Antonie van Leeuwenhoek

, Volume 15, Issue 1, pp 34–36 | Cite as

Failure to cultivate rabies virus in vitro

  • J. D. Verlinde
  • A. Kret


Report of the unsuccessful cultivation of rabies fixed virus in a cell-free medium consisting of steamed sheep-brain extract, glycine, peptone and a number of accessory factors (tryptophan, aneurine, nicotinic acid amide, pyridoxine hydrochloride, sodium pantothenate and lactoflavin). After 24 and 48 hour's incubation at 37° C, under aerobic conditions, the virus had completely lost its virulence, and did not induce immunity to reinoculation with active virus.


Tryptophan Peptone Lactoflavin Nicotinic Acid Aerobic Condition 
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    N. Veeraraghavan, Ind. Journ. Med. Res.34, 207, 225, 1946.Google Scholar
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    N. Veeraraghavan, Ind. Journ. Med. Res.35, 237, 1947.Google Scholar
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    N.Veeraraghavan, Nature 782, 1947.Google Scholar

Copyright information

© Swets en Zeitlinger 1949

Authors and Affiliations

  • J. D. Verlinde
    • 1
  • A. Kret
    • 1
  1. 1.Department of Bacteriology and Experimental Pathology of the Institute for Preventive MedicineLeiden

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