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Journal of Assisted Reproduction and Genetics

, Volume 13, Issue 9, pp 726–730 | Cite as

Could in vitro fertilization with a modified sperm preparation technique be an option to micromanipulations?

  • Christiane Wittemer
  • Jeanine Ohl
  • Karima Bettahar-Lebugle
  • Laurence Moreau
  • Pierre Dellenbach
Andrology

Abstract

Purpose: This study evaluated the efficiency of in vitro fertilization with a modified sperm preparation technique for male infertility, to avoid systematic recourse to ICSI.

Methods: The sperm function stimulants pentoxifylline, 2-deoxyadenosine, and follicular fluid were used during the sperm preparation of IVF trials for two groups of patients: couples with long-standing infertility and previous IVF failures and couples with male-factor infertility scheduled for their first treatment.

Results: Forty-nine attempts were performed in the first group. The overall fertilization rate (percentage of zygotes with two pronuclei 18 hr after sperm insemination) was 30% and the clinical pregnancy rate per transfer was 33%. For the second group, 30 attempts were performed. The fertilization rate was 29.6% and the clinical pregnancy rate was 62.5% per transfer. The total number of recovered motile sperm appears to be a significant parameter: a cutoff value of 0.25 million motile sperm recovered after capacitation treatment has a good prognostic value for choosing between IVF with sperm enhancers and ICSI.

Conclusions: The described modified IVF could constitute an alternative to the systematic use of ICSI.

Key words

follicular fluid intracytoplasmic sperm injection pentoxifylline 2-deoxyadenosine 

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Christiane Wittemer
    • 1
  • Jeanine Ohl
    • 1
  • Karima Bettahar-Lebugle
    • 1
  • Laurence Moreau
    • 1
  • Pierre Dellenbach
    • 1
  1. 1.Departement de Procréation Médicalement AssistéeCentre Médico-Chirurgical et ObstétricalSchiltigheim CedexFrance

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