, 271–283.Google Scholar
  22. 22.
    R. Hattori, T. Hattori, and C. Furukawa (1972)J. Gen. Appl. Microbiol. 18, 285–293.Google Scholar
  23. 23.
    K. Saido, Y. Ishihara, M. Inoue, T. Kuroki, and Y. Kabasawa (1991)Mater. Life 3, 218–224.Google Scholar

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Haruo Nishida
    • 1
  • Yutaka Tokiwa
    • 2
  1. 1.Tsukuba Research LaboratoryTokuyama CorporationTsukuba, IbarakiJapan
  2. 2.National Institute of Bioscience and Human-TechnologyTsukuba, IbarakiJapan

Personalised recommendations