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Digestive Diseases and Sciences

, Volume 40, Issue 11, pp 2312–2316 | Cite as

Role of dorsal motor nucleus of vagus in gastric function and mucosal damage induced by ethanol in rats

  • Y. S. Chan
  • J. K. S. Ko
  • C. H. Cho
Esophageal, Gastric, And Duodenal Disorders

Abstract

Experimental evidence indicates that the autonomic nervous system, especially the cholinergic pathway, modulates the mucosal defensive mechanism and affects mucosal damage in the stomach. The present study investigated the role of the dorsal motor nucleus of vagus (DMV) in gastric function and its influences on ethanol-induced mucosal damage in pentobarbitone-anesthetized rats. Electrolytic lesion of the DMV as compared with sham operation and lesions of other brain areas, eg, nucleus reticular gigantocellularis and cuneate nucleus, reduced the basal gastric mucosal blood flow (GMBF) and also the blood flow after ethanol administration. The same operation did not affect the acid secretion either in the basal state or during the ethanol treatment period. Lesions at the caudal half of the DMV produced a bigger depression of GMBF when compared with lesion at the rostral half. In the sham-operated rats, ethanol induced severe hemorrhagic lesions in the gastric glandular mucosa, and this was significantly potentiated by lesions at the DMV, especially in the caudal half. The present findings indicate that acute DMV damage at the caudal half markedly affects the GMBF but not the acid secretion. The action on GMBF may contribute to the aggravation of ethanol-induced gastric damage in rats. These data reinforce the idea that the central vagal pathway, especially the caudal half of the DMV, plays a significant role in the modulation of GMBF, which in turn affects the integrity of gastric mucosal barrier.

Key words

dorsal motor nucleus of vagus ethanol gastric mucosal blood flow gastric acid secretion gastric damage 

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References

  1. 1.
    Shapiro RE, Miselis RR: The central organization of the vagus nerve innervating the stomach of the rat. J Comp Neurol 238:473–488, 1985PubMedGoogle Scholar
  2. 2.
    Leslie RA, Gwyn DG, Hopkins DA: The central distribution of the cervical vagus nerve and gastric afferent and efferent projections in the rat. Brain Res 8:37–43, 1982Google Scholar
  3. 3.
    Cho CH, Chen BW, Hui WM, Lam SK, Ogle CW: The role of the vagus nerve in the protective action of acid inhibitors on ethanol-induced gastric mucosal damage in rats. J Gastroenterol Hepatol 7:178–183, 1992PubMedGoogle Scholar
  4. 4.
    Glavin GB, Szabo S: Effect of vagotomy and adrenalectomy on gastroprotection by calcium chelators. Dig Dis Sci 35:1017, 1990Google Scholar
  5. 5.
    Miller TA: Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol 245:G601-G623, 1985Google Scholar
  6. 6.
    Mozsik GY, Moron F, Javor T: Cellular mechanisms of the development of gastric mucosal damage and of gastrocytoprotection induced by prostacyclin in rats. A pharmacological study. Prostaglandins Leukotrieues Med 9:71–84, 1982Google Scholar
  7. 7.
    Poon YK, Cho CH, Ogle CW: Sub-diaphragmatic vagotomy attenuates the protective action of paracetamol against ethanol-induced gastric damage in rats. Med Sci Res 16:1285, 1988Google Scholar
  8. 8.
    Taché T, Stephens RL, Ishikawa T: Central nervous system action of TRH to influence gastrointestinai function and ulceration. Ann NY Acad Sci 553:269–285, 1989PubMedGoogle Scholar
  9. 9.
    Thiefin G, Taché Y, Leung FW, Guth PH: Central nervous system action of thyrothropin-releasing hormone to increase gastric mucosal blood flow in the rat. Gastroenterology 97:405–411, 1989PubMedGoogle Scholar
  10. 10.
    Yoneda M, Taché Y: Vagal regulation of gastric prostaglandin E2 release by central TRH in rats. Am J Physiol 264:G231-G236, 1993PubMedGoogle Scholar
  11. 11.
    Henegan JM, Seidel ER, Miller TA: Evidence that cytoprotection may be vagally mediated. Gastroenterology 84:1186, 1983Google Scholar
  12. 12.
    Cho CH, Ko JK, Tang XL: The differential mechanisms of mild irritants on adaptive cytoprotection. J Gastroenterol Hepatol Suppl 1:S24-S28, 1994Google Scholar
  13. 13.
    Paxinos G, Watson C: The Rat Brain in Stereotopic Coordinates, 2nd ed. Sydney, Academic Press, 1986Google Scholar
  14. 14.
    Mersereau WWA, Hinchey EJ: Effect of gastric acidity on gastric ulceration induced by hemorrhage in the rat, utilizing chamber technique. Gastroenterology 64:1130–1135, 1973PubMedGoogle Scholar
  15. 15.
    Wong SH, Cho CH, Ogle CW: Protection by zinc sulphate against ethanol-induced mucosal barrier. Pharmacology 33:94–102, 1986PubMedGoogle Scholar
  16. 16.
    Shephred AD, Riedel GL: Continuous measurement of intestinal mucosal blood flow by laser-Doppler velocimetry. Am J Physiol 242:G668-G672, 1982PubMedGoogle Scholar
  17. 17.
    Pagani FD, Norman WP, Kasbekar DK, Gillis RA: Localization of sites within dorsal motor nucleus of vagus that affect gastric motility. Am J Physiol 249:G73-G84, 1985PubMedGoogle Scholar
  18. 18.
    Cho CH, Ogle CW, Dai S: Vagal-induced gastric ulceration and zinc pretreatment in rats: some preliminary observations. IRCS Med Sci 4:469, 1976Google Scholar
  19. 19.
    Laughton WB, Powley TL: Localization of efferent function in the dorsal motor nucleus of the vagus. Am J Physiol 252:R13-R25, 1987PubMedGoogle Scholar
  20. 20.
    Wyrwicka W, Garcia R: Effect of electrical stimulation of the dorsal motor nucleus of the vagus on gastric acid secretion in cats. Exp Neurol 65:315–325, 1979PubMedGoogle Scholar
  21. 21.
    Kerr FWL, Preshaw RM: Secretomotor function of the dorsal motor nucleus of the vagus. J Physiol 205:405–415, 1969PubMedGoogle Scholar
  22. 22.
    Stephens RL Jr, Yang H, Rivier J, Taché Y: Intracisternal injection of CRF antagonist blocks surgical stress—induced inhibition of gastric secretion in the rat. Peptides 9:1067–1070, 1988PubMedGoogle Scholar
  23. 23.
    Cho CH: The inhibitory action of ethanol on the gastric mucosal and its interaction with the vagus in rats. Agents Actions 35:135–139, 1992PubMedGoogle Scholar
  24. 24.
    Cho CH, Chen BW, Hui WM, Lam SK: The influence of acute or chronic nicotine treatment on ethanol-induced gastric mucosal damage in rats. Dig Dis Sci 35:106–112, 1990PubMedGoogle Scholar
  25. 25.
    Guth PH: Gastric blood flow in ethanol injury and prostaglandin cytoprotection. Scand J Gastroenterol 21(suppl 125):86–91, 1986Google Scholar
  26. 26.
    Coceani F, Pace-Asciak C, Volta F, Wolfe LS: Effect of nerve stimulation on prostaglandin formation and release from the rat stomach. Am J Physiol 213:1056–1065, 1967PubMedGoogle Scholar
  27. 27.
    Singh J: Prostaglandin release from rat stomach following vagal stimulation or administration of acetylcholine. Eur J Pharmacol 65:39–48, 1980PubMedGoogle Scholar
  28. 28.
    Robert A: Cytoprotection by prostaglandins. Gastroenterology 77:61–67, 1979PubMedGoogle Scholar
  29. 29.
    Miller TA, Jacobson ED: Gastrointestinal cytoprotection by prostaglandins. Gut 20:75–87, 1979PubMedGoogle Scholar
  30. 30.
    Kiraly A, Suto G, Livingston EH, Guth P, Pierre S ST, Taché Y: Central vagal activation by TRH induces gastric hyperemia: Role of CGRP in capsaicin-sensitive afferents in rats. Am J Physiol 267:G1041-G1049, 1994PubMedGoogle Scholar
  31. 31.
    Tanaka T, Guth P, Taché Y: Role of intric oxide in gastric hyperemia induced by central vagal stimulation. Am J Physiol 264:G280-G284, 1993PubMedGoogle Scholar
  32. 32.
    Takayama K, Ishikawa N, Muira M: Sites of origin and termination of gastric preganglionie neurons: an HRP study in the rat. J Auton Nerv Syst 6:211–223, 1982PubMedGoogle Scholar
  33. 33.
    Bieger D, Hopkins DA: Viscerotopic representation of the upper alimentary tract in the medulla oblongata in the rat: the nucleus ambiguus. J Comp Neurol 262:546–562, 1987PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Y. S. Chan
    • 1
  • J. K. S. Ko
    • 1
  • C. H. Cho
    • 1
  1. 1.From the Departments of Physiology and Pharmacology, Faculty of MedicineThe University of Hong KongHong Kong

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