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Passive elastic wall properties in isolated guinea pig small intestine

  • Intestinal Disorders, Inflammatory Bowel Disease, Immunology, And Microbiology
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Abstract

The aim was to study and compare the passive biomechanical wall properties in the isolated duodenum and distal ileum of the guinea pigin vitro. The organ bath contained a Krebs-Ringer solution with 10−2 M MgCl2 to abolish smooth muscle contractile activity. Stepwise inflation of an intraluminal balloon, in which the cross-sectional area (CSA) was measured, provided the distension stimulus. The circumferential wall tension-strain distributions and wall stiffness-strain relations were computed from steady-state values of these measurements in order to evaluate the passive elastic properties. The CSA always reached equilibrium within the 2-min distension period. The CSAs obtained in the distal ileum were higher than those in the duodenum (P<0.001). The basal CSA was 17.31±1.14 mm2 and 12.96±0.42 mm2 for the distal ileum and the duodenum, respectively (P<0.01). At a maximum pressure of 6 kPa, the CSA of the ileum was 56.63±1.81 mm2 and 36.86±1.76 mm2 for the duodenum (P<0.01). The circumferential wall tension-strain distributions showed an exponential behavior that accorded well with the equationY=exp(a+bX) with determination coefficients of 0.96±0.01 and 0.99±0.00 in the duodenal segments in the distal ileal segments, respectively. The values ofa (intercept with they-axis) were 0.54±0.11 and −0.35±0.19 for the duodenal and ileal segments, respectively (P<0.001). The slope of the curves (b values) were 4.34±0.35 in the duodenal and 5.23±0.37 in the ileal segments (0.1>P>0.05). In conclusion, differences in elastic properties were found between the proximal and distal small intestine.

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References

  1. Bueno L, Fioramonti J, Ruckebusch Y: Rate of flow of digesta and electrical activity of the small intestine in dogs and sheep. J Physiol 292:16–26, 1975

    Google Scholar 

  2. Gabella G: On the musculature of the gastro-intestinal tract of the guinea-pig. Anat Embryol 163:135–156, 1981

    Google Scholar 

  3. Kellow J, Borody T, Phillips S, Tucker R, Haddad A: Human interdigestive motility: Variations in patterns from esophagus to colon. Gastroenterology 91:386–395, 1986

    PubMed  Google Scholar 

  4. Schulze-Delrieu K: Intrinsic differences in the filling response of the guinea-pig duodenum and ileum. J Lab Clin Med 117:44–50, 1991

    PubMed  Google Scholar 

  5. Weems WA, Seygal GE: Fluid propulsion by cat intestinal segments under conditions requiring hydrostatic work. Am J Physiol 240:G147-G156, 1981

    PubMed  Google Scholar 

  6. Quigley EMM, Borody TJ, Philips SF, Weinbeck M, Tucker RL, Haddad A: Motility of the terminal ileum and ileocecal sphincter in healthy humans. Gastroenterology 87:857–866, 1984

    PubMed  Google Scholar 

  7. Quigley EMM, Philips SF, Dent J: Distinctive patterns of interdigestive motility at the canine ileocolonic junction. Gastroenterology 87:836–844, 1984

    PubMed  Google Scholar 

  8. Brown NJ, Read NW, Richardson A, Ramsey RDE, Borgentoft C: Characteristics of lipid substances activating the ileal brake in the rat. Gut 31:1126–1129, 1990

    PubMed  Google Scholar 

  9. Soper NJ, Chapman NJ, Kelly KA, Brown ML, Philips SF, Go VLW: The ileal brake after ileal pouch-anal anastomosis. Gastroenterology 98:111–116, 1990

    PubMed  Google Scholar 

  10. Spiller RC, Trotman IF, Higgins BE, Ghatei MA, Grimble GK, Lee YC, Bloom SR, Misieiicz JJ, Silk DBA: The ileal brake—inhibition of jejunal motility after ileal fat perfusion in man. Gut 25:365–374, 1984

    PubMed  Google Scholar 

  11. Dobrin PB: Mechanical properties of arteries. Physiol Rev 58:397–460, 1978

    PubMed  Google Scholar 

  12. Elbrønd H, Tøttrup A, Forman A: Mechanical properties of isolated smooth muscle from rabbit sphincter of oddi and duodenum. Scand J Gastroenterol 26:289–294, 1991

    PubMed  Google Scholar 

  13. Meiss RA: Some mechanical properties of cat intestinal muscle. Am J Physiol 220:2000–2007, 1971

    PubMed  Google Scholar 

  14. Fung YC: Stress strain history relations of soft tissue in simple elongation.In Biomechanics. Its Foundations and Objectives. YC Fung, N Perrone, M Anliker (eds). Englewood Cliffs, New Jersey, Prentice Hall, 1972

    Google Scholar 

  15. Bergel DH: The properties of blood vessels.In Biomechanics. Its Foundations and Objectives. YC Fung, N Perrone, M Anliker (eds). Englewood Cliffs, New Jersey, Prentice Hall, 1972

    Google Scholar 

  16. Gregersen H, Kraglund K, Djurhuus JC: Variations in duodenal cross-sectional area during the interdigestive motility complex. Am J Physiol 259:G26-G31, 1990

    PubMed  Google Scholar 

  17. Gregersen H, Stødkilde-Jørgensen H, Djurhuus JC, Mortensen SO: The four electrode impedance technique: A method for investigation of compliance in luminal organs. Clin Phys Physiol Meas 9(suppl A):61–64, 1988

    PubMed  Google Scholar 

  18. Gregersen H, Andersen MB: Impedance measuring system for quantification of cross-sectional area in the gastrointestinal tract. Med Biol Eng Comput 29:108–110, 1991

    PubMed  Google Scholar 

  19. Gregersen H, Jensen LS, Djurhuus JC: Changes in oesophagcal wall biomechanics after portal vein banding and variceal sclerotherapy measured by a new technique. An experimental study in rabbits. Gut 29:1699–1704, 1988

    PubMed  Google Scholar 

  20. Storkholm JH, Jørgensen CS, Dall FH, Jensen SL, Gregersen H: Differences exist in passive elastic wall properties between segments of isolated guinea-pig distal ileum and duodenumin vitro. Neurogastroenterol Motil 6:21–27, 1994

    Google Scholar 

  21. Lose G, Colstrup H, Saksager K, Kristensen JK: A new probe for measurement of related values of cross-sectional area and pressure in a biological tube. Med Biol Eng Comput 24:488–492, 1986

    PubMed  Google Scholar 

  22. Harris JH, Therkelsen EE, Zinner NR: Electrical measurement of ureteral flow:In Urodynamics. S Boyarsky, EA Tanagho, CW Gottschalk, PD Zimskind (eds). London, Academic Press, 1971

    Google Scholar 

  23. Gregersen H, Jørgensen CS, Dall FH: Biomechanical wall properties in the isolated perfused porcine duodenum. An experimental study using impedance planimetry. J Gastrointest Motil 4:125–135, 1992

    Google Scholar 

  24. Fung YC: Biomechanics: Mechanical Properties of Living Tissues. New York, Springer Verlag, 1981

    Google Scholar 

  25. Gregersen H, Jørgensen CS, Dall FH, Jensen SL: Characteristics of spontaneous and evoked motility in the isolated perfused porcine duodenum. J Appl Physiol 73:9–19, 1992

    PubMed  Google Scholar 

  26. Gordon AR, Siegman MJ: Mechanical properties of smooth muscle. 1. Length-tension and force-velocity relations. Am J Physiol 221:1243–1249, 1971

    PubMed  Google Scholar 

  27. Bagshaw RV, Attinger FML: Two directional delayed compliance in the canine abdominal aorta. Experientia 28:803–804, 1972

    PubMed  Google Scholar 

  28. Baumbach GL, Heistad DD: Remodeling of cerebral arteries in chronic hypertension. Hypertension 13:968–972, 1989

    PubMed  Google Scholar 

  29. Arhan P, Faverdin C, Persoz B, Devroede G, Dubois F, Dornic C, Pellerin D: Relationship between viscoelastic properties of the rectum and anal pressure in man. J Appl Physiol 41:677–682, 1976

    PubMed  Google Scholar 

  30. Madoff RD, Orrom WJ, Rothenberger DA, Goldberg SM: Rectal compliance: A critical reappraisal. Int J Colorect Dis 5:37–40, 1990

    Google Scholar 

  31. Gabella G: Structure of muscles and nerves in the gastrointestinal tract.In Physiology of the Gastrointestinal Tract. LR Johnson, J Christensen, MJ Jackson, ED Jacobson, JH Walsh (eds). New York, Raven Press, 1987

    Google Scholar 

  32. Shirazi S, Schulze-Delrieu K, Brown CK: Duodenal resistance to the emptying of various solutions from the isolated cat stomach. J Lab Clin Med 111:654–660, 1988

    PubMed  Google Scholar 

  33. Macagno EO, Christensen J: Fluid mechanics of the duodenum. Annu Rev Fluid Mech 12:139–158, 1980

    Google Scholar 

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Authors and Affiliations

  1. From the Core Center of Gastrointestinal Motility and Biomechanics, Denmark

    J. H. Storkholm MD, G. E. Villadsen MD, S. L. Jensen PhD & H. Gregersen MD

  2. Departments of Surgical Gastroenterology L and Gastroenterology-Hepatology V, Section AKH, Denmark

    J. H. Storkholm MD, G. E. Villadsen MD, S. L. Jensen PhD & H. Gregersen MD

  3. Institute of Experimental Clinical Research, Aarhus University Hospital, Skejby Sygehus, DK-8200, Aarhus N, Denmark

    J. H. Storkholm MD, G. E. Villadsen MD, S. L. Jensen PhD & H. Gregersen MD

Authors
  1. J. H. Storkholm MD
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  2. G. E. Villadsen MD
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  3. S. L. Jensen PhD
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  4. H. Gregersen MD
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Storkholm, J.H., Villadsen, G.E., Jensen, S.L. et al. Passive elastic wall properties in isolated guinea pig small intestine. Digest Dis Sci 40, 976–982 (1995). https://doi.org/10.1007/BF02064185

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  • DOI: https://doi.org/10.1007/BF02064185

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