Digestive Diseases and Sciences

, Volume 40, Issue 1, pp 96–99 | Cite as

Effect of capsaicin on release of substance P-like immunoreactivity from vascularly perfused rat duodenum

  • Mineko Fujimiya
  • Yin Nam Kwok
Motility, Nerve-Gut Interactions, Hormones, And Receptors


The release of substance P-like immunoreactivity (SPLI) from the rat duodenum was investigated using anin situ vascularly perfused preparation. Results show that the basal release of SPLI was measurable and significantly enhanced by the administration of both 1, 3, and 10 µM of capsaicin, indicating that SPLI is present in capsaicin-sensitive afferent nerve fibers. It is concluded that the present model may be useful for the study of the control of duodenal release of SPLI.

Key words

rat duodenum substance P-like immunoreactivity capsaicin 


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  1. 1.
    Bartho L, Holzer P: Search for a physiological role of substance P in gastrointestinal motility. Neuroscience 16:1–32, 1985PubMedGoogle Scholar
  2. 2.
    Costa M, Furness JB, Llewellyn-Smith IJ, Cuello AC: Projection of substance P-containing neurons within the guinea-pig small intestine. Neuroscience 6:411–424, 1981PubMedGoogle Scholar
  3. 3.
    Buck SH, Burks TF: The neuropharmacology of capsaicin: Review of some recent observation. Pharmacol Rev 38:179–226, 1986PubMedGoogle Scholar
  4. 4.
    Holzer P, Schluet W, Lippe IT, Sametz W: Involvement of capsaicin-sensitive sensory neurons in gastrointestinal function. Acta Physiol Hung 69:403–411, 1987PubMedGoogle Scholar
  5. 5.
    Maggi CA, Santicioli P, Manzini S, Meli A: Capsaicin activates neurogenic non-adrenergic non-cholinergic relaxations of the isolated rat duodenum. Eur J Pharmacol 120:367–370, 1986PubMedGoogle Scholar
  6. 6.
    Holzer P, Sametz W: Gastric mucosal protection against ulcerogenic factors in the rat mediated by capsaicin-sensitive afferent neurons. Gastroenterology 91:975–981, 1986PubMedGoogle Scholar
  7. 7.
    Maggi CA, Evangelista S, Abelli L, Somma V, Meli A: Capsaicin-sensitive mechanisms and experimentally induced duodenal ulcers in rats. J Pharm Pharmacol 39:559–561, 1987PubMedGoogle Scholar
  8. 8.
    Bartho L, Petho G, Antal A, Holzer P, Szolcsanyi J: Two types of relaxation due to capsaicin in the guinea pig isolated ileum. Neurosci Lett 81:146–150, 1987PubMedGoogle Scholar
  9. 9.
    Mayer EA, Koelbel CBM, Snape WJ, Eysselein V, Ennes H, Kodner A: Substance P and CGRP mediate motor response of rabbit colon to capsaicin. Am J Physiol 259:G889-G897, 1990PubMedGoogle Scholar
  10. 10.
    Holzer P: Local effector functions of capsaicin-sensitive sensory nerve endings: Involvement of tachykinins, calcitonin gene-related peptide and other neuropeptides. Neuroscience 24:739–768, 1988PubMedGoogle Scholar
  11. 11.
    Holzer P, Guth PH: Neuropeptide control of rat gastric mucosal blood flow. Circ Res 68:100–105, 1991PubMedGoogle Scholar
  12. 12.
    Karmeli F, Eliakim R, Okon E, Rachmilewitz D: Gastric mucosal damage by ethanol is mediated by substance P and prevented by ketotifen, a mast cell stabilizer. Gastroenterology 100:1206–1216, 1991PubMedGoogle Scholar
  13. 13.
    Kwok YN, McIntosh CHS: Release of substance P-like immunoreactivity from the vascularly perfused rat stomach. Eur J Pharmacol 180:201–207, 1990PubMedGoogle Scholar
  14. 14.
    Donnerer J, Bartho L, Holzer P, Lembeck F: Intestinal peristalsis associated with release of immunoreactive substance P. Neuroscience 11:913–918, 1984PubMedGoogle Scholar
  15. 15.
    Fujimiya M, McIntosh CHS, Kimura H, Kwok YN: Effect of carbachol on luminal release of somatostatin from isolated perfused rat duodenum. Neurosci Lett 145:229–233, 1992PubMedGoogle Scholar
  16. 16.
    Renzi D, Santicioli P, Maggi CA, Surrenti C, Pradelles P, Meli A: Capsaicin-induced release of substance P-like immunoreactivity from the guinea pig stomachin vitro andin vivo. Neurosci Lett 92:254–258, 1988PubMedGoogle Scholar
  17. 17.
    Maggi CA, Santicioli P, Geppetti P, Patacchini R, Frilli S, Astolfi M, Fusco B, Meli A: Simultaneous release of substance P- and calcitonin gene-related peptide (CGRP)-like immunoreactivity from isolated muscle of the guinea pig urinary bladder. Neurosci Lett 87:163–167, 1988PubMedGoogle Scholar
  18. 18.
    Renzi D, Evangelista S, Mantellini P, Santicioli P, Maggi CA, Geppetti P, Surrenti C: Capsaicin-induced release of neurokinin A from muscle and mucosa of gastric corpus: correlation with capsaicin-evoked release of calcitonin gene-related peptide. Neuropeptides 19:137–145, 1991PubMedGoogle Scholar
  19. 19.
    Holzer-Petsche U, Seitz H, Lembeck F: Effect of capsaicin on gastric corpus smooth muscle of the rat in vitro. Eur J Pharmacol 162:29–36, 1989PubMedGoogle Scholar
  20. 20.
    Esplugues JV, Ramos EG, Gil L, Esplugues J: Influence of capsaicin-sensitive afferent neurons on the acid secretory responses of the rat stomachin vivo. Br J Pharmacol 100:491–496, 1990PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Mineko Fujimiya
    • 1
    • 2
  • Yin Nam Kwok
    • 1
    • 2
  1. 1.From the Department of AnatomyShiga University of Medical ScienceOtsu, ShigaJapan
  2. 2.Department of PhysiologyUniversity of British ColumbiaVancouverCanada

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