Abstract
Purpose: The efficacy of homologous oviductal cell coculture on the success of a humanin vitro fertilization program was investigated in a prospective randomized control clinical trial.
Methods and Results: One hundred eighty-one couples were randomized into the control and the coculture groups. Pronu-clear-stage zygotes were either cultured in Earles' balanced salt solution supplemented with 15% preovulatory serum (control) or cultured with human oviductal cells (coculture) for 24 hr before embryo transfer. There was no difference in the age of the patients, indication for treatment, number of oocyte retrieved or fertilized, or number of embryo replaced between the two groups. The pregnancy rates per transfer for the control and the coculture group were 12.8 and 19.3%, respectively. The number of viable fetus was significantly higher (P<0.01, chi-square test) in the coculture group (25/264) than in the control group (8/262). The coculture group also showed a higher multiple pregnancy rate, lower abortion rate, and more spare embryos suitable for cryopreservation.
Similar content being viewed by others
References
Menezo YJR, Guerin JF, Czyba JC: Improvement of human early embryo developmentin vitro by coculture on monolayer of Vero cells. Biol Reprod 1990;42:301–306
Wiemer KE, Cohen J, Amborski GF, Munuyakani L, Wiker S, Godke RA:In-vitro development and implantation of human embryos following culture on fetal uterine fibroblast cells. Hum Reprod 1989;4:595–600
Wiemer KE, Cohen J, Wiker SR, Malter HE, Wright G, Godke RA: Coculture of human zygotes on fetal bovine uterine fibroblasts: Embryonic morphology and implantation. Fertil Steril 1989;52:503–508
Wiemer KE, Hoffman DI, Maxson WS, Eager S, Muhlberger B, Fiore I, Cuervo M: Embryonic morphology and rate of implantation of human embryos following coculture on bovine oviductal epithelial cells. Hum Reprod 1993;8:97–101
Freeman MR, Whitworth CM, Hill AH: Granulosa cell coculture enhances human embryo development and pregnancy rate followingin vitro fertilization. Hum Reprod 1995;10:408–414
Mansour RT, Aboulghar MA, Serour GI, Abbass AM: Coculture of human pronucleate oocytes with their cumulus cells. Hum Reprod 1994;9:1727–1729
Bongso A, Ng SC, Sathananthan H, Ng PL, Rauff M, Ratnam S: Improved quality of human embryos when co-cultured with human ampullary cells. Hum Reprod 1989;4:706–713
Bongso A, Ng SC, Fong CY, Anandakumar C, Marshall B, Edirisinghe R, Ratnam S: Improved pregnancy rate after transfer of embryos grown in human fallopian tubal cell coculture. Fertil Steril 1992;58:569–574
Yeung WSB, Ho PC, Lau EYL, Chan STH: Improved development of human embryosin vitro by a human oviductal cell coculture system. Hum Reprod 1992;7:1144–1149
Desai NN, Kennard EA, Kniss DA, Friedman CI: Novel human endometrial cell line promotes blastocyst development. Fertil Steril 1994;61:760–766
Bongso A, Fong CY, Ng SC, Ratnam S: Human embryonic behavior in a sequential human oviduct-endometrial coculture system. Fertil Steril 1994;61:976–978
Van Blerkom J: Development of human embryos to the hatched blastocyst stage in the presence or absence of a monolayer of Vero cells. Hum Reprod 1993;8:1525–1539
Sakkas D, Jaquenoud N, Leppens G, Campana A: Comparison of results afterin vitro fertilized human embryos are cultured in routine medium and in coculture on Vero cells: A randomized study. Fertil Steril 1994;61:521–525
Liu LPS, Yeung WSB, Chan STH: Effects of changes in concentration of pyruvate and lactate on embryo development during coculture of human oviductal cells with mouse embryos.In Abstracts for the Second Inter-Congress Symposium of the Asia and Oceania Society for Comparative Endocrinology, Chiang Mai, Thiland, Oct 1993
Liu LPS, Chan STH, Ho PC, Yeung WSB: Human oviductal cells produce high molecular weight factor(s) that improves the development of mouse embryo. Hum Reprod 1995;10:2781–2786
Meinert CL: Randomization and the mechanics of treatment masking. In Clinical Trials: Design, Conduct and Analysis, CL Meinert (ed). New York/Oxford, Oxford University Press, 1986, pp. 90–112
Schillaci R, Ciriminna R, Cefalu E: Vero cell effect onin-vitro human blastocyst development: preliminary results. Hum Reprod 1994;9:1131–1135
Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, Wiemer K: Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod 1990;5:7–13
Jayot S, Parneix I, Verdaguer S, Discamps G, Audebert A, Emperaire JC: Coculture of embryos on homologous endometrial cells in patients with repeated failures of implantation. Fertil Steril 1995;63:109–114
Nagao Y, Saeki K, Hoshi M, Kainuma H: Effects of oxygen concentration and oviductal epithelial tissue on the development ofin vitro matured and fertilized bovine oocytes cultured in protein-free medium. Theriogenology 1994;41:681–688
Watson AJ, Watson PH, Warnes D, Walker SK, Armstrong DT, Seamark RF: Preimplantation development ofin vitro-matured andin vitro-fertilized ovine zygotes: Comparison between coculture on oviduct epithelial cell monolayer and culture under low oxygen atmosphere. Biol Reprod 1994;50:715–724
Batt PA, Gardner DK Cameron WN: Oxygen concentration and protein source affect the development of preimplantation goat embryosin vitro. Reprod Fertil Dev 1991;3:601–607
Johnston LA, Donoghue AM, O'Brien SJO, Wildt DE: Influence of temperature and gas atmosphere onin-vitro fertilization and embryo development in domestic cats. J Reprod Fertil 1991;92:377–382
Li J, Foote RH: Culture of rabbit zygotes into blastocysts in protein-free medium with one to twenty per cent oxygen. J Reprod Fertil 1993;98:163–167
Fabon JE Jr, Findley WE, Gibbons WE: The toxic effect of short exposures to the atmospheric oxygen concentration on early mouse embryonic development. Fertil Steril 1989;51:896–900
Umaoka Y, Noda Y, Narimoto K, Mori T: Effects of oxygen toxicity on early development of mouse embryos. Mol Reprod Dev 1992;31:28–33
Nasr-Esfahani M, Winston NJ, Johnson MH: Effects of glucose, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryoin vitro. J Reprod Fertil 1992;96:219–231
Ali J, Whitten WK, Shelton JN: Effect of culture systems on mouse early embryo development. Hum Reprod 1993;8:1110–1114
Feichtinger W, Kemeter P, Szalay S: The Vienna program ofin vitro fertilization and embryo-transfer—A successful clinical treatment. Eur J Obstet Gynecol Reprod Biol 1983;15:63–70
Dumoulin JCM, Vanvuchelen RCM, Land JA, Pieters MHEC, Geraedis JPM, Evers JLH: Effect of oxygen concentration onin vitro fertilization and embryo culture in the human and the mouse. Fertil Steril 1995;63:115–119
Noda Y, Narimoto K, Umaoka Y, Takakura K, Kanzaki H, Taii S, Mori T: Improved blastulation rate in human embryo culture with α-MEM under low oxygen. 1991;Suppl O-159 (abstract)
Bongso A, Ng SC, Fong CY, Ratnam S: Cocultures: A new lead in embryo quality improvement for assisted reproduction. Fertil Steril 1991;56:179–191
Olivennes F, Hazout A, Lelaidier C, Freitas S, Fanchin R, de Ziegler D, Frydman R: Four indications for embryo transfer at the blastocyst stage. Hum Reprod 1994;9:2367–2373
Menezo Y, Hazout A, Dumont M, Herbaut N, Nicollet B: Coculture of embryos on Vero cells and transfer of blastocysts in human. Hum Reprod 1992;7:101–106
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yeung, W.S.B., Lau, E.Y.L., Chan, S.T.H. et al. Coculture with homologous oviductal cells improved the implantation of human embryos—A prospective randomized control trial. J Assist Reprod Genet 13, 762–767 (1996). https://doi.org/10.1007/BF02066494
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02066494