Abstract
Matrix vesicles are extracellular, membrane-bounded particles which are abundant in the matrix of calcifying cartilage and which are morphologically related to the deposition of mineral in this tissue. The matrix vesicles and cells of the epiphyses of the long bones of fetal calves were liberated by digestion with collagenase and separated by differential centrifugation. Vesicle preparations were found to be lipid-rich and to contain significantly more sphingomyelin and phosphatidyl serine than cellular fractions. The ratio, moles cholesterol to moles total phospholipid, was higher in vesicles than in cells, a finding which is consistent with vesicles having their origins in the plasma membranes of the chondrocytes.
Résumé
Les vésicules matricielles sont des particules extracellulaires, entourées par une membrane, qui sont nombreuses dans la matrice du cartilage en voie de calcification et qui sont en rapport morphologique avec le dépôt minéral. Les vésicules matricielles et les cellules de l'épiphyse d'os longs d'embryons de veaux sont libérées par digestion grâce à la collagénase et séparées par centrifugation différentielle. Les vésicules ainsi obtenues sont riches en lipides et contiennent significativement plus de sphingomyéline et phosphatidyl sérine que les fractions cellulaires. Le rapport moles de cholestérol sur moles de phospholipides totaux est plus élevé dans les vésicules que dans les cellules; ce résultat est en accord avec l'origine des vésicules au niveau des membranes cellulaires des chondrocytes.
Zusammenfassung
Matrixvesikel sind extrazelluläre, membrangebundene Teilchen, welche in der Matrix von verkalkendem Knorpel gehäuft vorkommen und welche in morphologischem Zusammenhang mit der Mineralablagerung in diesem Gewebe stehen. Matrixvesikel und Epiphysenzellen aus Röhrenknochen von Kalbsembryonen wurden mittels Collagenaseverdauung freigesetzt und durch Differential-Zentrifugierung getrennt. Die Vesikelpräparate waren reich an Lipidien und enthielten bedeutend mehr Sphingomyelin und Phosphatidylserin als die Zellfraktionen. Das molare Verhältnis Cholesterol/Gesamtlipide war in den Vesikeln höher als in den Zellen; dieser Befund stimmt mit der Tatsache überein, daß die Vesikel ihren Ursprung in den Plasmamembranen der Chondrozyten haben.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abramson, M. B., Katzman, R., Gregor, H. P.: Aqueous dispersions of phosphatidylserine. Ionic properties. J. biol. Chem.239, 70–76 (1964)
Alcock, N. W.: Calcification of cartilage. Clin. Orthop. and Rel. Res.86, 287–311 (1972)
Ali, S. Y., Sajdera, S. W., Anderson, H. C.: Isolation and characterization of calcifying matrix vesicles from epiphyseal cartilage. Proc. Nat. Acad. Sci. (Wash.)67, 1513–1520 (1970)
Anderson, H. C.: Vesicles associated with calcification in the matrix of epiphyseal cartilage. J. Cell Biol.41, 59–72 (1969)
Anderson, H. C.: Electron microscopic studies of induced cartilage development and calcification. J. Cell Biol.35, 81–101 (1967)
Bernard, G. W.: Ultrastructural observations of initial calcification in dentine and enamel. J. Ultrastruct. Res.41, 1–17 (1972)
Bernard, G. W., Pease, D. C.: An electron microscopic study of initial intramembranous osteogenesis. Amer. J. Anat.125, 271–290 (1969)
Bonucci, E.: Fine structure and histochemistry of calcifying globules in epiphyseal cartilage. Z. Zellforsch.103, 192–217 (1970)
Cartier, P., Picard, J.: La mineralization du cartilage ossifiable. II. Le système ATPasique du cartilage. Bull. Soc. Chim. biol. (Paris)37, 661–675 (1955)
Cotmore, J. M., Nichols, G., Jr., Wuthier, R. E.: Phospholipid—calcium phosphate complex: enhanced calcium migration in the presence of phosphate. Science172, 1339–1341 (1971)
Dawson, R. M. C., Hemington, N., Lindsay, D. B.: The phospholipids of the erythrocyte ‘ghosts’ of various species. Biochem. J.77, 226–230 (1960)
Eisenberg, E., Wuthier, R. E., Frank, R. B., Irving, J. T.: Time study ofin vivo incorporation of32P orthophosphate into phospholipids of chicken epiphyseal tissues. Calcif. Tiss. Res.6, 32–48 (1970)
Eisenmann, D. R., Glick, P. L.: Ultrastructure of initial crystal formation in dentin. J. Ultra struct. Res.41, 18–28 (1972)
Eng, L. F., Noble, E. P.: The maturation of rat brain myelin. Lipids3, 157–162 (1968)
Fleisch, H., Russell, R. G. G., Straumann, F.: Effect of pyrophosphate on hydroxyapatite and its implications in calcium homeostasis. Nature (Lond.)212, 901–903 (1966)
Friede, R. L., Hu, K. H.: Increase in cholesterol along human optic nerve. J. Neurochem.14, 307–315 (1967)
Hendrickson, H. S., Fullington, J. G.: Stabilities of metal complexes of phospholipids. Ca (II), Mg(II), and Ni(II) complexes of phosphotidylserine and triphosphoinositide. Biochemistry4, 1599–1605 (1965)
Irving, J. T.: The sudanophil material at sites of calcification. Arch. Oral Biol.8, 735–745 (1963)
Joos, R. W., Carr, C. W.: The binding of calcium in mixtures of phospholipids. Proc. Soc. Exp. Biol. (N. Y.)124, 1268–1272 (1967)
Klenk, H. D., Choppin, P. W.: Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells. Virology38, 255–268 (1969)
Lowry, O. H., Roseborough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951)
Matsuzawa, I., Anderson, H. C.: Phosphatases of epiphyseal cartilage studied by electron microscopic cytochemical methods. J. Histochem. Cytochem.19, 801–808 (1971)
Robison, R.: The possible significance of hexosephosphoric esters in ossification. Biochem. J.17, 286–293 (1923)
Rouser, G., Kritchevsky, G., Yamamoto, A.: Column chromatographic and associated procedures for separation and determination of phosphatides and glycolipids. In: Lipid chromatographic analysis (G. V. Marinetti, ed.), vol. 1, p. 139–150. New York: Marcel Dekker Inc. 1967
Rouser, G., Siakatos, A. N., Fleischer, S.: Quantitative analysis of phospholipids by thinlayer chromatography and phosphorus analysis of spots. Lipids1, 85–86 (1966)
Sisca, R. F., Provenza, D. V.: Initial dentin formation in human deciduous teeth. An electron microscope study. Calcif. Tiss. Res.9, 1–16 (1972)
Slavkin, C. H., Bringas, P., Jr., Croissant, R., Bavetta, L. A.: Epithelial-mesenchymal interactions during odontogenesis. II. Intercellular matrix vesicles. Mech. Age. Dev.1, 1–23 (1972)
Wattiaux-De Coninck, S., Wattiaux, R.: Nucleosidediphosphatase activity in plasma membrane of rat liver. Biochim. Biophys. Acta (Amst.)183, 118–128 (1969)
Wuthier, R. E.: Phospholipase, an activity in newly mineralizing cartilage and bone. J. Bone Jt. Surg., A54 1117–1118 (1972) (Abstract)
Wuthier, R. E.: Zonal analysis of phospholipids in the epiphyseal cartilage and bone of normal and rachitic chickens and pigs. Calcif. Tiss. Res.8, 36–53 (1971)
Wuthier, R. E.: Lipids of mineralizing epiphyseal tissues in the bovine fetus. J. Lipid Res.9, 68–78 (1968)
Author information
Authors and Affiliations
Additional information
This work was supported by United States Public Health Service grant No. CA-10052 and by a grant from the Arthritis Foundation, Inc., New Yorker Chapter.
Rights and permissions
About this article
Cite this article
Peress, N.S., Anderson, H.C. & Sajdera, S.W. The lipids of matrix vesicles from bovine fetal epiphyseal cartilage. Calc. Tis Res. 14, 275–281 (1974). https://doi.org/10.1007/BF02060301
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02060301