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Immunocytochemical localization of pancreatitis-associated protein in human small intestine

Digestive Diseases and Sciences volume 40, pages 519–524 (1995)Cite this article

Abstract

Pancreatitis-associated protein (PAP) is a lectin-related protein barely detectable in normal pancreas but overexpressed by this tissue during the acute phase of the pancreatitis. We describe in this report that PAP is constitutively expressed in the human intestinal tract. Northern blot analysis with pancreatic cDNA as probe shows the presence of a transcript in the jejunum that has the same electrophoretic mobility as the pancreatic mRNA. No signal was detected in colon, however. In addition, immunoblotting assays, utilizing specific rabbit immunosera prepared against PAP, revealed the presence of a protein of 16,000 Da (as in pancreatic juice) in the homogenate of jejunum, but not of the colon. When the same antibodies were used for tissule localization of the protein, positive immunoreactivity was observed on Paneth cells and in some goblet cells located in jejunum at the bottom of the crypts. No staining was observed in colon.

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Author information

Affiliations

  1. U 315 INSERM, 46 Bd. de la Gaye, F-13009, Marseille, France

    Luis Masciotra PhD, Paulette Lechêne de la Porte PhD, Jean-Marc Frigerio PhD, Nelson J. Dusetti PhD, Jean-Charles Dagorn PhD & Juan L. Iovanna MD, PhD

  2. Department of Physiology, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina

    Luis Masciotra PhD, Paulette Lechêne de la Porte PhD, Jean-Marc Frigerio PhD, Nelson J. Dusetti PhD, Jean-Charles Dagorn PhD & Juan L. Iovanna MD, PhD

  3. U.130 Institut National de la Santé et de la Recherche Médicale, F-13009, Marseille, France

    Luis Masciotra PhD, Paulette Lechêne de la Porte PhD, Jean-Marc Frigerio PhD, Nelson J. Dusetti PhD, Jean-Charles Dagorn PhD & Juan L. Iovanna MD, PhD

Authors
  1. Luis Masciotra PhD
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  2. Paulette Lechêne de la Porte PhD
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  3. Jean-Marc Frigerio PhD
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  4. Nelson J. Dusetti PhD
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  5. Jean-Charles Dagorn PhD
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  6. Juan L. Iovanna MD, PhD
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Additional information

This work was supported by the French-Argentinian Cooperation Program (INSERM-CONICET). J.-M.F. is supported, in part, by a fellowship from Jouveinal. N.J.D. is supported by a fellowship from the Fondation pour la Recherche Médicale (FRM).

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Masciotra, L., Lechêne de la Porte, P., Frigerio, JM. et al. Immunocytochemical localization of pancreatitis-associated protein in human small intestine. Digest Dis Sci 40, 519–524 (1995). https://doi.org/10.1007/BF02064359

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  • DOI: https://doi.org/10.1007/BF02064359

Key Words

  • pancreatitis-associated protein
  • Paneth cells
  • goblet cells
  • human
  • intestine
  • immunocytochemistry