Adaptive cytoprotection in cultured rat gastric mucus-producing cells role of mucus and prostaglandin synthesis
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In cultured gastric mucosal cells, we investigated whether: (1) adaptive cytoprotection was associated with stimulation of endogenous prostaglandin synthesis; (2) prostaglandins given exogenously were cytoprotective against ethanol-induced gastric mucosal cell damage; and (3) a relationship existed between cytoprotection and mucus release. Cytolysis was quantified by measuring51Cr release from prelabeled cells. Mucus release was determined by measurement of [3H]glucosamine release. Concentrations of ethanol >12% caused cell damage and increased51Cr release dose dependently. Pretreatment with low concentrations of ethanol (0.5–1.5%) decreased ethanol-induced51Cr release, but also decreased prostaglandin E2 synthesis. Prostaglandin E2 and 16,16-dimethyl prostaglandin E2 given exogenously were cytoprotective against ethanol-induced gastric mucosal cell damage. Treatment with low concentrations of ethanol (1.5%) increased mucus release from cultured gastric mucosal cells. However, prostaglandin E2 and 16,16-dimethyl prostaglandin E2 did not affect mucus release. We conclude that in cultured gastric mucus-producing cells: (1) adaptive cytoprotection occurs without stimulation of endogenous prostaglandin synthesis but with increase in mucus release; and (2) exogenous prostaglandins are cytoprotective against ethanol-induced gastric mucosal cell damage without stimulating mucus releasein vitro. We postulate that adaptive cytoprotection in cultured gastric mucus-producing cells is not mediated by prostaglandin, but by mucus released in response to a mild irritant.
Key wordsethanol-induced gastric damage mucus prostaglandin adaptive cytoprotection
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- 3.Robert A, Lancaster C, Hanchar AJ, Nezamis JE: Mild irritants prevent gastric necrosis through prostaglandin formation: Histological study. Gastroenterology 74:1086, 1978Google Scholar
- 16.Hiraishi H, Terano A, Ota S, Shiga J, Kobayashi T, Harada H, Ishii M, Ivey KJ, Sugimoto T: Prostaglandin production in cultured gastric mucosal cells: Role of cAMP on its modulation. Prostaglandin 32:259–273, 1986Google Scholar
- 18.Robert A: Cytoprotection and adapted cytoprotection.In Peptic Ulcer Disease: Basic and Clinical Aspects. GF Melis, J Boeve, JJ Misiewicz, (eds). Dordrecht, Martinus Nijhoff, 1985, pp 290–316Google Scholar
- 22.Lacy ER, Ito S: Microscopic analysis of ethanol damage to rat gastric mucosa after treatment with a prostaglandin. 83:619–625, 1982Google Scholar
- 24.Lacy ER: Gastric mucosal resistance to a repeated ethanol insult. Scand J Gastroenterol 20(suppl 110):63–72, 1985Google Scholar