Coculture of human spermatozoa with reproductive tract cell monolayers can enhance sperm functions better than coculture with vero cell monolayers

  • Y. M. Lai
  • F. H. Chang
  • C. L. Lee
  • J. D. Lee
  • H. Y. Huang
  • M. L. Wang
  • P. J. Chan
  • M. Y. Chang
  • Y. K. Soong
Andrology

Abstract

Purpose: In order to develop a better system for support of human sperm function in vitro, we conducted studies to evaluate whether reproductive tract cells are better than non-reproductive tract cells as an adjunt in that regard.

Methods: Human spermatozoa were cocultured with Vero cells, with human oviduct cells and endometrial cells, and without cells (control) for either 1, 4, or 24 hr. Sperm motility was then analyzed with a computer-aided sperm analyzer (CASA-Hamiliton Thron, HTM IVOS Motility Analyzer). Aliquots of spermatozoa incubated for 24 hr were also stained with Hoechst 33258 and FITC-PNA to evaluate the status of acrosome in live cells.

Results: Significant differences (P<0.05) between the oviduct cell and the control groups after 24 hr were evident in the curvilinear velocity (VCL) (81.4±13.4 vs 60.0±14.1 µm/sec) and amplitude of lateral head displacement (ALH) (5.2±0.6 vs 4.1±0.5 µm). The incidence of acrosome reaction of live sperm was significantly higher in the endometrial cell group than in the controls (25.4±9.9 vs 6.6±2.4%;P<0.001).

Conclusions: Coculture with human reproductive tract cells seems to improve some functional parameters of human spermatozoa. Coincubation with such cell lines, especially oviduct cells, might be a feasible approach to optimization of human spermatozoa for assisted fertilization using subfertile or frozen-thawed samples. We think coincubating human spermatozoa with a human reproductive tract cell line, especially oviduct cells, might be a feasible approach in preparing human spermatozoa for assisted fertilization in subfertile and frozen-thawed semen samples.

Key words

coculture acrosome reaction Vero cell human oviduct cell human endometrial cell 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Yanagimachi R: Mammalian fertilization.In Physiology of Reproduction, E Knobi, JD Neill (eds). New York, Raven Press, 1988, Vol 1, pp 135–185Google Scholar
  2. 2.
    Bongso A, Ng SC, Fong CY, Ratnamm SS: Cocultures: A new lead in embryos in embryo quality improvement for assisted reproduction. Fertil Steril 1991;56:179–191PubMedGoogle Scholar
  3. 3.
    Bongso A, Ho J, Fong CY, Ng SC, Ratnam S: Human sperm function after coculture with human fallopian tubal epithelial cell monolayers: In vitro model for studing cell interactions in early human conception. Arch Androl 1993;31:183–190PubMedGoogle Scholar
  4. 4.
    Lai YM, Stein DE, Soong YK, Tang YX, Grifo J, Malter HE: Evaluation of Vero cell coculture system for mouse embryos in various media. Hum Reprod 1992;7:276–280PubMedGoogle Scholar
  5. 5.
    Soong YK, Lai YM, Chang SY, Wang ML, Chang MY, Lee CL: A successful pregnancy after subzonal insertion of epididymal sperm and coculture on Vero cell monolayer. Fertil Steril 1993;59:1308–1310PubMedGoogle Scholar
  6. 6.
    Chen CS, Chu SH, Soong YK, Lai YM: Epididymal sperm aspiration with assisted reproductive techniques: Difference between congenital and acquired obstructive azoospermia? Hum Reprod 1995;10:1104–1108PubMedGoogle Scholar
  7. 7.
    Guelrin JF, Ouhibi N, Regnier-Vigouroux G, Menezo Y: Movement characteristics and hyperactivation of human sperm on different epithelial cell monolayers. Int J Androl 1991;14:412–422PubMedGoogle Scholar
  8. 8.
    Wetzels AMM, Bastiaana BA, Goverde HJM, Janssen HJ, Rolland R: Vero cells stimulate human sperm motility in vitro. Fertil Steril 1991;56:535–539PubMedGoogle Scholar
  9. 9.
    Pearlstone AC, Chan SYW, Tucker MJ, Wiker SR, Wang C: The effects of Vero (Green monkey kiney) cell coculture on the motility patterns of cryopreserved human spermatozoa. Fertil Steril 1993;59:1105–1111PubMedGoogle Scholar
  10. 10.
    Chen HF, Ho H-N, Chen SU, Lien YR, Chao KH, Lin HR, Huang SC, Lee TY, Yang YS: Co-culture with Vero cell monolayer maintains the motility of asthenozoospermic semen samples Hum Reprod 1994;9:1276–1280PubMedGoogle Scholar
  11. 11.
    Bongso A, Fong CY, Ng SC, Ratnam SS: Human embryonic behavior in a sequential human oviduct-endometrial coculture system. Fertil Steril 1994;60:976–978Google Scholar
  12. 12.
    Fusi FM, Vigano P, Daverio R, Busacca M, Vignali M: Effects of the coculture with human endometrial cells on the function of spermatozoa from subfertile men. Fertil Steril 1994;61:160–167PubMedGoogle Scholar
  13. 13.
    Kervancioglu ME, Diahanbahch O, Aitken RJ: Epithelial cell coculture and the induction of sperm capacitation. Fertil Steril 1994;61:1103–1108PubMedGoogle Scholar
  14. 14.
    Goldman EE, Ellington JE, Farrell PB, Foote EH: Use of fresh and frozen-thawed bovine oviduct cell for acrosomal reacting fresh and frozen thawed bull sperm in vitro. Theriogenology 1991;35:204–223Google Scholar
  15. 15.
    Ouhibi N, Hamidi J, Guillaud J, Menezo Y: Co-culture of 1-cell mouse embryos on different cell supports. Hum Reprod 1990;5:734–737Google Scholar

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Y. M. Lai
    • 1
  • F. H. Chang
    • 1
  • C. L. Lee
    • 1
  • J. D. Lee
    • 1
  • H. Y. Huang
    • 1
  • M. L. Wang
    • 1
  • P. J. Chan
    • 1
  • M. Y. Chang
    • 1
  • Y. K. Soong
    • 1
  1. 1.The Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyChang Gung Memorial Hospital and Medical School, Linkou Medical CenterTyo-YuanTaiwan 10591 Republic of China

Personalised recommendations