Diseases of the Colon & Rectum

, Volume 40, Supplement 10, pp S37–S42 | Cite as

Different expressions of sialyl Tn antigen between polypoid and flat-type early colorectal cancers

  • Atsushi Nanashima
  • Tohru Nakagoe
  • Terumitsu Sawai
  • Shiro Nakamura
  • Hiroyuki Yamaguchi
  • Toru Yasutake
  • Hiroyuki Kusano
  • Hiroyoshi Ayabe
Original Contributions


PURPOSE: Sialyl Tn (STn) antigen is a cancer-associated carbohydrate antigen expressed in cancers of the digestive tract. We compared the proportion of specimens of flat-type colorectal cancers expressing STn with that of polypoid cancers, by examining the immunohistochemical reactivity of STn in various morphologic types of early and advanced colorectal cancers. METHODS; A total of 111 biopsies from the colorectal area were examined for STn expression, including 11 adenomas, 58 early cancers, and 42 advanced cancers. Each section was stained immunohistochemically for STn antigen. In each section, we examined STn expression in the cancer area, adjacent mucosa, and normal epithelium. RESULTS: STn expression was detected in 90.9 percent of adenomas, 36.2 percent of early cancers (T1), 64.3 percent of advanced cancers (>T1), and 52 percent of mucosa adjacent to cancer. The morphology of cancer tissue did not influence the number of specimens exhibiting STn antigen expression in mucosa adjacent to cancer cells. STn antigen was rarely expressed in flat or depressed-type early cancers (T1; 7.1 percent), and the expression was higher in moderately than in well-differentiated adenocarcinomas. In advanced cancers (>T1), a similar proportion of protruding and small ulcerative cancers expressed STn. CONCLUSION: Our results suggest that the low expression of STn antigen in flat-type cancers may be the result of different mechanisms of cellular transformation during carcinogenesis from the usual adenoma-carcinoma sequence in colorectal neoplasms.

Key words

Sialyl T-nantigen Flat-type colorectal cancer Mucosa adjacent to cancer 


  1. 1.
    Ajioka Y. Histogenesis and development process of colorectal carcinoma (in Japanese). In: Yoshida Y, ed. Shokakibyo Semina 46 Tokyo: Herusu Syuppan, 1992:49–62.Google Scholar
  2. 2.
    Kasumi A, Kratzer GL. Fast-growing cancer of the colon and rectum. Am Surg 1992;58:383–6.PubMedGoogle Scholar
  3. 3.
    Nagasako K, Sato S, Yashiro K, Suzuki S, Fujimori T. Natural history of superficial colorectal early cancers—from superficial to ulcerated lesions (in Japanese). Shokaki Naishikyo 1993;5:289–96.Google Scholar
  4. 4.
    Crawford BE, Stromeyer FW. Small non polypoid carcinomas of the large intestine. Cancer 1983;9:1760–3.Google Scholar
  5. 5.
    Rubio C, Shetye J. Flat adenoma-adenocarcinoma sequence in the colon of rats. Dis Colon Rectum 1994;37:1300–6.PubMedGoogle Scholar
  6. 6.
    Fujimori T, Satonaka K, Hirayama D,et al. Expression of ras oncogene product (p21) and point mutation of Ki-ras codon 12 in superficial early colorectal cancer (in Japanese). I to Cho 1992;27:111–7.Google Scholar
  7. 7.
    Yamagata S, Muto T, Uchida Y,et al. Lower incidence of K-ras codon 12 mutation in flat colorectal adenomas than in polypoid adenomas. Jpn J Cancer Res 1994;85:147–51.PubMedGoogle Scholar
  8. 8.
    Nanashima A, Tagawa A, Nakagoe T,et al. Relationship between morphological diversity and AgNORs or cathepsin B expression in colorectal cancers. Jpn J Gastroenterol 1996;31:646–53.Google Scholar
  9. 9.
    Nanashima A, Tagawa Y, Morinaga M,et al. Quantitative analysis of numerical chromosome aberrations in various morphological types of colorectal carcinomas. Jpn J Gastroenterol 1996;31:793–800.Google Scholar
  10. 10.
    Sasaki K, Sato T, Kurose A, Uesugi N, Ikeda E. Monosomy of chromosome 18 detected by fluorescencein situ hybridization in colorectal tumors. Cancer 1995;76:1132–8.PubMedGoogle Scholar
  11. 11.
    Filipe MI, Branfoot BM. Abnormal patterns of mucus secretion in apparently normal mucosa of large intestine with carcinoma. Cancer 1974;34:282–90.PubMedGoogle Scholar
  12. 12.
    Dawson PA, Filipe MI. An ultrastructural and histochemical study of the mucus membrane adjacent to and remote from carcinoma of the colon. Cancer 1976;37:2388–98.PubMedGoogle Scholar
  13. 13.
    Matsushita Y, Yamamoto N, Shirahama H,et al. Expression of sulfomucins in normal mucosae, colorectal adenocarcinomas, and metastases. Jpn J Cancer Res 1995;86:1060–7.PubMedGoogle Scholar
  14. 14.
    Yanagida T, Oya M, Ishikawa H. Growth patterns of colorectal carcinoma and mucin property of transitional mucosa (in Japanese). Nippon Shokakibyo Gakkai Zasshi 1996;93:1–9.PubMedGoogle Scholar
  15. 15.
    Itzkowitz SH, Yuan M, Montgomery CK,et al. Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer. Cancer Res 1989;49:197–204.PubMedGoogle Scholar
  16. 16.
    Itzkowitz SH, Bloom EJ, Kokal WA, Modin G, Hakomori S, Kim YS. Sialosyl-Tn: a novel mucin antigen associated with prognosis in colorectal cancer patients. Cancer 1990;66:1960–6.PubMedGoogle Scholar
  17. 17.
    Ørntöft TF, Harving N, Langkilde NC.O-linked mucin-type glycoproteins in normal and malignant colon mucosa: lack of T-antigen expression and accumulation of Tn and sialosyl-Tn antigens in carcinomas. Int J Cancer 1990;45:666–72.PubMedGoogle Scholar
  18. 18.
    Lin TR. Evaluation of transitional mucosa of colon with special reference to sialylated antigen and nuclear DNA content. Acta Med Nagasaki 1992;37:229–33.Google Scholar
  19. 19.
    Itzkowitz SH. Blood group-related carbohydrate antigen expression in malignant and premalignant colonic neoplasms. J Cell Biochem 1992;16:97–101.Google Scholar
  20. 20.
    Jass JR, Filipe MI. Sulphomucins and precancerous lesions of the human stomach. Histopathology 1980;4:271–9.PubMedGoogle Scholar
  21. 21.
    Takahashi I, Maehara Y, Kusumoto T,et al. Predictive value of preoperative serum sialyl Tn antigen levels in prognosis of patients with gastric cancer. Cancer 1993;72:1836–40.PubMedGoogle Scholar
  22. 22.
    Miles DW, Linehan J, Smith P, Filipe I. Expression of sialyl-Tn in gastric cancer: correlation with known prognostic factors. Br J Cancer 1995;71:1074–6.PubMedGoogle Scholar
  23. 23.
    General Rules for Clinical and Pathological Studies on Cancer of the Colon, Rectum and Anus. Japanese Research Society for Cancer of the Colon and Rectum. 5th ed. Tokyo: Kanehara Syuppan, 1994:5–8.Google Scholar
  24. 24.
    Nagasako K, Satonaka K, Hirayama D, Fujimori T, Maeda S. A clinico-pathological study on the mucosal heights of superficial type (Type) early colon cancer (in Japanese). Nippon Shokakibyo Gakkai Zasshi 1991;88:763.PubMedGoogle Scholar
  25. 25.
    Ogata S, Ho I, Chen A,et al. Tumor-associated sialylated antigens are constitutively expressed in normal human colonic mucosa. Cancer Res 1995;55:1869–74.PubMedGoogle Scholar
  26. 26.
    Riddell RH, Levin B. Ultrastructure of the “transitional” mucosa adjacent to large bowel carcinoma. Cancer 1977;40:2509–22.PubMedGoogle Scholar
  27. 27.
    Robey-Cafferty SS, Ro JY, Ordonetz NG, Cleary KR. Transitional mucosa of colon: a morphological, histochemical, and immunohistochemical study. Arch Pathol Lab Med 1990;114:72–5.PubMedGoogle Scholar
  28. 28.
    Jass JR, Sobin LH, eds. Large intestine: the World Health Organization's histological typing of intestinal tumours. 2nd ed. London: Springer, 1989:29–40.Google Scholar
  29. 29.
    Pandey S, Gordon PH, Wang E. Expression of proliferation-specific genes in the mucosa adjacent to colon carcinoma. Dis Colon Rectum 1995;38:462–7.PubMedGoogle Scholar

Copyright information

© American Society of Colon and Rectal Surgeons 1997

Authors and Affiliations

  • Atsushi Nanashima
    • 1
  • Tohru Nakagoe
    • 1
  • Terumitsu Sawai
    • 1
  • Shiro Nakamura
    • 1
  • Hiroyuki Yamaguchi
    • 1
  • Toru Yasutake
    • 1
  • Hiroyuki Kusano
    • 1
  • Hiroyoshi Ayabe
    • 1
  1. 1.The First Department of SurgeryNagasaki University School of MedicineNagasakiJapan

Personalised recommendations