Digestive Diseases and Sciences

, Volume 40, Issue 3, pp 651–660 | Cite as

Cholecystokinin is a potent protective agent against alcohol-induced gastric injury in the rat

Role of endogenous prostaglandins
  • David W. Mercer
  • James M. Cross
  • Jose C. Barreto
  • Nathaniel H. P. Strobel
  • Diane H. Russell
  • Thomas A. Miller
Esophageal, Gastric, And Duodenal Disorders

Abstract

Cholecystokinin is a gastrointestinal hormone known to physiologically regulate pancreatic protein secretion and gallbladder contractility. Some evidence suggests that cholecystokinin is also involved in the maintenance of gastrointestinal mucosal integrity. This study was undertaken to ascertain whether cholecystokinin could prevent the gastric mucosal injury induced by acidified ethanol and what role prostaglandins, and type A and type B cholecystokinin receptors might play in this process. Conscious, fasted rats were given subcutaneous saline or cholecystokinin octapeptide (10–100 µg/kg) 30 min before a l-ml oral gastric bolus of acidified ethanol (150 mM HCl/50% ethanol). Five minutes later, rats were sacrificed and the total area of macroscopic injury quantitated (square millimeters). In additional experiments using a similar protocol, 1 ml of either the cyclooxygenase inhibitor, indomethacin (5 mg/kg), a type A cholecystokinin receptor antagonist, L-364,718 (0.01–1 mg/kg), or the type B cholecystokinin receptor antagonist, L-365,260 (12.5–25 mg/kg) was given intraperitoneally 30 min prior to pretreatment with cholecystokinin octapeptide. Cholecystokinin octapeptide dose-dependently prevented mucosal injury from acidified ethanol (corroborated by histology). The protective effect of cholecystokinin octapeptide was completely negated by L-364,718 and partially reversed by indomethacin, while L-365,260 had no discernible effect in this process. In a further study, cholecystokinin was unable to prevent the damaging effects of aspirin and the inhibition of endogenous prostaglandins. Thus, it appears that cholecystokinin is able to maintain mucosal integrity in the face of a damaging insult by activation of type A cholecystokinin receptors, an effect mediated, at least in part, through the release of endogenous prostaglandins.

Key Words

CCK prostaglandins CCK receptors gastric injury 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • David W. Mercer
    • 1
  • James M. Cross
    • 1
  • Jose C. Barreto
    • 1
  • Nathaniel H. P. Strobel
    • 1
  • Diane H. Russell
    • 1
  • Thomas A. Miller
    • 1
  1. 1.Department of SurgeryUniversity of Texas Medical SchoolHouston

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