Antonie van Leeuwenhoek

, Volume 15, Issue 1, pp 34–36 | Cite as

Failure to cultivate rabies virus in vitro

  • J. D. Verlinde
  • A. Kret
Article
  • 12 Downloads

Summary

Report of the unsuccessful cultivation of rabies fixed virus in a cell-free medium consisting of steamed sheep-brain extract, glycine, peptone and a number of accessory factors (tryptophan, aneurine, nicotinic acid amide, pyridoxine hydrochloride, sodium pantothenate and lactoflavin). After 24 and 48 hour's incubation at 37° C, under aerobic conditions, the virus had completely lost its virulence, and did not induce immunity to reinoculation with active virus.

Keywords

Tryptophan Peptone Lactoflavin Nicotinic Acid Aerobic Condition 

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References

  1. 1.
    N. Veeraraghavan, Ind. Journ. Med. Res.34, 207, 225, 1946.Google Scholar
  2. 2.
    N. Veeraraghavan, Ind. Journ. Med. Res.35, 237, 1947.Google Scholar
  3. 3.
    N.Veeraraghavan, Nature 782, 1947.Google Scholar

Copyright information

© Swets en Zeitlinger 1949

Authors and Affiliations

  • J. D. Verlinde
    • 1
  • A. Kret
    • 1
  1. 1.Department of Bacteriology and Experimental Pathology of the Institute for Preventive MedicineLeiden

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