Purpose: The study was conducted to evaluate levels of anticomplement in seminal plasma and levels of complement in follicular fluid, in correlation with fertilization and pregnancy rate after in vitro fertilization and intracytoplasmic sperm injection programs.
Materials and Methods: Anticomplement levels were determined in 70 couples undergoing in vitro fertilization therapy. In 15 of these couples, complement levels were measured. Anticomplement and complement levels were also determined in an additional 21 couples (apart from the 70 couples) undergoing intracytoplasmic sperm injection treatment.
Results: A correlation was found between fertilization rate and anticomplement levels in the seminal plasma (r=0.4,P<0.01) after standard in vitro fertilization. No correlation was found in the intracytoplasmic sperm injection group, or observed between complement levels and any parameter examined in both groups. Pregnancy occurred only in those couples with an anticomplement: complement ratio below 0.49.
Conclusions: Determination of anticomplement and complement levels may contribute to the assessment of a successful outcome of in vitro fertilization/intracytoplasmic sperm injection.
anticomplement complement in vitro fertilization intracytoplasmic sperm injection
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Vanderpuye OA, Labarrere CA, McIntyre JA: The complement system in human reproduction. Am J Reprod Immunol 1992;27:145–155PubMedGoogle Scholar
Rooney IA, Oglesby TJ, Atkinson JP: Complement in human reproduction: activation and control. Immunol Res 1993;12:276–294PubMedGoogle Scholar
Anderson DJ, Abbott AF, Jack RM: The role of complement component C3b and its receptors in sperm-oocyte interaction. Proc Natl Acad Sci USA 1993;90:10051–10055PubMedGoogle Scholar
Hasty LA, Brockman WW, Lambris JD, Lyttle CR: Hormonal regulation of complement factor B in human endometrium. Am J Reprod Immunol 1993;30:63–67PubMedGoogle Scholar
Tesarik J: Comparison of acrosome reaction-inducing activities of human cumulus oophorus, follicular fluid and ionophore A23187 in human sperm populations of proven fertilization ability in vitro. J Reprod Fertil 1985;74:383–388PubMedGoogle Scholar
Fakih H, Vijayakumai R: Improved pregnancy rates and outcome with gamete intrafallopian transfer when follicular fluid is used as a sperm capacitation and gamete transfer medium. Fertil Steril 1990;53:515–527PubMedGoogle Scholar
Barak Y, Lessing JB, Amit A, Kogosowski A, Yovel I, David MP, Peyser MR: The development of an efficient ambulatory in vitro fertilization and embryo transfer program (ET) using ultrasonically-guided oocyte retrieval. Acta Obstet Gynecol Scand 1988;67:585–588PubMedGoogle Scholar
Nagy Z, Liu J, Cecile J, Silber S, Devroey P, van Steirtegheim A: Using ejaculated, fresh and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil Steril 1995;63:808–819PubMedGoogle Scholar
World Health Organization Laboratory Manual for Examination of Human Semen. Cambridge, Cambridge University Press, 1987Google Scholar
Price RJ, Roberts TK, Green D, Boetteher B: Anticomplementary activity in human semen and its possible importance in reproduction. Am J Reprod Immunol 1984;6:92–93PubMedGoogle Scholar