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Role of SPan-1 antigen in adhesion of human colon cancer cells to vascular endothelium

  • Gastrointestinal Oncology
  • Published:
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Abstract

Recently E-selectin (ELAM-1, endothelial leukocyte adhesion molecule-1) was shown to recognize not only sialyl Lewis X but also sialyl Lewis A, and these carbohydrate antigens may be involved in the process of the adhesion between cancer cells and endothelial cells in cancer metastasis. To investigate the contribution of sialylated carbohydrate antigen, SPan-1, and sialic acid to the adhesion of human colon cancer cells to endothelial cells, adhesion assay using HUVECs (human umbilical vein endothelial cells) was performed. The adhesion was significantly inhibited by pretreatment with anti-E-selectin antibody, indicating that this adhesion was thought to be mediated by E-selectin. When these cancer cells were pretreated with SPan-1 antibody, the adhesion was significantly inhibited in a concentration-dependent manner. The adhesion was also inhibited by pretreatment with neuraminidase. These findings suggest that the SPan-1 antigen plays a significant role in the adhesion of human colon cancer cells to endothelial cells, and sialylation of the terminal structure of carbohydrate antigens is important in this adhesion.

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Authors and Affiliations

  1. From the First Department of Surgery, Osaka City University, Medical School Abeno-ku, 545, Osaka, Japan

    Nobuya Yamada MD, Yong-Suk Chung MD, Tetsuji Sawada MD, Masahiro Okuno MD & Michio Sowa MD, FACS

Authors
  1. Nobuya Yamada MD
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  2. Yong-Suk Chung MD
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  3. Tetsuji Sawada MD
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  4. Masahiro Okuno MD
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  5. Michio Sowa MD, FACS
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Yamada, N., Chung, YS., Sawada, T. et al. Role of SPan-1 antigen in adhesion of human colon cancer cells to vascular endothelium. Digest Dis Sci 40, 1005–1012 (1995). https://doi.org/10.1007/BF02064189

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  • DOI: https://doi.org/10.1007/BF02064189

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