Digestive Diseases and Sciences

, Volume 40, Issue 4, pp 887–892 | Cite as

Duodenal ulcer

Calcium status in isolated parietal cells
  • Upjeet Kaur
  • Navneet Agnihotri
  • Simran Kaur
  • Madhu Khullar
  • Pradeep Bambery
  • Gagan Sood
  • Kartar Singh
Esophageal, Gastric, And Duodenal Disorders
  • 18 Downloads

Abstract

Although the etiology of duodenal ulcer is not known, its treatment with drugs that reduce acid secretion is well accepted. The central role of calcium in stimulus-secretion coupling resulting in acid secretion by gastric parietal cells is documented. However, the status of intracellular calcium in gastric parietal cells in the basal state in patients with duodenal ulcer is not known. Multiple endoscopic gastric mucosal biopsies from the corpus of the stomach of 52 patients were processed and isolated parietal cells were studied. Intracellular calcium was estimated using fura-2-acetoxymethyl ester. Influx and efflux were determined by using radioactive calcium. Acridine orange retention was used to assess acid production. Only calcium influx at 20 min was significantly (P<0.01) more in patients with duodenal ulcer as compared to the control group. There was no difference between the groups in calcium influx at 0 and 60 min; calcium efflux at 0, 20, and 60 min; intracellular free calcium and acid secretion. We conclude that in the unstimulated state calcium homeostasis in isolated parietal cells of patients with duodenal ulcer shows only a minimal difference as compared to controls.

Key words

duodenal ulcer parietal cells calcium gastric acid 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Upjeet Kaur
    • 1
  • Navneet Agnihotri
    • 1
  • Simran Kaur
    • 1
  • Madhu Khullar
    • 1
  • Pradeep Bambery
    • 1
  • Gagan Sood
    • 1
  • Kartar Singh
    • 1
  1. 1.From the Departments of Experimental MedicineInternal Medicine, and Gastroenterology, Postgraduate Institute of Medical Education & ResearchChandigarhIndia

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