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Preparation of radial hair sections for element profile measurements with a proton microprobe

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Abstract

A new technique has been developed to prepare radial hair sections for element profile analysis using a proton microprobe (PM). The technique makes use of equipment and supplies standard in histological electron microscopy laboratories. In addition to obtaining radial hair slices, adjacent 1 μm thick slices can be acquired for comparative light microscope viewing. With these adjacent optical slices, element profiles of transitional phase and irregularly shaped hair are easily studied. Selected radial sections of C57L/J mouse hair, used to represent this technique, showed element distribution profiles largely similar to their histological counterpart in human hair.

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Author notes

  1. K. A. Stevenson & A. Li-Scholz

    Present address: Empire State College, 845 Central Avenue, 12206, Albany, NY, USA

Authors and Affiliations

  1. Department of Chemistry, Rensselaer Polytechnic Institute, 12181, Troy, New York, (USA)

    K. A. Stevenson & I. L. Preiss

  2. Department of Physics, State University of New York at Albany, 1222, Albany, New York, (USA)

    A. Li-Scholz

Authors
  1. K. A. Stevenson
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  2. I. L. Preiss
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  3. A. Li-Scholz
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Stevenson, K.A., Preiss, I.L. & Li-Scholz, A. Preparation of radial hair sections for element profile measurements with a proton microprobe. Journal of Radioanalytical and Nuclear Chemistry, Articles 148, 63–71 (1991). https://doi.org/10.1007/BF02060547

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  • DOI: https://doi.org/10.1007/BF02060547

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