Journal of Assisted Reproduction and Genetics

, Volume 13, Issue 9, pp 726–730 | Cite as

Could in vitro fertilization with a modified sperm preparation technique be an option to micromanipulations?

  • Christiane Wittemer
  • Jeanine Ohl
  • Karima Bettahar-Lebugle
  • Laurence Moreau
  • Pierre Dellenbach


Purpose: This study evaluated the efficiency of in vitro fertilization with a modified sperm preparation technique for male infertility, to avoid systematic recourse to ICSI.

Methods: The sperm function stimulants pentoxifylline, 2-deoxyadenosine, and follicular fluid were used during the sperm preparation of IVF trials for two groups of patients: couples with long-standing infertility and previous IVF failures and couples with male-factor infertility scheduled for their first treatment.

Results: Forty-nine attempts were performed in the first group. The overall fertilization rate (percentage of zygotes with two pronuclei 18 hr after sperm insemination) was 30% and the clinical pregnancy rate per transfer was 33%. For the second group, 30 attempts were performed. The fertilization rate was 29.6% and the clinical pregnancy rate was 62.5% per transfer. The total number of recovered motile sperm appears to be a significant parameter: a cutoff value of 0.25 million motile sperm recovered after capacitation treatment has a good prognostic value for choosing between IVF with sperm enhancers and ICSI.

Conclusions: The described modified IVF could constitute an alternative to the systematic use of ICSI.

Key words

follicular fluid intracytoplasmic sperm injection pentoxifylline 2-deoxyadenosine 


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  1. 1.
    Van Steirteghem A, Nagy Z, Joris H, Liu J, Staessen C, Smitz J, Wisanto A, Devroey P: High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993;8:1061–1066PubMedGoogle Scholar
  2. 2.
    Yovich J, Edirisinghe W, Cummins J, Yovich J: Influence of pentoxifylline in severe male factor infertility. Fertil Steril 1990;53:715–722PubMedGoogle Scholar
  3. 3.
    Yovich J: Pentoxifylline: Actions and applications in assisted reproduction. Hum Reprod 1993;8:1786–1791PubMedGoogle Scholar
  4. 4.
    Imoedemhe D, Sigue A, Pacpaco E, Olazo A: In vitro fertilization and embryonic development of oocytes fertilized by sperm treated with 2-deoxyadenosine. Int J Fertil 1993;38:235–240Google Scholar
  5. 5.
    Tournaye H, Wieme P, Janssens R, Verheyen G, Devroey P, Van Steirteghem A: Incubation of spermatozoa from asthenozoospermic semen samples with pentoxifylline and 2-deoxyadenosine: variability in hyperactivation and acrosome reaction rates. Hum Reprod 1994;9:2038–2043PubMedGoogle Scholar
  6. 6.
    Yee B, Cummings L: Modification of the sperm penetration assay using human follicular fluid to minimize false negative results. Fertil Steril 1988;50:123–128PubMedGoogle Scholar
  7. 7.
    Morales P, Llanos M, Gutierrez G, Kohen P, Vigil P, Vantman D: The acrosome reaction-inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content. Hum Reprod 1992;7:646–651PubMedGoogle Scholar
  8. 8.
    David G, Bisson JP, Czyglik F, Jouannet P, Gernigon C: Anomalies morphologiques du spermatozoïde humain. J Gyn Obstet Biol Reprod 1975;4:17–36Google Scholar
  9. 9.
    Veeck L: Typical morphology of the human oocyte and conceptus.In Atlas of the Human Oocyte and Early Conceptus. Baltimore, Williams and Wilkins, 1991, Vol 2, pp 1–99Google Scholar
  10. 10.
    Kaskar K, Franken D, Van der Horst G, Kruger T: The effect of pentoxifylline on sperm movement characteristics and zona pellucida binding potential of teratozoospermic men. Hum Reprod 1994;9:477–481PubMedGoogle Scholar
  11. 11.
    Tournaye H, Janssens R, Verheyen G, Devroey P, Van Steirteghem A: In vitro fertilization in couples with previous fertilization failure using sperm incubated with pentoxifylline and 2-deoxyadenosine. Fertil Steril 1994;62:574–579PubMedGoogle Scholar
  12. 12.
    Mbizvo M, Johnston R, Baker G: The effect of the motility stimulants, caffeine, pentoxifylline and 2-deoxyadenosine on hyperactivation of cryopreserved human sperm. Fertil Steril 1993;59:1112–1117PubMedGoogle Scholar
  13. 13.
    Matson PL, Yovich J, Edirisinghe W, Junk S, Yovich J: An argument for the past and continued use of pentoxifylline in assisted reproductive technology. Hum Reprod 1995;10(1):67–71Google Scholar

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Christiane Wittemer
    • 1
  • Jeanine Ohl
    • 1
  • Karima Bettahar-Lebugle
    • 1
  • Laurence Moreau
    • 1
  • Pierre Dellenbach
    • 1
  1. 1.Departement de Procréation Médicalement AssistéeCentre Médico-Chirurgical et ObstétricalSchiltigheim CedexFrance

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