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Nonesterified fatty acids in acute cerulein-induced pancreatitis in the rat are they really deleteriousin vivo?

Digestive Diseases and Sciences volume 40, pages 540–545 (1995)Cite this article

Abstract

During acute pancreatitis, experimental data obtainedin vitro suggested that pancreatic lipase generates nonesterified fatty acids (NEFA), noxious for acinar cells, by hydrolysis of pancreatic or circulating triglycerides. The purpose of this work was to determine whether experimentally induced high plasma NEFA levels do indeed aggravatein vivo cerulein-induced pancreatitis. Anesthetized Sprague-Dawley rats received cerulein and were simultaneously infused intravenously with either saline or a triglyceride + heparin mixture (TGH) in order to increase the amount of circulating NEFA. Plasma NEFA increased about fourfold (3.02±0.28 µmol/liter) in animals infused with TGH with respect to controls (0.75±0.05 µmol/liter). In rats receiving cerulein + TGH, pancreatic enzyme levels in plasma, ascites, and histological alterations of the pancreas did not differ from those observed in the rats receiving cerulein + saline. There was less macroscopic pancreatic edema (P<0.01) in the cerulein + TGH group than in the cerulein + saline group. Separate infusion of either heparin alone or of triglycerides alone had no effect. We conclude that high levels of circulating NEFA do not aggravate cerulein pancreatitis in rats and may even induce a protective effect.

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Affiliations

  1. From the INSERM U 410, Biologie et Neuroendocrinologie digestives, Faculté Xavier Bichat, 16 rue Henri Huchard, 75018, Paris

    François Paye MD, Jacques Chariot PhD, Georges Molas MD, Joëlle Benessiano PhD & Claude Rozé MD, PhD

  2. Anatomie Pathologique, Hôpital Beaujon, 100 avenue du Général Leclerc, 92118, Clichy

    François Paye MD, Jacques Chariot PhD, Georges Molas MD, Joëlle Benessiano PhD & Claude Rozé MD, PhD

  3. Biochimie A, CHU Bichat-Claude Bernard, 46 rue Henri Huchard, 75018, Paris, France

    François Paye MD, Jacques Chariot PhD, Georges Molas MD, Joëlle Benessiano PhD & Claude Rozé MD, PhD

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  1. François Paye MD
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  2. Jacques Chariot PhD
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  3. Georges Molas MD
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  5. Claude Rozé MD, PhD
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Additional information

F.P. was the recipient of a scholarship from the Fondation pour la Recherche Médicale (Paris). Partial financial support of the work was provided by the Conseil Scientifique of Faculté X. Bichat and by Association Charles Debray.

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Paye, F., Chariot, J., Molas, G. et al. Nonesterified fatty acids in acute cerulein-induced pancreatitis in the rat are they really deleteriousin vivo?. Digest Dis Sci 40, 540–545 (1995). https://doi.org/10.1007/BF02064365

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  • DOI: https://doi.org/10.1007/BF02064365

Key Words

  • acute pancreatitis
  • cerulein
  • rat
  • fatty acids
  • heparin
  • triglycerides