Ileal release of glucagon-like peptide-1 (GLP-1)
- 171 Downloads
There is evidence that the distal intestine participates in the regulation of gastric motor and secretory function. It was the aim of this study to examine in greater detail the effects of ileal nutrient exposure on human gastric acid secretion and to investigate potential intermediary mechanisms. Twelve normal subjects were intubated with an oroileal multilumen tube assembly for gastric, duodenal, and ileal perfusion of marker and test solutions, aspiration, and intestinal manometry. We studied ileal effects on gastric acid output in the unstimulated, interdigestive state (during early phase II,N=6), and during endogenous stimulation by intraduodenal essential amino acid perfusion,N=6) and on release of candidate humoral mediators, peptide YY (PYY) and glucagonlike peptide-1 (GLP-1), both known inhibitors of human gastric acid secretion. Compared with ileal saline perfusion, ileal carbohydrate (total caloric load: 60 kcal) decreased interdigestive gastric acid output by 64% (P<0.01), and endogenously stimulated output by 68%, respectively (P<0.005). Under all experimental conditions, ileal carbohydrate increased plasma GLP-1 by 80–100% (allP<0.005). Ileal lipid perfusion had similar inhibitory effects on gastric acid output and stimulatory effects on GLP-1 release as had ileal carbohydrate. By contrast, ileal perfusion with peptone had no or only weak effects on either acid output or plasma GLP-1. Plasma PYY concentrations and suppression of gastric secretion in response to ileal perfusions were not correlated. In humans, both interdigestive and endogenously stimulated gastric acid output are inhibited in response to intraileal carbohydrate or lipids, but not protein, Decreased acid output is associated with release of GLP-1, but not PYY. These findings support the hypothesis that the distal small intestine may participate in the late postprandial inhibitory regulation of gastric secretory function in humans and that GLP-1 may be an intermediary factor.
Key wordscarbohydrate lipid glucagon-like peptide-1 human interdigestive peptide YY protein postprandial gastric acid secretion
Unable to display preview. Download preview PDF.
- 4.Read NW, McFarlane A, Kinsman RJ, Bates TE, Blackhall NW, Farrar GBJ, Hall JC, Moss G, Morris AP, O'Neill B, Welch I, Lee Y, Bloom SR: Effect of infusion of nutrient solutions into the ileum on gastrointestinal transit and plasma levels of neurotensin and enteroglucagon. Gastroenterology 86:274–280, 1984PubMedGoogle Scholar
- 5.Spiller RC, Trotman IF, Adrian TE, Bloom SR, Misiewicz JJ, Silk DBA: Further characterisation of the “ileal brake” reflex in man—effect of ileal infusion of partial digests of fat, protein, and starch on jejunal motility and release of neurotensin, enteroglucagon, and peptide YY. Gut 29:1042–1051, 1988PubMedGoogle Scholar
- 12.Soon-Shiong P, Debas HT, Seal AM, et al: Colonic inhibition of gastric acid secretion in man. Surg Forum 31:152–154, 1980Google Scholar
- 25.Holst JJ, Bersani M: Assays for peptide products of somatostatin gene expression. In Methods in Neurosciences, Vol 5: Neuropeptide Technology. P. Michael Conn (Ed). San Diego, Academic Press, 1991, pp 3–22Google Scholar
- 28.Box GEP, Hunter WS, Hunter JS: Statistics for Experimenters. New York, John Wiley & Sons, 1978Google Scholar
- 47.Wettergren A, Schjoldager B, Mortensen PE, Myhre J, Christiansen J, Holst JJ: Truncated glucagon-like peptide-1 (proglucagon 78–107 amide) inhibits gastric and pancreatic functions in man. Dig Dis Sci (in press).Google Scholar