Digestive Diseases and Sciences

, Volume 40, Issue 5, pp 1132–1139 | Cite as

Roles of hepatocyte growth factor and its receptor in gastric mucosa

A cell biological and molecular biological study
  • Shingo Tsuji
  • Sunao Kawano
  • Masahiko Tsujii
  • Hideyuki Fusamoto
  • Takenobu Kamada
Esophageal, Gastric, And Duodenal Disorders

Abstract

Hepatocyte growth factor (HGF) stimulates the growth of hepatocytes and other epithelial cells. A gene for the HGF receptor,c-met, is detected in the intestinal tract and the liver, as well as in gastric carcinoma cells. However, the role of HGF in the regeneration of the normal gastric mucosa is not known. The purpose of the present study was to elucidate the effects of HGF on the morphogenesis of cultured gastric mucosal cells and to evaluate the role of HGF andc-met in the healing process in rat gastric mucosa. The cultured gastric mucosal cells developed a branching morphology in a collagen matrix supplemented with HGF or fetal calf serum. They did not form this morphology on a plastic dish or in the collagen without HGF or the serum. In anin vivo study, total RNA was extracted from rat gastric mucosa 6, 24, 48, and 96 hr after the exposure to a solution of 0.6 M HCl. HGF messenger RNA was not detected, butc-met was expressed in the mucosa. The increased expression ofc-met was followed by healing of the mucosal injury. These results indicate that HGF plays important roles in the morphogenesis of gastric mucosal cells and that the HGF receptor gene participates in the healing process of gastric mucosa.

Key words

hepatocyte growth factor c-met rat stomach morphogenesis 

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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Shingo Tsuji
    • 1
  • Sunao Kawano
    • 1
  • Masahiko Tsujii
    • 1
  • Hideyuki Fusamoto
    • 1
  • Takenobu Kamada
    • 1
  1. 1.First Department of MedicineOsaka UniversitySuitaJapan

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