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Comparative effects of secretin (SEC) and cholecystokinin-octapeptide (CCK-8) on pancreatic microcirculation

Digestive Diseases and Sciences volume 40, pages 1199–1206 (1995)Cite this article

Abstract

Using epifluorescent microscopy, we investigated the dynamic changes in pancreatic microcirculationin vivo after bolus administration of secretin (SEC) (0.1–10.0 µg/100 g body wt) and cholecystokinin-octapeptide (CCK-8) (0.005–1.2 µg/100 g body wt) in pentobarbital-anesthetized rats. Pancreatic capillary red cell velocity as a monitor for pancreatic capillary blood flow was measured in 1-min intervals from 2 min prior to 8 min following bolus infusion of SEC or CCK-8. Physiological concentrations of SEC did not increase pancreatic capillary blood flow. However, pharmacological SEC concentrations induced a dose-dependent increase in pancreatic capillary blood flow (to 162±19% of baseline;P<0.05), due to an increase in blood flow velocity (to 153±18% of baseline;P<0.05). In contrast, bolus administration of physiological CCK-8 concentrations, which have been proven to stimulate enzyme secretion, induced a transient and dose-dependent increase in pancreatic capillary blood flow (to 235±24% of baseline;P<0.05), due to an increase in blood flow velocity (to 184±13% of baseline;P<0.05) and capillary diameters (+0.63 ± 0.15 µm;P<0.05).

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Affiliations

  1. From the Department of General Surgery and Department of Trauma Surgery, University of Saarland, 66421, Homburg/Saar, Germany

    T. Plusczyk MD, M. Bauer MD, I. Marzi MD, G. Harbauer MD & G. Feifel MD

  2. Division of Pediatric Surgery, Johns Hopkins University School of Medicine, 21287, Baltimore, Maryland

    T. Plusczyk MD

Authors
  1. T. Plusczyk MD
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  2. M. Bauer MD
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  3. I. Marzi MD
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  4. G. Harbauer MD
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  5. G. Feifel MD
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Plusczyk, T., Bauer, M., Marzi, I. et al. Comparative effects of secretin (SEC) and cholecystokinin-octapeptide (CCK-8) on pancreatic microcirculation. Digest Dis Sci 40, 1199–1206 (1995). https://doi.org/10.1007/BF02065524

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  • DOI: https://doi.org/10.1007/BF02065524

Key words

  • pancreas microcirculation
  • blood flow
  • secretin
  • cholecystokinin
  • epifluorescent microscopy